Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract Microbiota
Methanogens, the sole microbes producing methane, are archaea commonly found in human anaerobic microbiota. Methanogens are emerging as opportunistic pathogens associated with dysbiosis and are also detected and cultured in anaerobic abscesses. Their presence in the respiratory tract is yet unknown....
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/1866 |
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| author | Yasmine Hassani Fabienne Brégeon Gérard Aboudharam Michel Drancourt Ghiles Grine |
| author_facet | Yasmine Hassani Fabienne Brégeon Gérard Aboudharam Michel Drancourt Ghiles Grine |
| author_sort | Yasmine Hassani |
| collection | DOAJ |
| description | Methanogens, the sole microbes producing methane, are archaea commonly found in human anaerobic microbiota. Methanogens are emerging as opportunistic pathogens associated with dysbiosis and are also detected and cultured in anaerobic abscesses. Their presence in the respiratory tract is yet unknown. As a preliminary answer, prospective investigation of 908 respiratory tract samples using polyphasic approach combining PCR-sequencing, real-time PCR, fluorescent in situ hybridization (FISH), and methanogens culture was carried out. <i>Methanobrevibacter smithii</i> and <i>Methanobrevibacter oralis</i> DNA sequences, were detected in 21/527 (3.9%) sputum samples, 2/188 (1.06%) bronchoalveolar lavages, and none of 193 tracheo-bronchial aspirations. Further, fluorescence in situ hybridization detected methanogens in three sputum investigated specimens with stick morphology suggesting <i>M. oralis</i> and in another one bronchoalveolar lavage sample investigated, diplococal morphology suggesting <i>M. smithii</i>. These observations extend the known territory of methanogens to the respiratory tract and lay the foundations for further interpretation of their detection as pathogens in any future cases of isolation from bronchoalveolar lavages and the lungs. |
| first_indexed | 2024-03-10T14:32:11Z |
| format | Article |
| id | doaj.art-85f5ab26b1b2446aaae7f52d0bfa37a2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:32:11Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-85f5ab26b1b2446aaae7f52d0bfa37a22023-11-20T22:28:51ZengMDPI AGMicroorganisms2076-26072020-11-01812186610.3390/microorganisms8121866Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract MicrobiotaYasmine Hassani0Fabienne Brégeon1Gérard Aboudharam2Michel Drancourt3Ghiles Grine4IHU Méditerranée Infection, 13005 Marseille, FranceIHU Méditerranée Infection, Aix-Marseille Université, IRD, MEPHI, 13005 Marseille, FranceIHU Méditerranée Infection, Aix-Marseille Université, IRD, MEPHI, 13005 Marseille, FranceIHU Méditerranée Infection, Aix-Marseille Université, IRD, MEPHI, 13005 Marseille, FranceIHU Méditerranée Infection, Aix-Marseille Université, IRD, MEPHI, 13005 Marseille, FranceMethanogens, the sole microbes producing methane, are archaea commonly found in human anaerobic microbiota. Methanogens are emerging as opportunistic pathogens associated with dysbiosis and are also detected and cultured in anaerobic abscesses. Their presence in the respiratory tract is yet unknown. As a preliminary answer, prospective investigation of 908 respiratory tract samples using polyphasic approach combining PCR-sequencing, real-time PCR, fluorescent in situ hybridization (FISH), and methanogens culture was carried out. <i>Methanobrevibacter smithii</i> and <i>Methanobrevibacter oralis</i> DNA sequences, were detected in 21/527 (3.9%) sputum samples, 2/188 (1.06%) bronchoalveolar lavages, and none of 193 tracheo-bronchial aspirations. Further, fluorescence in situ hybridization detected methanogens in three sputum investigated specimens with stick morphology suggesting <i>M. oralis</i> and in another one bronchoalveolar lavage sample investigated, diplococal morphology suggesting <i>M. smithii</i>. These observations extend the known territory of methanogens to the respiratory tract and lay the foundations for further interpretation of their detection as pathogens in any future cases of isolation from bronchoalveolar lavages and the lungs.https://www.mdpi.com/2076-2607/8/12/1866methanogens<i>Methanobrevibacter smithii</i><i>Methanobrevibacter oralis</i>respiratory tractmicrobiota |
| spellingShingle | Yasmine Hassani Fabienne Brégeon Gérard Aboudharam Michel Drancourt Ghiles Grine Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract Microbiota Microorganisms methanogens <i>Methanobrevibacter smithii</i> <i>Methanobrevibacter oralis</i> respiratory tract microbiota |
| title | Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract Microbiota |
| title_full | Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract Microbiota |
| title_fullStr | Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract Microbiota |
| title_full_unstemmed | Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract Microbiota |
| title_short | Detection of <i>Methanobrevobacter smithii</i> and <i>Methanobrevibacter oralis</i> in Lower Respiratory Tract Microbiota |
| title_sort | detection of i methanobrevobacter smithii i and i methanobrevibacter oralis i in lower respiratory tract microbiota |
| topic | methanogens <i>Methanobrevibacter smithii</i> <i>Methanobrevibacter oralis</i> respiratory tract microbiota |
| url | https://www.mdpi.com/2076-2607/8/12/1866 |
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