Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding
Both matrix biochemistry and neurotrophic factors are known to modulate neurite outgrowth and pathfinding however, the interplay between these two factors is less studied. While previous work has shown that the biochemical identity of the matrix can alter the outgrowth of neurites in response to neu...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=4;spage=514;epage=517;aulast=Madl |
| _version_ | 1818305851052523520 |
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| author | Christopher M Madl Sarah C Heilshorn |
| author_facet | Christopher M Madl Sarah C Heilshorn |
| author_sort | Christopher M Madl |
| collection | DOAJ |
| description | Both matrix biochemistry and neurotrophic factors are known to modulate neurite outgrowth and pathfinding however, the interplay between these two factors is less studied. While previous work has shown that the biochemical identity of the matrix can alter the outgrowth of neurites in response to neurotrophins, the importance of the concentration of cell-adhesive ligands is unknown. Using engineered elastin-like protein matrices, we recently demonstrated a synergistic effect between matrix-bound cell-adhesive ligand density and soluble nerve growth factor treatment on neurite outgrowth from dorsal root ganglia. This synergism was mediated by Schwann cell-neurite contact through L1CAM. Cell-adhesive ligand density was also shown to alter the pathfinding behavior of dorsal root ganglion neurites in response to a gradient of nerve growth factor. While more cell-adhesive matrices promoted neurite outgrowth, less cell-adhesive matrices promoted more faithful neurite pathfinding. These studies emphasize the importance of considering both matrix biochemistry and neurotrophic factors when designing biomaterials for peripheral nerve regeneration. |
| first_indexed | 2024-12-13T06:33:09Z |
| format | Article |
| id | doaj.art-8603c58a188f434e9096cea3007cf8d8 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-13T06:33:09Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-8603c58a188f434e9096cea3007cf8d82022-12-21T23:56:33ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742015-01-0110451451710.4103/1673-5374.155426Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfindingChristopher M MadlSarah C HeilshornBoth matrix biochemistry and neurotrophic factors are known to modulate neurite outgrowth and pathfinding however, the interplay between these two factors is less studied. While previous work has shown that the biochemical identity of the matrix can alter the outgrowth of neurites in response to neurotrophins, the importance of the concentration of cell-adhesive ligands is unknown. Using engineered elastin-like protein matrices, we recently demonstrated a synergistic effect between matrix-bound cell-adhesive ligand density and soluble nerve growth factor treatment on neurite outgrowth from dorsal root ganglia. This synergism was mediated by Schwann cell-neurite contact through L1CAM. Cell-adhesive ligand density was also shown to alter the pathfinding behavior of dorsal root ganglion neurites in response to a gradient of nerve growth factor. While more cell-adhesive matrices promoted neurite outgrowth, less cell-adhesive matrices promoted more faithful neurite pathfinding. These studies emphasize the importance of considering both matrix biochemistry and neurotrophic factors when designing biomaterials for peripheral nerve regeneration.http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=4;spage=514;epage=517;aulast=Madlspinal cord injurypropriospinal systemneural plasticityfiber sproutingneural repaircompensationregenerationpropriospinal detoursneurotrophic factorscell-adhesive ligandsdorsal root gangliaL1CAMnerve growth factorbiomaterialselastin-like proteins |
| spellingShingle | Christopher M Madl Sarah C Heilshorn Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding Neural Regeneration Research spinal cord injury propriospinal system neural plasticity fiber sprouting neural repair compensation regeneration propriospinal detours neurotrophic factors cell-adhesive ligands dorsal root ganglia L1CAM nerve growth factor biomaterials elastin-like proteins |
| title | Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding |
| title_full | Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding |
| title_fullStr | Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding |
| title_full_unstemmed | Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding |
| title_short | Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding |
| title_sort | matrix interactions modulate neurotrophin mediated neurite outgrowth and pathfinding |
| topic | spinal cord injury propriospinal system neural plasticity fiber sprouting neural repair compensation regeneration propriospinal detours neurotrophic factors cell-adhesive ligands dorsal root ganglia L1CAM nerve growth factor biomaterials elastin-like proteins |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=4;spage=514;epage=517;aulast=Madl |
| work_keys_str_mv | AT christophermmadl matrixinteractionsmodulateneurotrophinmediatedneuriteoutgrowthandpathfinding AT sarahcheilshorn matrixinteractionsmodulateneurotrophinmediatedneuriteoutgrowthandpathfinding |