Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism
Abstract Oilseed rape (Brassica napus) is an important crop that is cultivated for the oil (mainly triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key enzymes in the Kennedy pathway, such as glycerol 3-phosphate acyltransferase (GPAT), lysophosphatidate acyltran...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-07387-x |
| _version_ | 1830354026206593024 |
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| author | Pan Liao Tamara Lechon Trevor Romsdahl Helen Woodfield Stepan Fenyk Tony Fawcett Emma Wallington Ruth E. Bates Mee-Len Chye Kent D. Chapman John L. Harwood Simon Scofield |
| author_facet | Pan Liao Tamara Lechon Trevor Romsdahl Helen Woodfield Stepan Fenyk Tony Fawcett Emma Wallington Ruth E. Bates Mee-Len Chye Kent D. Chapman John L. Harwood Simon Scofield |
| author_sort | Pan Liao |
| collection | DOAJ |
| description | Abstract Oilseed rape (Brassica napus) is an important crop that is cultivated for the oil (mainly triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key enzymes in the Kennedy pathway, such as glycerol 3-phosphate acyltransferase (GPAT), lysophosphatidate acyltransferase (LPAT) and diacylglycerol acyltransferase (DGAT) but can also be produced from phosphoglycerides such as phosphatidylcholine (PC) by the activity of the enzyme phospholipid: diacylglycerol acyltransferase (PDAT). To evaluate the potential for these enzymes to alter oil yields or composition, we analysed transgenic B. napus lines which overexpressed GPAT, LPAT or PDAT using heterologous transgenes from Arabidopsis and Nasturtium and examined lipid profiles and changes in gene expression in these lines compared to WT. Distinct changes in PC and TAG abundance and spatial distribution in embryonic tissues were observed in some of the transgenic lines, together with altered expression of genes involved generally in acyl-lipid metabolism. Overall our results show that up-regulation of these key enzymes differentially affects lipid composition and distribution as well as lipid-associated gene expression, providing important information which could be used to improve crop properties by metabolic engineering. |
| first_indexed | 2024-12-20T01:24:43Z |
| format | Article |
| id | doaj.art-86111c824ec5497fb46a2a3d177b3cd8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T01:24:43Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-86111c824ec5497fb46a2a3d177b3cd82022-12-21T19:58:17ZengNature PortfolioScientific Reports2045-23222022-03-0112111410.1038/s41598-022-07387-xTransgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolismPan Liao0Tamara Lechon1Trevor Romsdahl2Helen Woodfield3Stepan Fenyk4Tony Fawcett5Emma Wallington6Ruth E. Bates7Mee-Len Chye8Kent D. Chapman9John L. Harwood10Simon Scofield11School of Biological Science, University of Hong KongSchool of Biosciences, Cardiff UniversityDepartment of Biological Sciences, BioDiscovery Institute, University of North TexasSchool of Biosciences, Cardiff UniversityDepartment of Biosciences, Durham UniversityDepartment of Biosciences, Durham UniversityJohn Bingham Lab., National Institute for Agricultural BotanyJohn Bingham Lab., National Institute for Agricultural BotanySchool of Biological Science, University of Hong KongDepartment of Biological Sciences, BioDiscovery Institute, University of North TexasSchool of Biosciences, Cardiff UniversitySchool of Biosciences, Cardiff UniversityAbstract Oilseed rape (Brassica napus) is an important crop that is cultivated for the oil (mainly triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key enzymes in the Kennedy pathway, such as glycerol 3-phosphate acyltransferase (GPAT), lysophosphatidate acyltransferase (LPAT) and diacylglycerol acyltransferase (DGAT) but can also be produced from phosphoglycerides such as phosphatidylcholine (PC) by the activity of the enzyme phospholipid: diacylglycerol acyltransferase (PDAT). To evaluate the potential for these enzymes to alter oil yields or composition, we analysed transgenic B. napus lines which overexpressed GPAT, LPAT or PDAT using heterologous transgenes from Arabidopsis and Nasturtium and examined lipid profiles and changes in gene expression in these lines compared to WT. Distinct changes in PC and TAG abundance and spatial distribution in embryonic tissues were observed in some of the transgenic lines, together with altered expression of genes involved generally in acyl-lipid metabolism. Overall our results show that up-regulation of these key enzymes differentially affects lipid composition and distribution as well as lipid-associated gene expression, providing important information which could be used to improve crop properties by metabolic engineering.https://doi.org/10.1038/s41598-022-07387-x |
| spellingShingle | Pan Liao Tamara Lechon Trevor Romsdahl Helen Woodfield Stepan Fenyk Tony Fawcett Emma Wallington Ruth E. Bates Mee-Len Chye Kent D. Chapman John L. Harwood Simon Scofield Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism Scientific Reports |
| title | Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism |
| title_full | Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism |
| title_fullStr | Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism |
| title_full_unstemmed | Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism |
| title_short | Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism |
| title_sort | transgenic manipulation of triacylglycerol biosynthetic enzymes in b napus alters lipid associated gene expression and lipid metabolism |
| url | https://doi.org/10.1038/s41598-022-07387-x |
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