Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats
Objective: Diabetes in pregnancy is a prevalent disease that can affect the central nervous system of the fetus byhyperglycemia. This study aimed to investigate the impact of maternal diabetes on neuronal apoptosis in the superiorcolliculus (SC) and the lateral geniculate nucleus (LGN) in male neona...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2023-08-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_705397_816b6c42b833c219246aeb1ea8563e92.pdf |
| _version_ | 1827856922132348928 |
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| author | Nasim Alipour Somaye Fallahnezhad Javad Bagheri Hamideh Babaloo Fatemeh Tahmasebi Ghasem Sazegar Hossein Haghir |
| author_facet | Nasim Alipour Somaye Fallahnezhad Javad Bagheri Hamideh Babaloo Fatemeh Tahmasebi Ghasem Sazegar Hossein Haghir |
| author_sort | Nasim Alipour |
| collection | DOAJ |
| description | Objective: Diabetes in pregnancy is a prevalent disease that can affect the central nervous system of the fetus byhyperglycemia. This study aimed to investigate the impact of maternal diabetes on neuronal apoptosis in the superiorcolliculus (SC) and the lateral geniculate nucleus (LGN) in male neonates born to diabetic mothers.Materials and Methods: In this experimental study, female adult rats were separated into three groups: control,diabetic (induced using an intraperitoneal injection of streptozotocin), and insulin-treated diabetic [diabetes controlledby subcutaneous neutral protamine hagedorn (NPH)-insulin injection]. Male neonates from each group were euthanizedon 0, 7, and 14 postnatal days (P0, P7, and P14, respectively), and apoptotic cells were identified using TUNEL staining.Results: The numerical density per unit area (NA) of apoptotic cells was significantly higher in SC and the dorsalLGN (dLGN) in neonates born to the diabetic rats compared to the control group at P0, P7, and P14. However, insulintreatment normalized the number of apoptotic cells.Conclusion: This study demonstrated that maternal diabetes increased apoptosis in dLGN and SC of male neonatesat P0, P7, and P14. |
| first_indexed | 2024-03-12T12:34:10Z |
| format | Article |
| id | doaj.art-85fc76b62eb94826bbde19927e3b433c |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-03-12T12:34:10Z |
| publishDate | 2023-08-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-85fc76b62eb94826bbde19927e3b433c2023-08-29T06:08:18ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142023-08-0125856456910.22074/cellj.2023.1989649.1232705397Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic RatsNasim Alipour0Somaye Fallahnezhad1Javad Bagheri2Hamideh Babaloo3Fatemeh Tahmasebi4Ghasem Sazegar5Hossein Haghir6Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranNervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective: Diabetes in pregnancy is a prevalent disease that can affect the central nervous system of the fetus byhyperglycemia. This study aimed to investigate the impact of maternal diabetes on neuronal apoptosis in the superiorcolliculus (SC) and the lateral geniculate nucleus (LGN) in male neonates born to diabetic mothers.Materials and Methods: In this experimental study, female adult rats were separated into three groups: control,diabetic (induced using an intraperitoneal injection of streptozotocin), and insulin-treated diabetic [diabetes controlledby subcutaneous neutral protamine hagedorn (NPH)-insulin injection]. Male neonates from each group were euthanizedon 0, 7, and 14 postnatal days (P0, P7, and P14, respectively), and apoptotic cells were identified using TUNEL staining.Results: The numerical density per unit area (NA) of apoptotic cells was significantly higher in SC and the dorsalLGN (dLGN) in neonates born to the diabetic rats compared to the control group at P0, P7, and P14. However, insulintreatment normalized the number of apoptotic cells.Conclusion: This study demonstrated that maternal diabetes increased apoptosis in dLGN and SC of male neonatesat P0, P7, and P14.https://www.celljournal.org/article_705397_816b6c42b833c219246aeb1ea8563e92.pdfapoptosislateral geniculate nucleusmaternal diabetesrat brainsuperior colliculus |
| spellingShingle | Nasim Alipour Somaye Fallahnezhad Javad Bagheri Hamideh Babaloo Fatemeh Tahmasebi Ghasem Sazegar Hossein Haghir Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats Cell Journal apoptosis lateral geniculate nucleus maternal diabetes rat brain superior colliculus |
| title | Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats |
| title_full | Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats |
| title_fullStr | Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats |
| title_full_unstemmed | Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats |
| title_short | Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats |
| title_sort | increased apoptosis in subcortical regions of the visual pathway in offspring born to diabetic rats |
| topic | apoptosis lateral geniculate nucleus maternal diabetes rat brain superior colliculus |
| url | https://www.celljournal.org/article_705397_816b6c42b833c219246aeb1ea8563e92.pdf |
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