An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to Indole
<i>Helicoverpa armigera</i> is a serious agricultural pest with polyphagous diets, widespread distribution, and causing severe damage. Among sixty-five candidate ORs in <i>H. armigera</i>, the co-receptor HarmOrco and three specific ORs with partial sequences were identified...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/4/385 |
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| author | Mengbo Guo Xueting Ren Yang Liu Guirong Wang |
| author_facet | Mengbo Guo Xueting Ren Yang Liu Guirong Wang |
| author_sort | Mengbo Guo |
| collection | DOAJ |
| description | <i>Helicoverpa armigera</i> is a serious agricultural pest with polyphagous diets, widespread distribution, and causing severe damage. Among sixty-five candidate ORs in <i>H. armigera</i>, the co-receptor HarmOrco and three specific ORs with partial sequences were identified to be expressed in the proboscis by our previous work, whereas their exact function is not known yet. In this study, we first confirmed the expression of these ORs in the proboscis by full-length cloning, which obtained the complete coding region of HarmOrco, OR24, and OR30. We then performed functional identification of HarmOR24 and OR30 by co-expressing them respectively with HarmOrco in <i>Xenopus</i> oocytes eukaryotic expression system combined with two-electrode voltage-clamp physiology. By testing the response of HarmOR24/OR30-expressing oocytes against eighty structural-divergent compounds, respectively, HarmOR30 was characterized to narrowly tune to indole and showed a specific tuning spectrum compared to its ortholog in <i>Spodoptera littoralis</i>. As indole is a distinctive herbivore-induced plant volatile and floral scent component, HarmOR30 might play roles in foraging and mediating the interactions between <i>H. armigera</i> with its surrounding environment. |
| first_indexed | 2024-03-09T04:34:02Z |
| format | Article |
| id | doaj.art-8614889280004a2ea85d3cc58efdb318 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T04:34:02Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-8614889280004a2ea85d3cc58efdb3182023-12-03T13:32:18ZengMDPI AGInsects2075-44502022-04-0113438510.3390/insects13040385An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to IndoleMengbo Guo0Xueting Ren1Yang Liu2Guirong Wang3Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaShenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China<i>Helicoverpa armigera</i> is a serious agricultural pest with polyphagous diets, widespread distribution, and causing severe damage. Among sixty-five candidate ORs in <i>H. armigera</i>, the co-receptor HarmOrco and three specific ORs with partial sequences were identified to be expressed in the proboscis by our previous work, whereas their exact function is not known yet. In this study, we first confirmed the expression of these ORs in the proboscis by full-length cloning, which obtained the complete coding region of HarmOrco, OR24, and OR30. We then performed functional identification of HarmOR24 and OR30 by co-expressing them respectively with HarmOrco in <i>Xenopus</i> oocytes eukaryotic expression system combined with two-electrode voltage-clamp physiology. By testing the response of HarmOR24/OR30-expressing oocytes against eighty structural-divergent compounds, respectively, HarmOR30 was characterized to narrowly tune to indole and showed a specific tuning spectrum compared to its ortholog in <i>Spodoptera littoralis</i>. As indole is a distinctive herbivore-induced plant volatile and floral scent component, HarmOR30 might play roles in foraging and mediating the interactions between <i>H. armigera</i> with its surrounding environment.https://www.mdpi.com/2075-4450/13/4/385odorant receptorproboscis<i>Helicoverpa armigera</i>functional identificationindole |
| spellingShingle | Mengbo Guo Xueting Ren Yang Liu Guirong Wang An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to Indole Insects odorant receptor proboscis <i>Helicoverpa armigera</i> functional identification indole |
| title | An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to Indole |
| title_full | An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to Indole |
| title_fullStr | An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to Indole |
| title_full_unstemmed | An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to Indole |
| title_short | An Odorant Receptor from the Proboscis of the Cotton Bollworm <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae) Narrowly Tuned to Indole |
| title_sort | odorant receptor from the proboscis of the cotton bollworm i helicoverpa armigera i lepidoptera noctuidae narrowly tuned to indole |
| topic | odorant receptor proboscis <i>Helicoverpa armigera</i> functional identification indole |
| url | https://www.mdpi.com/2075-4450/13/4/385 |
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