Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7
Abstract Background Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industrial application depends on the energetics and biomass, especially ATP ava...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-021-02051-4 |
| _version_ | 1828339141793808384 |
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| author | Xiangfei Li Rumeng Han Teng Bao Tolbert Osire Xian Zhang Meijuan Xu Taowei Yang Zhiming Rao |
| author_facet | Xiangfei Li Rumeng Han Teng Bao Tolbert Osire Xian Zhang Meijuan Xu Taowei Yang Zhiming Rao |
| author_sort | Xiangfei Li |
| collection | DOAJ |
| description | Abstract Background Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industrial application depends on the energetics and biomass, especially ATP availability. Results This study found that exogenous addition of citrulline promoted accumulation of ATP, increased specific growth rate, and reduced the doubling time of C. carboxidivorans P7. In heterotrophic fermentation experiments, the addition of citrulline increased intracellular ATP by 3.39-fold, significantly enhancing the production of total alcohol (ethanol + butanol) by 20%. Moreover, in the syngas fermentation experiments, the addition of citrulline improved the level of intracellular ATP and the biomass by 80.5% and 31.6%, respectively, resulting in an 18.6% and 60.3% increase in ethanol and the alcohol/acid production ratio, respectively. Conclusions This is the first report that citrulline could promote the growth of C. carboxidivorans P7 and increase the level of intracellular ATP, which is of great significance for the use of C. carboxidivorans P7 to synthesize biofuels. |
| first_indexed | 2024-04-13T22:39:00Z |
| format | Article |
| id | doaj.art-85f84381cf79489d8e8dbad618e85f9a |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:39:00Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-85f84381cf79489d8e8dbad618e85f9a2022-12-22T02:26:41ZengBMCBiotechnology for Biofuels1754-68342021-10-0114111010.1186/s13068-021-02051-4Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7Xiangfei Li0Rumeng Han1Teng Bao2Tolbert Osire3Xian Zhang4Meijuan Xu5Taowei Yang6Zhiming Rao7The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan UniversityDepartment of Chemical and Biomolecular Engineering, The Ohio State UniversityThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan UniversityAbstract Background Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industrial application depends on the energetics and biomass, especially ATP availability. Results This study found that exogenous addition of citrulline promoted accumulation of ATP, increased specific growth rate, and reduced the doubling time of C. carboxidivorans P7. In heterotrophic fermentation experiments, the addition of citrulline increased intracellular ATP by 3.39-fold, significantly enhancing the production of total alcohol (ethanol + butanol) by 20%. Moreover, in the syngas fermentation experiments, the addition of citrulline improved the level of intracellular ATP and the biomass by 80.5% and 31.6%, respectively, resulting in an 18.6% and 60.3% increase in ethanol and the alcohol/acid production ratio, respectively. Conclusions This is the first report that citrulline could promote the growth of C. carboxidivorans P7 and increase the level of intracellular ATP, which is of great significance for the use of C. carboxidivorans P7 to synthesize biofuels.https://doi.org/10.1186/s13068-021-02051-4Clostridium carboxidivoransCitrullineSyngas fermentationAlcohol/acid ratio |
| spellingShingle | Xiangfei Li Rumeng Han Teng Bao Tolbert Osire Xian Zhang Meijuan Xu Taowei Yang Zhiming Rao Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7 Biotechnology for Biofuels Clostridium carboxidivorans Citrulline Syngas fermentation Alcohol/acid ratio |
| title | Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7 |
| title_full | Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7 |
| title_fullStr | Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7 |
| title_full_unstemmed | Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7 |
| title_short | Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7 |
| title_sort | citrulline deiminase pathway provides atp and boosts growth of clostridium carboxidivorans p7 |
| topic | Clostridium carboxidivorans Citrulline Syngas fermentation Alcohol/acid ratio |
| url | https://doi.org/10.1186/s13068-021-02051-4 |
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