Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i>
Pneumonia is a cause of high morbidity and mortality in humans. Animal models are indispensable to investigate the complex cellular interactions during lung injury and repair in vivo. The time sequence of lesion development and regeneration is described after endobronchial inoculation of calves with...
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/8/2817 |
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| author | Elisabeth M. Liebler-Tenorio Jacqueline Lambertz Carola Ostermann Konrad Sachse Petra Reinhold |
| author_facet | Elisabeth M. Liebler-Tenorio Jacqueline Lambertz Carola Ostermann Konrad Sachse Petra Reinhold |
| author_sort | Elisabeth M. Liebler-Tenorio |
| collection | DOAJ |
| description | Pneumonia is a cause of high morbidity and mortality in humans. Animal models are indispensable to investigate the complex cellular interactions during lung injury and repair in vivo. The time sequence of lesion development and regeneration is described after endobronchial inoculation of calves with <i>Chlamydia psittaci.</i> Calves were necropsied 2–37 days after inoculation (dpi). Lesions and presence of <i>Chlamydia psittaci</i> were investigated using histology and immunohistochemistry. Calves developed bronchopneumonia at the sites of inoculation. Initially, <i>Chlamydia psittaci</i> replicated in type 1 alveolar epithelial cells followed by an influx of neutrophils, vascular leakage, fibrinous exudation, thrombosis and lobular pulmonary necrosis. Lesions were most extensive at 4 dpi. Beginning at 7 dpi, the number of chlamydial inclusions declined and proliferation of cuboidal alveolar epithelial cells and sprouting of capillaries were seen at the periphery of necrotic tissue. At 14 dpi, most of the necrosis had been replaced with alveoli lined with cuboidal epithelial cells resembling type 2 alveolar epithelial cells and mild fibrosis, and hyperplasia of organized lymphoid tissue were observed. At 37 dpi, regeneration of pulmonary tissue was nearly complete and only small foci of remodeling remained. The well-defined time course of development and regeneration of necrotizing pneumonia allows correlation of morphological findings with clinical data or treatment regimen. |
| first_indexed | 2024-03-10T20:23:18Z |
| format | Article |
| id | doaj.art-85fa962b25e64374b46d833fe9165d9c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:23:18Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-85fa962b25e64374b46d833fe9165d9c2023-11-19T21:58:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218281710.3390/ijms21082817Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i>Elisabeth M. Liebler-Tenorio0Jacqueline Lambertz1Carola Ostermann2Konrad Sachse3Petra Reinhold4Institute for Molecular Pathogenesis, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburgerstr. 96a, 07743 Jena, GermanyInstitute for Molecular Pathogenesis, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburgerstr. 96a, 07743 Jena, GermanyInstitute for Molecular Pathogenesis, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburgerstr. 96a, 07743 Jena, GermanyInstitute for Molecular Pathogenesis, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburgerstr. 96a, 07743 Jena, GermanyInstitute for Molecular Pathogenesis, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburgerstr. 96a, 07743 Jena, GermanyPneumonia is a cause of high morbidity and mortality in humans. Animal models are indispensable to investigate the complex cellular interactions during lung injury and repair in vivo. The time sequence of lesion development and regeneration is described after endobronchial inoculation of calves with <i>Chlamydia psittaci.</i> Calves were necropsied 2–37 days after inoculation (dpi). Lesions and presence of <i>Chlamydia psittaci</i> were investigated using histology and immunohistochemistry. Calves developed bronchopneumonia at the sites of inoculation. Initially, <i>Chlamydia psittaci</i> replicated in type 1 alveolar epithelial cells followed by an influx of neutrophils, vascular leakage, fibrinous exudation, thrombosis and lobular pulmonary necrosis. Lesions were most extensive at 4 dpi. Beginning at 7 dpi, the number of chlamydial inclusions declined and proliferation of cuboidal alveolar epithelial cells and sprouting of capillaries were seen at the periphery of necrotic tissue. At 14 dpi, most of the necrosis had been replaced with alveoli lined with cuboidal epithelial cells resembling type 2 alveolar epithelial cells and mild fibrosis, and hyperplasia of organized lymphoid tissue were observed. At 37 dpi, regeneration of pulmonary tissue was nearly complete and only small foci of remodeling remained. The well-defined time course of development and regeneration of necrotizing pneumonia allows correlation of morphological findings with clinical data or treatment regimen.https://www.mdpi.com/1422-0067/21/8/2817lungpneumonianecrotizingregenerationmodelbovine |
| spellingShingle | Elisabeth M. Liebler-Tenorio Jacqueline Lambertz Carola Ostermann Konrad Sachse Petra Reinhold Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i> International Journal of Molecular Sciences lung pneumonia necrotizing regeneration model bovine |
| title | Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i> |
| title_full | Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i> |
| title_fullStr | Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i> |
| title_full_unstemmed | Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i> |
| title_short | Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with <i>Chlamydia psittaci</i> |
| title_sort | regeneration of pulmonary tissue in a calf model of fibrinonecrotic bronchopneumonia induced by experimental infection with i chlamydia psittaci i |
| topic | lung pneumonia necrotizing regeneration model bovine |
| url | https://www.mdpi.com/1422-0067/21/8/2817 |
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