<i>Candida</i> spp. DNA Extraction in the Age of Molecular Diagnosis
The standard procedure for the detection of candidemia is blood culture, a method that might require 3–5 days for a positive result. Compared with culturing, molecular diagnosis techniques can provide faster diagnosis. The current paper aimed to present the main strengths and constraints of current...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/4/818 |
| _version_ | 1797604249911164928 |
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| author | Smaranda Ioana Codreanu Cristina Nicoleta Ciurea |
| author_facet | Smaranda Ioana Codreanu Cristina Nicoleta Ciurea |
| author_sort | Smaranda Ioana Codreanu |
| collection | DOAJ |
| description | The standard procedure for the detection of candidemia is blood culture, a method that might require 3–5 days for a positive result. Compared with culturing, molecular diagnosis techniques can provide faster diagnosis. The current paper aimed to present the main strengths and constraints of current molecular techniques for <i>Candida</i> spp. DNA extraction, analyzing their efficiency from a time, price, and ease of usage point of view. A comprehensive search was conducted using the PubMed NIH database for peer-reviewed full-text articles published before October 2022. The studies provided adequate data on the diagnosis of the infection with the <i>Candida</i> spp. DNA extraction is a relevant step in yielding pure qualitative DNA to be amplified in molecular diagnostic techniques. The most used fungal DNA extraction strategies are: mechanical (bead beating, ultrasonication, steel-bullet beating), enzymatic (proteinase K, lysozyme, lyticase), and chemical extraction (formic acid, liquid nitrogen, ammonium chloride). More clinical studies are needed to formulate adequate guidelines for fungal DNA extraction as the current paper highlighted discrepancies in the reported outcome. |
| first_indexed | 2024-03-11T04:43:39Z |
| format | Article |
| id | doaj.art-85ff1946fcb548a1980948600c438cdd |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:39Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-85ff1946fcb548a1980948600c438cdd2023-11-17T20:30:59ZengMDPI AGMicroorganisms2076-26072023-03-0111481810.3390/microorganisms11040818<i>Candida</i> spp. DNA Extraction in the Age of Molecular DiagnosisSmaranda Ioana Codreanu0Cristina Nicoleta Ciurea1Faculty of Medicine, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mures, 38 Gheorghe Marinescu Street, 540139 Târgu Mures, RomaniaDepartment of Microbiology, Faculty of Medicine, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mures, 38 Gheorghe Marinescu Street, 540139 Târgu Mures, RomaniaThe standard procedure for the detection of candidemia is blood culture, a method that might require 3–5 days for a positive result. Compared with culturing, molecular diagnosis techniques can provide faster diagnosis. The current paper aimed to present the main strengths and constraints of current molecular techniques for <i>Candida</i> spp. DNA extraction, analyzing their efficiency from a time, price, and ease of usage point of view. A comprehensive search was conducted using the PubMed NIH database for peer-reviewed full-text articles published before October 2022. The studies provided adequate data on the diagnosis of the infection with the <i>Candida</i> spp. DNA extraction is a relevant step in yielding pure qualitative DNA to be amplified in molecular diagnostic techniques. The most used fungal DNA extraction strategies are: mechanical (bead beating, ultrasonication, steel-bullet beating), enzymatic (proteinase K, lysozyme, lyticase), and chemical extraction (formic acid, liquid nitrogen, ammonium chloride). More clinical studies are needed to formulate adequate guidelines for fungal DNA extraction as the current paper highlighted discrepancies in the reported outcome.https://www.mdpi.com/2076-2607/11/4/818DNA extractioncandidemiamolecular diagnosticsPCRCandida |
| spellingShingle | Smaranda Ioana Codreanu Cristina Nicoleta Ciurea <i>Candida</i> spp. DNA Extraction in the Age of Molecular Diagnosis Microorganisms DNA extraction candidemia molecular diagnostics PCR Candida |
| title | <i>Candida</i> spp. DNA Extraction in the Age of Molecular Diagnosis |
| title_full | <i>Candida</i> spp. DNA Extraction in the Age of Molecular Diagnosis |
| title_fullStr | <i>Candida</i> spp. DNA Extraction in the Age of Molecular Diagnosis |
| title_full_unstemmed | <i>Candida</i> spp. DNA Extraction in the Age of Molecular Diagnosis |
| title_short | <i>Candida</i> spp. DNA Extraction in the Age of Molecular Diagnosis |
| title_sort | i candida i spp dna extraction in the age of molecular diagnosis |
| topic | DNA extraction candidemia molecular diagnostics PCR Candida |
| url | https://www.mdpi.com/2076-2607/11/4/818 |
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