Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains
Introduction Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2019-03-01
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| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/aaem/EVALUATION-OF-IN-VITRO-EFFECT-OF-SELECTED-CONTACT-LENS-SOLUTIONS-CONJUGATED-WITH,105394,0,2.html |
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| author | Marcin Padzik Edyta B. Hendiger Agnieszka Żochowska Jarosław Szczepaniak Wanda Baltaza Anna Pietruczuk-Padzik Gabriela Olędzka Lidia Chomicz |
| author_facet | Marcin Padzik Edyta B. Hendiger Agnieszka Żochowska Jarosław Szczepaniak Wanda Baltaza Anna Pietruczuk-Padzik Gabriela Olędzka Lidia Chomicz |
| author_sort | Marcin Padzik |
| collection | DOAJ |
| description | Introduction
Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites.
Material and methods
Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line.
Results
Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed.
Conclusions
Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users. |
| first_indexed | 2024-12-19T19:30:52Z |
| format | Article |
| id | doaj.art-8610f91d1b7a474db049eab584b25e2b |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-19T19:30:52Z |
| publishDate | 2019-03-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-8610f91d1b7a474db049eab584b25e2b2022-12-21T20:08:36ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632019-03-0126119820210.26444/aaem/105394105394Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strainsMarcin Padzik0Edyta B. Hendiger1Agnieszka Żochowska2Jarosław Szczepaniak3Wanda Baltaza4Anna Pietruczuk-Padzik5Gabriela Olędzka6Lidia Chomicz7Department of Medical Biology, Medical University, Warsaw, PolandDepartment of Medical Biology, Medical University, Warsaw, PolandDepartment of Medical Biology, Medical University, Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, University of Life Science, Warsaw, PolandDepartment of Medical Biology, Medical University, Warsaw, PolandDepartment of Pharmaceutical Microbiology, Medical University, Warsaw, PolandDepartment of Medical Biology, Medical University, Warsaw, PolandDepartment of Medical Biology, Medical University, Warsaw, PolandIntroduction Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. Material and methods Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Results Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Conclusions Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.http://www.journalssystem.com/aaem/EVALUATION-OF-IN-VITRO-EFFECT-OF-SELECTED-CONTACT-LENS-SOLUTIONS-CONJUGATED-WITH,105394,0,2.htmlAcanthamoeba keratitiscontact lens solutionssilver/gold nanoparticlesAcanthamoeba trophozoitesanti-amoebic in vitro effect |
| spellingShingle | Marcin Padzik Edyta B. Hendiger Agnieszka Żochowska Jarosław Szczepaniak Wanda Baltaza Anna Pietruczuk-Padzik Gabriela Olędzka Lidia Chomicz Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains Annals of Agricultural and Environmental Medicine Acanthamoeba keratitis contact lens solutions silver/gold nanoparticles Acanthamoeba trophozoites anti-amoebic in vitro effect |
| title | Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains |
| title_full | Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains |
| title_fullStr | Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains |
| title_full_unstemmed | Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains |
| title_short | Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains |
| title_sort | evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by acanthamoeba strains |
| topic | Acanthamoeba keratitis contact lens solutions silver/gold nanoparticles Acanthamoeba trophozoites anti-amoebic in vitro effect |
| url | http://www.journalssystem.com/aaem/EVALUATION-OF-IN-VITRO-EFFECT-OF-SELECTED-CONTACT-LENS-SOLUTIONS-CONJUGATED-WITH,105394,0,2.html |
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