Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper
The diagnostic value of RT-PCR and hemi-nested RT-PCR (hnRT-PCR) was compared in brain samples of dogs presenting neurological signs compatible with canine distemper. Samples of central nervous system (CNS) were collected from 68 dogs and tested by direct immunofluorescence test (RFID) and, independ...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2015-12-01
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| Series: | Brazilian Journal of Veterinary Research and Animal Science |
| Subjects: | |
| Online Access: | http://www.revistas.usp.br/bjvras/article/view/88333 |
| _version_ | 1811266894502559744 |
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| author | Adriana Cortez Vanessa Souza Telma Fernandes Jane Megid |
| author_facet | Adriana Cortez Vanessa Souza Telma Fernandes Jane Megid |
| author_sort | Adriana Cortez |
| collection | DOAJ |
| description | The diagnostic value of RT-PCR and hemi-nested RT-PCR (hnRT-PCR) was compared in brain samples of dogs presenting neurological signs compatible with canine distemper. Samples of central nervous system (CNS) were collected from 68 dogs and tested by direct immunofluorescence test (RFID) and, independent of the results, they were stored at -20°C for at least three years. They were submitted to the RT-PCR and hnRT-PCR techniques aiming to determine the gene responsible for the viral nucleoprotein decoding. Fifty-nine samples were positive for RIFD, 40 for RT-PCR (Kappa = 0.358) and 54 for hnRT-PCR (Kappa = 0.740). All nine RIFD negative samples were also negative for RT-PCR and hnRT-PCR. In spite of the storage duration and proper sample conditions, the estimated accordance between hnRT-PCR and RIFD demonstrated that hnRT-PCR technique can be applied in retrospective studies. |
| first_indexed | 2024-04-12T20:51:43Z |
| format | Article |
| id | doaj.art-86008eb932424c639beba648f070c9ee |
| institution | Directory Open Access Journal |
| issn | 1413-9596 1678-4456 |
| language | English |
| last_indexed | 2024-04-12T20:51:43Z |
| publishDate | 2015-12-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Veterinary Research and Animal Science |
| spelling | doaj.art-86008eb932424c639beba648f070c9ee2022-12-22T03:17:06ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562015-12-0152410.11606/issn.1678-4456.v52i4p363-36596499Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemperAdriana Cortez0Vanessa Souza1Telma Fernandes2Jane Megid3Universidade de Santo Amaro, Medicina VeterináriaUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaThe diagnostic value of RT-PCR and hemi-nested RT-PCR (hnRT-PCR) was compared in brain samples of dogs presenting neurological signs compatible with canine distemper. Samples of central nervous system (CNS) were collected from 68 dogs and tested by direct immunofluorescence test (RFID) and, independent of the results, they were stored at -20°C for at least three years. They were submitted to the RT-PCR and hnRT-PCR techniques aiming to determine the gene responsible for the viral nucleoprotein decoding. Fifty-nine samples were positive for RIFD, 40 for RT-PCR (Kappa = 0.358) and 54 for hnRT-PCR (Kappa = 0.740). All nine RIFD negative samples were also negative for RT-PCR and hnRT-PCR. In spite of the storage duration and proper sample conditions, the estimated accordance between hnRT-PCR and RIFD demonstrated that hnRT-PCR technique can be applied in retrospective studies.http://www.revistas.usp.br/bjvras/article/view/88333Canine distemperhnRT-PCRDirect immunofluorescenceKappa index |
| spellingShingle | Adriana Cortez Vanessa Souza Telma Fernandes Jane Megid Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper Brazilian Journal of Veterinary Research and Animal Science Canine distemper hnRT-PCR Direct immunofluorescence Kappa index |
| title | Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper |
| title_full | Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper |
| title_fullStr | Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper |
| title_full_unstemmed | Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper |
| title_short | Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper |
| title_sort | evaluation of rt pcr and hemi nested rt pcr in brain samples from dogs with neurologic signs compatible with distemper |
| topic | Canine distemper hnRT-PCR Direct immunofluorescence Kappa index |
| url | http://www.revistas.usp.br/bjvras/article/view/88333 |
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