Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device
Here we report the improved Cyclo olefin polymer (COP) microfluidic chip and polymerase chain reaction (PCR) amplification system for point-of-care testing (POCT) in rapid detection of Carbapenem-resistant Enterobacteriaceae (CRE). The PCR solution and thermal cycling is controlled by the relative g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/4/1225 |
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| author | Wilfred Villariza Espulgar Masato Saito Kazuya Takahashi Sakiko Ushiro Norihisa Yamamoto Yukihiro Akeda Shigeto Hamaguchi Kazunori Tomono Eiichi Tamiya |
| author_facet | Wilfred Villariza Espulgar Masato Saito Kazuya Takahashi Sakiko Ushiro Norihisa Yamamoto Yukihiro Akeda Shigeto Hamaguchi Kazunori Tomono Eiichi Tamiya |
| author_sort | Wilfred Villariza Espulgar |
| collection | DOAJ |
| description | Here we report the improved Cyclo olefin polymer (COP) microfluidic chip and polymerase chain reaction (PCR) amplification system for point-of-care testing (POCT) in rapid detection of Carbapenem-resistant Enterobacteriaceae (CRE). The PCR solution and thermal cycling is controlled by the relative gravitational acceleration (7G) only and is expected to pose minimal problem in operation by non-expert users. Detection is based on identifying the presence of carbapenemase encoding gene through the corresponding fluorescence signal after amplification. For preliminary tests, the device has been demonstrated to detect <i>bla</i><sub>IMP-6</sub> from patients stool samples. From the prepared samples, 96.4 fg/µL was detected with good certainty within 15 min (~106 thermocycles,) which is significantly faster than the conventional culture plate method. Moreover, the device is expected to detect other target genes in parallel as determination of the presence of <i>bla</i><sub>NDM-1</sub> and <i>bla</i><sub>OXA-23</sub> from control samples has also been demonstrated. With the rising threat of drug-resistant bacteria in global healthcare, this technology can greatly aid the health sector by enabling the appropriate use of antibiotics, accelerating the treatment of carriers, and suppressing the spread. |
| first_indexed | 2024-03-09T04:58:15Z |
| format | Article |
| id | doaj.art-85fae5b8eadf4b3899e5b694de83332a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T04:58:15Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-85fae5b8eadf4b3899e5b694de83332a2023-12-03T13:03:37ZengMDPI AGSensors1424-82202021-02-01214122510.3390/s21041225Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR DeviceWilfred Villariza Espulgar0Masato Saito1Kazuya Takahashi2Sakiko Ushiro3Norihisa Yamamoto4Yukihiro Akeda5Shigeto Hamaguchi6Kazunori Tomono7Eiichi Tamiya8Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Infection Control and Prevention, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Infection Control and Prevention, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Infection Control and Prevention, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Infection Control and Prevention, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanHere we report the improved Cyclo olefin polymer (COP) microfluidic chip and polymerase chain reaction (PCR) amplification system for point-of-care testing (POCT) in rapid detection of Carbapenem-resistant Enterobacteriaceae (CRE). The PCR solution and thermal cycling is controlled by the relative gravitational acceleration (7G) only and is expected to pose minimal problem in operation by non-expert users. Detection is based on identifying the presence of carbapenemase encoding gene through the corresponding fluorescence signal after amplification. For preliminary tests, the device has been demonstrated to detect <i>bla</i><sub>IMP-6</sub> from patients stool samples. From the prepared samples, 96.4 fg/µL was detected with good certainty within 15 min (~106 thermocycles,) which is significantly faster than the conventional culture plate method. Moreover, the device is expected to detect other target genes in parallel as determination of the presence of <i>bla</i><sub>NDM-1</sub> and <i>bla</i><sub>OXA-23</sub> from control samples has also been demonstrated. With the rising threat of drug-resistant bacteria in global healthcare, this technology can greatly aid the health sector by enabling the appropriate use of antibiotics, accelerating the treatment of carriers, and suppressing the spread.https://www.mdpi.com/1424-8220/21/4/1225drug-resistant geneIMP-type CPEPOCTPCRmicrofluidics |
| spellingShingle | Wilfred Villariza Espulgar Masato Saito Kazuya Takahashi Sakiko Ushiro Norihisa Yamamoto Yukihiro Akeda Shigeto Hamaguchi Kazunori Tomono Eiichi Tamiya Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device Sensors drug-resistant gene IMP-type CPE POCT PCR microfluidics |
| title | Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device |
| title_full | Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device |
| title_fullStr | Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device |
| title_full_unstemmed | Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device |
| title_short | Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device |
| title_sort | deskilled and rapid drug resistant gene detection by centrifugal force assisted thermal convection pcr device |
| topic | drug-resistant gene IMP-type CPE POCT PCR microfluidics |
| url | https://www.mdpi.com/1424-8220/21/4/1225 |
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