Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental Study
Objective: Physical exercise has different effects on oxidative stress. Oxidative stress influences TLR4 and NFkB gene expression. The purpose of this study was to investigate the effect of aerobic training and vitamin D on gene expression of TLR4 and NFkB in lung tissue of obese rats exposed to oxy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2020-03-01
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| Series: | Iranian Journal of Diabetes and Obesity |
| Subjects: | |
| Online Access: | http://ijdo.ssu.ac.ir/article-1-546-en.html |
| _version_ | 1819054968926109696 |
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| author | Milad Rahimi Mohammad Ali Azarbayjani Maghsoud Peeri Parvin Farzanegi |
| author_facet | Milad Rahimi Mohammad Ali Azarbayjani Maghsoud Peeri Parvin Farzanegi |
| author_sort | Milad Rahimi |
| collection | DOAJ |
| description | Objective: Physical exercise has different effects on oxidative stress. Oxidative stress influences TLR4 and NFkB gene expression. The purpose of this study was to investigate the effect of aerobic training and vitamin D on gene expression of TLR4 and NFkB in lung tissue of obese rats exposed to oxygenated water.
Materials and Methods: In an experimental study, 30 obese male wistar rats were randomly divided into five groups: control, oxygenated water, oxygenated water + vitamin D, oxygenated water + aerobic training, and oxygenated water + aerobic training + vitamin D. All the rats were injected intraperitoneally with oxygenated water. Vitamin D was performed by intraperitoneal injection of 0.5 μg daily for eight weeks. The aerobic training protocol included 8 weeks, 5 sessions per week running on treadmill. TLR4 and NFkB gene expression of lung tissue were investigated using real time & PCR. Two-way ANOVA and Bonferroni post hoc test were used to analysis the data. The significant level was set at P-value< 0.05.
Results: Aerobic training significantly reduced TLR4 expression compared with other groups (P-value: 0.046) but did not significantly affect the expression of NFkB gene (P-value: 0.261). Vitamin D alone and aerobic training and vitamin D interaction did not significantly alter the gene expression of TLR4 (P-value: 0.072 and P-value: 0.695, respectively) and NFkB (P-value: 0.243 and P-value:< 0.195, respectively).
Conclusion: It seems that performing aerobic training is likely to be beneficial in reducing oxidative stress and inflammation compared to inactivity. |
| first_indexed | 2024-12-21T13:00:03Z |
| format | Article |
| id | doaj.art-8601bef343f743f79de24722b442aec6 |
| institution | Directory Open Access Journal |
| issn | 2008-6792 2345-2250 |
| language | English |
| last_indexed | 2024-12-21T13:00:03Z |
| publishDate | 2020-03-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Diabetes and Obesity |
| spelling | doaj.art-8601bef343f743f79de24722b442aec62022-12-21T19:03:13ZengShahid Sadoughi University of Medical SciencesIranian Journal of Diabetes and Obesity2008-67922345-22502020-03-011213036Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental StudyMilad Rahimi0Mohammad Ali Azarbayjani1Maghsoud Peeri2Parvin Farzanegi3 PHD Candidate, Department of Exercise Physiology, Islamic Azad University, Central Branch, Tehran, Iran. Full Professor, Department of Exercise Physiology, Islamic Azad University, Central Branch, Tehran, Iran. Full Professor, Department of Exercise Physiology, Islamic Azad University, Central Branch, Tehran, Iran. Associate Professor, Department of Exercise Physiology, Islamic Azad University, Sari Branch, Mazandaran, Iran. Objective: Physical exercise has different effects on oxidative stress. Oxidative stress influences TLR4 and NFkB gene expression. The purpose of this study was to investigate the effect of aerobic training and vitamin D on gene expression of TLR4 and NFkB in lung tissue of obese rats exposed to oxygenated water. Materials and Methods: In an experimental study, 30 obese male wistar rats were randomly divided into five groups: control, oxygenated water, oxygenated water + vitamin D, oxygenated water + aerobic training, and oxygenated water + aerobic training + vitamin D. All the rats were injected intraperitoneally with oxygenated water. Vitamin D was performed by intraperitoneal injection of 0.5 μg daily for eight weeks. The aerobic training protocol included 8 weeks, 5 sessions per week running on treadmill. TLR4 and NFkB gene expression of lung tissue were investigated using real time & PCR. Two-way ANOVA and Bonferroni post hoc test were used to analysis the data. The significant level was set at P-value< 0.05. Results: Aerobic training significantly reduced TLR4 expression compared with other groups (P-value: 0.046) but did not significantly affect the expression of NFkB gene (P-value: 0.261). Vitamin D alone and aerobic training and vitamin D interaction did not significantly alter the gene expression of TLR4 (P-value: 0.072 and P-value: 0.695, respectively) and NFkB (P-value: 0.243 and P-value:< 0.195, respectively). Conclusion: It seems that performing aerobic training is likely to be beneficial in reducing oxidative stress and inflammation compared to inactivity.http://ijdo.ssu.ac.ir/article-1-546-en.htmlaerobic trainingoxidative stresstlr4nfkb |
| spellingShingle | Milad Rahimi Mohammad Ali Azarbayjani Maghsoud Peeri Parvin Farzanegi Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental Study Iranian Journal of Diabetes and Obesity aerobic training oxidative stress tlr4 nfkb |
| title | Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental Study |
| title_full | Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental Study |
| title_fullStr | Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental Study |
| title_full_unstemmed | Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental Study |
| title_short | Vitamin D and Training Modulates Gene Expression of TLR4 and NFkB in Lung Tissue of Obese Rats in an Experimental Study |
| title_sort | vitamin d and training modulates gene expression of tlr4 and nfkb in lung tissue of obese rats in an experimental study |
| topic | aerobic training oxidative stress tlr4 nfkb |
| url | http://ijdo.ssu.ac.ir/article-1-546-en.html |
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