Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect
Designing therapeutic and sensor materials to diagnose and eliminate bacterial infections remains a significant challenge for active theragnostic nanoprobes. In the present work, fluorescent/electroactive poly(3-hexylthiophene) P3HT nanoparticles (NPs) stabilized with quaternary ammonium salts using...
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MDPI AG
2021-03-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/5/1715 |
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| author | Nada Elgiddawy Shiwei Ren Wadih Ghattas Waleed M. A. El Rouby Ahmed O. El-Gendy Ahmed A. Farghali Abderrahim Yassar Hafsa Korri-Youssoufi |
| author_facet | Nada Elgiddawy Shiwei Ren Wadih Ghattas Waleed M. A. El Rouby Ahmed O. El-Gendy Ahmed A. Farghali Abderrahim Yassar Hafsa Korri-Youssoufi |
| author_sort | Nada Elgiddawy |
| collection | DOAJ |
| description | Designing therapeutic and sensor materials to diagnose and eliminate bacterial infections remains a significant challenge for active theragnostic nanoprobes. In the present work, fluorescent/electroactive poly(3-hexylthiophene) P3HT nanoparticles (NPs) stabilized with quaternary ammonium salts using cetyltrimethylammonium bromide (CTAB), (CTAB-P3HT NPs) were prepared using a simple mini-emulsion method. The morphology, spectroscopic properties and electronic properties of CTAB-P3HT NPs were characterized by DLS, zeta potential, SEM, TEM, UV-vis spectrophotometry, fluorescence spectroscopy and electrochemical impedance spectroscopy (EIS). In an aqueous solution, CTAB-P3HT NPs were revealed to be uniformly sized, highly fluorescent and present a highly positively charged NP surface with good electroactivity. Dual detection was demonstrated as the binding of the bacteria to NPs could be observed by fluorescence quenching as well as by the changes in EIS. Binding of <i>E. coli</i> to CTAB-P3HT NPs was demonstrated and LODs of 5 CFU/mL and 250 CFU/mL were obtained by relying on the fluorescence spectroscopy and EIS, respectively. The antimicrobial activity of CTAB-P3HT NPs on bacteria and fungi was also studied under dark and nutritive conditions. An MIC and an MBC of 2.5 µg/mL were obtained with <i>E. coli</i> and with <i>S. aureus</i>, and of 0.312 µg/mL with <i>C. albicans</i>. Additionally a good biocompatibility toward normal human cells (WI38) was observed, which opens the way to their possible use as a therapeutic agent. |
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| language | English |
| last_indexed | 2024-03-09T05:57:23Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-860b38dc05bf4876bf2a4c941327fa6c2023-12-03T12:12:29ZengMDPI AGSensors1424-82202021-03-01215171510.3390/s21051715Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic ProspectNada Elgiddawy0Shiwei Ren1Wadih Ghattas2Waleed M. A. El Rouby3Ahmed O. El-Gendy4Ahmed A. Farghali5Abderrahim Yassar6Hafsa Korri-Youssoufi7Université Paris-Saclay, CNRS, Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), ECBB, 91400 Orsay, FranceLPICM, CNRS, Ecole Polytechnique, Institut Polytechnique de Paris, Route de Saclay, 91128 Palaiseau, FranceUniversité Paris-Saclay, CNRS, Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), ECBB, 91400 Orsay, FranceMaterials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef 62 511, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, EgyptMaterials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef 62 511, EgyptLPICM, CNRS, Ecole Polytechnique, Institut Polytechnique de Paris, Route de Saclay, 91128 Palaiseau, FranceUniversité Paris-Saclay, CNRS, Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), ECBB, 91400 Orsay, FranceDesigning therapeutic and sensor materials to diagnose and eliminate bacterial infections remains a significant challenge for active theragnostic nanoprobes. In the present work, fluorescent/electroactive poly(3-hexylthiophene) P3HT nanoparticles (NPs) stabilized with quaternary ammonium salts using cetyltrimethylammonium bromide (CTAB), (CTAB-P3HT NPs) were prepared using a simple mini-emulsion method. The morphology, spectroscopic properties and electronic properties of CTAB-P3HT NPs were characterized by DLS, zeta potential, SEM, TEM, UV-vis spectrophotometry, fluorescence spectroscopy and electrochemical impedance spectroscopy (EIS). In an aqueous solution, CTAB-P3HT NPs were revealed to be uniformly sized, highly fluorescent and present a highly positively charged NP surface with good electroactivity. Dual detection was demonstrated as the binding of the bacteria to NPs could be observed by fluorescence quenching as well as by the changes in EIS. Binding of <i>E. coli</i> to CTAB-P3HT NPs was demonstrated and LODs of 5 CFU/mL and 250 CFU/mL were obtained by relying on the fluorescence spectroscopy and EIS, respectively. The antimicrobial activity of CTAB-P3HT NPs on bacteria and fungi was also studied under dark and nutritive conditions. An MIC and an MBC of 2.5 µg/mL were obtained with <i>E. coli</i> and with <i>S. aureus</i>, and of 0.312 µg/mL with <i>C. albicans</i>. Additionally a good biocompatibility toward normal human cells (WI38) was observed, which opens the way to their possible use as a therapeutic agent.https://www.mdpi.com/1424-8220/21/5/1715cationic conjugated-polymersnanoparticlesmini-emulsionfluorescenceimpedancedetection |
| spellingShingle | Nada Elgiddawy Shiwei Ren Wadih Ghattas Waleed M. A. El Rouby Ahmed O. El-Gendy Ahmed A. Farghali Abderrahim Yassar Hafsa Korri-Youssoufi Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect Sensors cationic conjugated-polymers nanoparticles mini-emulsion fluorescence impedance detection |
| title | Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect |
| title_full | Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect |
| title_fullStr | Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect |
| title_full_unstemmed | Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect |
| title_short | Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect |
| title_sort | antimicrobial activity of cationic poly 3 hexylthiophene nanoparticles coupled with dual fluorescent and electrochemical sensing theragnostic prospect |
| topic | cationic conjugated-polymers nanoparticles mini-emulsion fluorescence impedance detection |
| url | https://www.mdpi.com/1424-8220/21/5/1715 |
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