Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor
The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was f...
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MDPI AG
2018-11-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/18/11/4032 |
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| author | Dae Hoon Kim Hong Gi Oh Woo Hwan Park Dong Cheol Jeon Ki Moo Lim Hyung Jin Kim Byoung Kuk Jang Kwang Soup Song |
| author_facet | Dae Hoon Kim Hong Gi Oh Woo Hwan Park Dong Cheol Jeon Ki Moo Lim Hyung Jin Kim Byoung Kuk Jang Kwang Soup Song |
| author_sort | Dae Hoon Kim |
| collection | DOAJ |
| description | The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid <i>N</i>-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (ΔV<sub>Dirac</sub>) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL<sup>−1</sup> in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL<sup>−1</sup>, with a detection sensitivity of 5.68 mV. The sensitivity (ΔV<sub>Dirac</sub>) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics. |
| first_indexed | 2024-04-11T21:44:01Z |
| format | Article |
| id | doaj.art-860c7e09b69142f8a80d5beee1997619 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T21:44:01Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-860c7e09b69142f8a80d5beee19976192022-12-22T04:01:28ZengMDPI AGSensors1424-82202018-11-011811403210.3390/s18114032s18114032Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect TransistorDae Hoon Kim0Hong Gi Oh1Woo Hwan Park2Dong Cheol Jeon3Ki Moo Lim4Hyung Jin Kim5Byoung Kuk Jang6Kwang Soup Song7Department of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, KoreaDepartment of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, KoreaDepartment of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, KoreaDepartment of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, KoreaDepartment of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, KoreaBiomedical IT Convergence Center, Gumi Electronics and Information Technology Research Institute, Gumi, Gyeongbuk 39171, KoreaDepartment of Internal Medicine, Keimyung University School of Medicine, Daegu 41931, KoreaDepartment of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, KoreaThe detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid <i>N</i>-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (ΔV<sub>Dirac</sub>) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL<sup>−1</sup> in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL<sup>−1</sup>, with a detection sensitivity of 5.68 mV. The sensitivity (ΔV<sub>Dirac</sub>) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics.https://www.mdpi.com/1424-8220/18/11/4032hepatocellular carcinomaalpha-fetoproteinbiosensorgraphenefield-effect transistor |
| spellingShingle | Dae Hoon Kim Hong Gi Oh Woo Hwan Park Dong Cheol Jeon Ki Moo Lim Hyung Jin Kim Byoung Kuk Jang Kwang Soup Song Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor Sensors hepatocellular carcinoma alpha-fetoprotein biosensor graphene field-effect transistor |
| title | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
| title_full | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
| title_fullStr | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
| title_full_unstemmed | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
| title_short | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
| title_sort | detection of alpha fetoprotein in hepatocellular carcinoma patient plasma with graphene field effect transistor |
| topic | hepatocellular carcinoma alpha-fetoprotein biosensor graphene field-effect transistor |
| url | https://www.mdpi.com/1424-8220/18/11/4032 |
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