Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis
In response to pathological stimulation, methylation status conversion of the genome drives changes of cell feature and is able to promote disease development. Yet the role of methylation in the development of thyroid-associated ophthalmopathy (TAO) remains to be evaluated. Overexpansion of orbital...
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Frontiers Media S.A.
2021-09-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.716871/full |
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| author | Yu Liang Yu Liang Yu Liang Yu Liang Sijia Ding Xiying Wang Xiying Wang Xiying Wang Xiying Wang Chunchun Hu Chunchun Hu Chunchun Hu Chunchun Hu Yihan Zhang Yihan Zhang Yihan Zhang Yihan Zhang Yan Hu Yan Hu Yan Hu Yan Hu Yuye Zhang Yuye Zhang Yuye Zhang Yuye Zhang Hongyu Kong Hongyu Kong Hongyu Kong Hongyu Kong Weiyi Xia Weiyi Xia Weiyi Xia Weiyi Xia Qinghe Jing Qinghe Jing Qinghe Jing Qinghe Jing Yuxiang Hu Yuxiang Hu Yuxiang Hu Yuxiang Hu Chen Zhao Chen Zhao Chen Zhao Chen Zhao Lianqun Wu Lianqun Wu Lianqun Wu Lianqun Wu |
| author_facet | Yu Liang Yu Liang Yu Liang Yu Liang Sijia Ding Xiying Wang Xiying Wang Xiying Wang Xiying Wang Chunchun Hu Chunchun Hu Chunchun Hu Chunchun Hu Yihan Zhang Yihan Zhang Yihan Zhang Yihan Zhang Yan Hu Yan Hu Yan Hu Yan Hu Yuye Zhang Yuye Zhang Yuye Zhang Yuye Zhang Hongyu Kong Hongyu Kong Hongyu Kong Hongyu Kong Weiyi Xia Weiyi Xia Weiyi Xia Weiyi Xia Qinghe Jing Qinghe Jing Qinghe Jing Qinghe Jing Yuxiang Hu Yuxiang Hu Yuxiang Hu Yuxiang Hu Chen Zhao Chen Zhao Chen Zhao Chen Zhao Lianqun Wu Lianqun Wu Lianqun Wu Lianqun Wu |
| author_sort | Yu Liang |
| collection | DOAJ |
| description | In response to pathological stimulation, methylation status conversion of the genome drives changes of cell feature and is able to promote disease development. Yet the role of methylation in the development of thyroid-associated ophthalmopathy (TAO) remains to be evaluated. Overexpansion of orbital tissue is the key feature of TAO. In this study, the methylation profile of orbital adipose/connective tissue from TAO patients and normal individuals were compared. After screening 3,739 differentially methylated probes, the distribution and properties of these probes were analyzed. Furthermore, enriched biological functions of these genes associated with differential methylation and the relationship between their methylation status and expression profile were also identified, including PTPRU and VCAM-1. According to our results, methylation was involved in disregulated immune response and inflammation in TAO and might contribute to activation of fibroblast and adipogenesis, leading to the expansion of orbital tissue. Neuropathy and neurobehavioral symptoms were also potentially associated with methylation. These results may help to extend the understanding of methylation in TAO and provide more insights into diagnosis and treatment of patients. |
| first_indexed | 2024-12-22T05:05:42Z |
| format | Article |
| id | doaj.art-86007a945e7648989a466985339acbb4 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
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| spelling | doaj.art-86007a945e7648989a466985339acbb42022-12-21T18:38:07ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-09-01910.3389/fcell.2021.716871716871Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation AnalysisYu Liang0Yu Liang1Yu Liang2Yu Liang3Sijia Ding4Xiying Wang5Xiying Wang6Xiying Wang7Xiying Wang8Chunchun Hu9Chunchun Hu10Chunchun Hu11Chunchun Hu12Yihan Zhang13Yihan Zhang14Yihan Zhang15Yihan Zhang16Yan Hu17Yan Hu18Yan Hu19Yan Hu20Yuye Zhang21Yuye Zhang22Yuye Zhang23Yuye Zhang24Hongyu Kong25Hongyu Kong26Hongyu Kong27Hongyu Kong28Weiyi Xia29Weiyi Xia30Weiyi Xia31Weiyi Xia32Qinghe Jing33Qinghe Jing34Qinghe Jing35Qinghe Jing36Yuxiang Hu37Yuxiang Hu38Yuxiang Hu39Yuxiang Hu40Chen Zhao41Chen Zhao42Chen Zhao43Chen Zhao44Lianqun Wu45Lianqun Wu46Lianqun Wu47Lianqun Wu48Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaDepartment of Phase 1 Clinical Trial Unit, The First Affiliated Hospital with Nanjing Medical University, Nanjing, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaEye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Myopia (Fudan University), Shanghai, ChinaKey Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, ChinaIn response to pathological stimulation, methylation status conversion of the genome drives changes of cell feature and is able to promote disease development. Yet the role of methylation in the development of thyroid-associated ophthalmopathy (TAO) remains to be evaluated. Overexpansion of orbital tissue is the key feature of TAO. In this study, the methylation profile of orbital adipose/connective tissue from TAO patients and normal individuals were compared. After screening 3,739 differentially methylated probes, the distribution and properties of these probes were analyzed. Furthermore, enriched biological functions of these genes associated with differential methylation and the relationship between their methylation status and expression profile were also identified, including PTPRU and VCAM-1. According to our results, methylation was involved in disregulated immune response and inflammation in TAO and might contribute to activation of fibroblast and adipogenesis, leading to the expansion of orbital tissue. Neuropathy and neurobehavioral symptoms were also potentially associated with methylation. These results may help to extend the understanding of methylation in TAO and provide more insights into diagnosis and treatment of patients.https://www.frontiersin.org/articles/10.3389/fcell.2021.716871/fullthyroid-associated ophthalmopathymethylationorbital adipose/connective tissuemethylation arraypathology |
| spellingShingle | Yu Liang Yu Liang Yu Liang Yu Liang Sijia Ding Xiying Wang Xiying Wang Xiying Wang Xiying Wang Chunchun Hu Chunchun Hu Chunchun Hu Chunchun Hu Yihan Zhang Yihan Zhang Yihan Zhang Yihan Zhang Yan Hu Yan Hu Yan Hu Yan Hu Yuye Zhang Yuye Zhang Yuye Zhang Yuye Zhang Hongyu Kong Hongyu Kong Hongyu Kong Hongyu Kong Weiyi Xia Weiyi Xia Weiyi Xia Weiyi Xia Qinghe Jing Qinghe Jing Qinghe Jing Qinghe Jing Yuxiang Hu Yuxiang Hu Yuxiang Hu Yuxiang Hu Chen Zhao Chen Zhao Chen Zhao Chen Zhao Lianqun Wu Lianqun Wu Lianqun Wu Lianqun Wu Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis Frontiers in Cell and Developmental Biology thyroid-associated ophthalmopathy methylation orbital adipose/connective tissue methylation array pathology |
| title | Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis |
| title_full | Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis |
| title_fullStr | Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis |
| title_full_unstemmed | Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis |
| title_short | Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis |
| title_sort | adipose connective tissue from thyroid associated ophthalmopathy uncovers interdependence between methylation and disease pathogenesis a genome wide methylation analysis |
| topic | thyroid-associated ophthalmopathy methylation orbital adipose/connective tissue methylation array pathology |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.716871/full |
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