Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus
Efficient propagation of Chang Daeng through embryogenic callus was investigated. Young shoot (approx. 10 mm)gave the highest percentage of multiple shoot formation (100), number of shoots (10 shoots/explant) and embryogenic callus(EC) formation (56%) Proliferation of the calli in both Vacin and Wen...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2011-12-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjstweb/journal/33-6/0125-3395-33-6-659-663.pdf |
| _version_ | 1818063085291700224 |
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| author | Suphat Rittirat1 Sompong Te-chato2* Soisiri Kongruk2 |
| author_facet | Suphat Rittirat1 Sompong Te-chato2* Soisiri Kongruk2 |
| author_sort | Suphat Rittirat1 |
| collection | DOAJ |
| description | Efficient propagation of Chang Daeng through embryogenic callus was investigated. Young shoot (approx. 10 mm)gave the highest percentage of multiple shoot formation (100), number of shoots (10 shoots/explant) and embryogenic callus(EC) formation (56%) Proliferation of the calli in both Vacin and Went (VW) and New Dogashima medium (NDM) supplementedwith 2% sucrose and 15% coconut water (CW) under light or dark condition was nearly the same. The color of calliin VW in both conditions was light yellow or cream while NDM provided bright yellow or yellowish green. Calli subculturedon NDM medium under light condition turned to green and then produced the highest percentage formation (79.8) andnumber of mature somatic embryos (9 embryos/callus). Within one month, those embryos germinated in the presence of ACin the culture medium. The shoots at length more than 3 cm with an average number of 2-3 roots were successfully transferredto soil. This empirical technique could be useful for micropropagation as well as genetic transformation in this plant. |
| first_indexed | 2024-12-10T14:14:30Z |
| format | Article |
| id | doaj.art-860282ade60f4eaf8ca44294145f9b6f |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-10T14:14:30Z |
| publishDate | 2011-12-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-860282ade60f4eaf8ca44294145f9b6f2022-12-22T01:45:23ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952011-12-01336659663Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callusSuphat Rittirat1Sompong Te-chato2*Soisiri Kongruk2Efficient propagation of Chang Daeng through embryogenic callus was investigated. Young shoot (approx. 10 mm)gave the highest percentage of multiple shoot formation (100), number of shoots (10 shoots/explant) and embryogenic callus(EC) formation (56%) Proliferation of the calli in both Vacin and Went (VW) and New Dogashima medium (NDM) supplementedwith 2% sucrose and 15% coconut water (CW) under light or dark condition was nearly the same. The color of calliin VW in both conditions was light yellow or cream while NDM provided bright yellow or yellowish green. Calli subculturedon NDM medium under light condition turned to green and then produced the highest percentage formation (79.8) andnumber of mature somatic embryos (9 embryos/callus). Within one month, those embryos germinated in the presence of ACin the culture medium. The shoots at length more than 3 cm with an average number of 2-3 roots were successfully transferredto soil. This empirical technique could be useful for micropropagation as well as genetic transformation in this plant.http://rdo.psu.ac.th/sjstweb/journal/33-6/0125-3395-33-6-659-663.pdfChang DaengRhynchostylis rubrummicropropagationembryogenic callus |
| spellingShingle | Suphat Rittirat1 Sompong Te-chato2* Soisiri Kongruk2 Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus Songklanakarin Journal of Science and Technology (SJST) Chang Daeng Rhynchostylis rubrum micropropagation embryogenic callus |
| title | Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus |
| title_full | Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus |
| title_fullStr | Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus |
| title_full_unstemmed | Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus |
| title_short | Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus |
| title_sort | micropropagation of chang daeng rhynchostylis gigantea var sagarik by embryogenic callus |
| topic | Chang Daeng Rhynchostylis rubrum micropropagation embryogenic callus |
| url | http://rdo.psu.ac.th/sjstweb/journal/33-6/0125-3395-33-6-659-663.pdf |
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