Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth
Beneficial soil microorganisms influence nutrient recycling, soil fertility, plant growth, and productivity and reduce chemical fertilizer application. This study aimed to isolate bacteria from non-agricultural soils in the Al-Ahsa region and characterize the bacteria with the best biostimulating ch...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/6/873 |
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| author | Heba Adel AlAli Ashraf Khalifa Mohammed Almalki |
| author_facet | Heba Adel AlAli Ashraf Khalifa Mohammed Almalki |
| author_sort | Heba Adel AlAli |
| collection | DOAJ |
| description | Beneficial soil microorganisms influence nutrient recycling, soil fertility, plant growth, and productivity and reduce chemical fertilizer application. This study aimed to isolate bacteria from non-agricultural soils in the Al-Ahsa region and characterize the bacteria with the best biostimulating characteristics at the physiological, biochemical, and molecular level. DPM17, a bacterial isolate, promotes plant growth through phosphate solubilization, nitrogen fixation, and ammonia production. DPM17 also produces the phytohormones, indole acetic acid (IAA; 4.516 μg mL<sup>−1</sup>) and gibberellin (1.33 µg mL<sup>−1</sup>), and ammonia (0.06 µg mL<sup>−1</sup>). Additionally, DPM17 grows in the presence of up to 10% NaCl, indicating its halophilic nature. DPM17 was identified as <i>Bacillus</i> <i>baekryungensis</i> based on comparative sequence analysis of the 16S rRNA gene, and neighbor-joining phylogenetic analyses indicated that DPM17 was 96.51% identified to <i>Bacillus</i> sp. DPM17 inoculation substantially improved <i>Abelmoschus esculentus</i> (okra) root length, lateral root count, and dry weight from 7.03 to 9.41 (<i>p</i> = 0.03), 3.2 to 7.2, and 6 to 13 mg (<i>p</i> = 0.032), respectively. The results suggest that DPM17 enhances plant growth and can be exploited to develop efficient formulations for sustainable agriculture and food security in Saudi Arabia. |
| first_indexed | 2024-03-10T00:40:25Z |
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| id | doaj.art-85fa7ea215e4429396230e8b789ca333 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T00:40:25Z |
| publishDate | 2022-06-01 |
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| series | Agriculture |
| spelling | doaj.art-85fa7ea215e4429396230e8b789ca3332023-11-23T15:08:04ZengMDPI AGAgriculture2077-04722022-06-0112687310.3390/agriculture12060873Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant GrowthHeba Adel AlAli0Ashraf Khalifa1Mohammed Almalki2Al Bilad Bank Scholarly Chair for Food Security in Saudi Arabia, the Deanship of Scientific Research, the Vice Presidency for Graduate Studies and Scientific Research, Al-Ahsa 31982, Saudi ArabiaAl Bilad Bank Scholarly Chair for Food Security in Saudi Arabia, the Deanship of Scientific Research, the Vice Presidency for Graduate Studies and Scientific Research, Al-Ahsa 31982, Saudi ArabiaAl Bilad Bank Scholarly Chair for Food Security in Saudi Arabia, the Deanship of Scientific Research, the Vice Presidency for Graduate Studies and Scientific Research, Al-Ahsa 31982, Saudi ArabiaBeneficial soil microorganisms influence nutrient recycling, soil fertility, plant growth, and productivity and reduce chemical fertilizer application. This study aimed to isolate bacteria from non-agricultural soils in the Al-Ahsa region and characterize the bacteria with the best biostimulating characteristics at the physiological, biochemical, and molecular level. DPM17, a bacterial isolate, promotes plant growth through phosphate solubilization, nitrogen fixation, and ammonia production. DPM17 also produces the phytohormones, indole acetic acid (IAA; 4.516 μg mL<sup>−1</sup>) and gibberellin (1.33 µg mL<sup>−1</sup>), and ammonia (0.06 µg mL<sup>−1</sup>). Additionally, DPM17 grows in the presence of up to 10% NaCl, indicating its halophilic nature. DPM17 was identified as <i>Bacillus</i> <i>baekryungensis</i> based on comparative sequence analysis of the 16S rRNA gene, and neighbor-joining phylogenetic analyses indicated that DPM17 was 96.51% identified to <i>Bacillus</i> sp. DPM17 inoculation substantially improved <i>Abelmoschus esculentus</i> (okra) root length, lateral root count, and dry weight from 7.03 to 9.41 (<i>p</i> = 0.03), 3.2 to 7.2, and 6 to 13 mg (<i>p</i> = 0.032), respectively. The results suggest that DPM17 enhances plant growth and can be exploited to develop efficient formulations for sustainable agriculture and food security in Saudi Arabia.https://www.mdpi.com/2077-0472/12/6/873<i>Bacillus</i><i>Abelmoschus esculentus</i>biostimulationnon-agricultural soils |
| spellingShingle | Heba Adel AlAli Ashraf Khalifa Mohammed Almalki Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth Agriculture <i>Bacillus</i> <i>Abelmoschus esculentus</i> biostimulation non-agricultural soils |
| title | Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth |
| title_full | Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth |
| title_fullStr | Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth |
| title_full_unstemmed | Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth |
| title_short | Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth |
| title_sort | plant growth promoting bacterium from non agricultural soil improves okra plant growth |
| topic | <i>Bacillus</i> <i>Abelmoschus esculentus</i> biostimulation non-agricultural soils |
| url | https://www.mdpi.com/2077-0472/12/6/873 |
| work_keys_str_mv | AT hebaadelalali plantgrowthpromotingbacteriumfromnonagriculturalsoilimprovesokraplantgrowth AT ashrafkhalifa plantgrowthpromotingbacteriumfromnonagriculturalsoilimprovesokraplantgrowth AT mohammedalmalki plantgrowthpromotingbacteriumfromnonagriculturalsoilimprovesokraplantgrowth |