Transgenic mouse models enabling photolabeling of individual neurons in vivo.
One of the biggest tasks in neuroscience is to explain activity patterns of individual neurons during behavior by their cellular characteristics and their connectivity within the neuronal network. To greatly facilitate linking in vivo experiments with a more detailed molecular or physiological analy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3633923?pdf=render |
| _version_ | 1819238369834565632 |
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| author | Manuel Peter Brice Bathellier Bruno Fontinha Pinelopi Pliota Wulf Haubensak Simon Rumpel |
| author_facet | Manuel Peter Brice Bathellier Bruno Fontinha Pinelopi Pliota Wulf Haubensak Simon Rumpel |
| author_sort | Manuel Peter |
| collection | DOAJ |
| description | One of the biggest tasks in neuroscience is to explain activity patterns of individual neurons during behavior by their cellular characteristics and their connectivity within the neuronal network. To greatly facilitate linking in vivo experiments with a more detailed molecular or physiological analysis in vitro, we have generated and characterized genetically modified mice expressing photoactivatable GFP (PA-GFP) that allow conditional photolabeling of individual neurons. Repeated photolabeling at the soma reveals basic morphological features due to diffusion of activated PA-GFP into the dendrites. Neurons photolabeled in vivo can be re-identified in acute brain slices and targeted for electrophysiological recordings. We demonstrate the advantages of PA-GFP expressing mice by the correlation of in vivo firing rates of individual neurons with their expression levels of the immediate early gene c-fos. Generally, the mouse models described in this study enable the combination of various analytical approaches to characterize living cells, also beyond the neurosciences. |
| first_indexed | 2024-12-23T13:35:08Z |
| format | Article |
| id | doaj.art-8601851f50504f5697c5f4ce1490e8a0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T13:35:08Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8601851f50504f5697c5f4ce1490e8a02022-12-21T17:45:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6213210.1371/journal.pone.0062132Transgenic mouse models enabling photolabeling of individual neurons in vivo.Manuel PeterBrice BathellierBruno FontinhaPinelopi PliotaWulf HaubensakSimon RumpelOne of the biggest tasks in neuroscience is to explain activity patterns of individual neurons during behavior by their cellular characteristics and their connectivity within the neuronal network. To greatly facilitate linking in vivo experiments with a more detailed molecular or physiological analysis in vitro, we have generated and characterized genetically modified mice expressing photoactivatable GFP (PA-GFP) that allow conditional photolabeling of individual neurons. Repeated photolabeling at the soma reveals basic morphological features due to diffusion of activated PA-GFP into the dendrites. Neurons photolabeled in vivo can be re-identified in acute brain slices and targeted for electrophysiological recordings. We demonstrate the advantages of PA-GFP expressing mice by the correlation of in vivo firing rates of individual neurons with their expression levels of the immediate early gene c-fos. Generally, the mouse models described in this study enable the combination of various analytical approaches to characterize living cells, also beyond the neurosciences.http://europepmc.org/articles/PMC3633923?pdf=render |
| spellingShingle | Manuel Peter Brice Bathellier Bruno Fontinha Pinelopi Pliota Wulf Haubensak Simon Rumpel Transgenic mouse models enabling photolabeling of individual neurons in vivo. PLoS ONE |
| title | Transgenic mouse models enabling photolabeling of individual neurons in vivo. |
| title_full | Transgenic mouse models enabling photolabeling of individual neurons in vivo. |
| title_fullStr | Transgenic mouse models enabling photolabeling of individual neurons in vivo. |
| title_full_unstemmed | Transgenic mouse models enabling photolabeling of individual neurons in vivo. |
| title_short | Transgenic mouse models enabling photolabeling of individual neurons in vivo. |
| title_sort | transgenic mouse models enabling photolabeling of individual neurons in vivo |
| url | http://europepmc.org/articles/PMC3633923?pdf=render |
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