Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus
Multiple sclerosis (MS) often leads to the development of neurogenic lower urinary tract symptoms (LUTS). We previously characterized neurogenic bladder dysfunction in a mouse model of MS induced by a coronavirus, mouse hepatitis virus (MHV). The aim of the study was to identify genes and pathways l...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733897/?tool=EBI |
| _version_ | 1798006256693149696 |
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| author | Taylor C. Clarkson Nao Iguchi Alison Xiaoqiao Xie Anna P. Malykhina |
| author_facet | Taylor C. Clarkson Nao Iguchi Alison Xiaoqiao Xie Anna P. Malykhina |
| author_sort | Taylor C. Clarkson |
| collection | DOAJ |
| description | Multiple sclerosis (MS) often leads to the development of neurogenic lower urinary tract symptoms (LUTS). We previously characterized neurogenic bladder dysfunction in a mouse model of MS induced by a coronavirus, mouse hepatitis virus (MHV). The aim of the study was to identify genes and pathways linking neuroinflammation in the central nervous system with urinary bladder (UB) dysfunction to enhance our understanding of the mechanisms underlying LUTS in demyelinating diseases. Adult C57BL/6 male mice (N = 12) received either an intracranial injection of MHV (coronavirus-induced encephalomyelitis, CIE group), or sterile saline (control group). Spinal cord (SC) and urinary bladders (UB) were collected from CIE mice at 1 wk and 4 wks, followed by RNA isolation and NanoString nCounter Neuroinflammation assay. Transcriptome analysis of SC identified a significantly changed expression of >150 genes in CIE mice known to regulate astrocyte, microglia and oligodendrocyte functions, neuroinflammation and immune responses. Two genes were significantly upregulated (Ttr and Ms4a4a), and two were downregulated (Asb2 and Myct1) only in the UB of CIE mice. Siglec1 and Zbp1 were the only genes significantly upregulated in both tissues, suggesting a common transcriptomic link between neuroinflammation in the CNS and neurogenic changes in the UB of CIE mice. |
| first_indexed | 2024-04-11T12:51:57Z |
| format | Article |
| id | doaj.art-86171a9418c543c29f8a311a5be913d5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T12:51:57Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-86171a9418c543c29f8a311a5be913d52022-12-22T04:23:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirusTaylor C. ClarksonNao IguchiAlison Xiaoqiao XieAnna P. MalykhinaMultiple sclerosis (MS) often leads to the development of neurogenic lower urinary tract symptoms (LUTS). We previously characterized neurogenic bladder dysfunction in a mouse model of MS induced by a coronavirus, mouse hepatitis virus (MHV). The aim of the study was to identify genes and pathways linking neuroinflammation in the central nervous system with urinary bladder (UB) dysfunction to enhance our understanding of the mechanisms underlying LUTS in demyelinating diseases. Adult C57BL/6 male mice (N = 12) received either an intracranial injection of MHV (coronavirus-induced encephalomyelitis, CIE group), or sterile saline (control group). Spinal cord (SC) and urinary bladders (UB) were collected from CIE mice at 1 wk and 4 wks, followed by RNA isolation and NanoString nCounter Neuroinflammation assay. Transcriptome analysis of SC identified a significantly changed expression of >150 genes in CIE mice known to regulate astrocyte, microglia and oligodendrocyte functions, neuroinflammation and immune responses. Two genes were significantly upregulated (Ttr and Ms4a4a), and two were downregulated (Asb2 and Myct1) only in the UB of CIE mice. Siglec1 and Zbp1 were the only genes significantly upregulated in both tissues, suggesting a common transcriptomic link between neuroinflammation in the CNS and neurogenic changes in the UB of CIE mice.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733897/?tool=EBI |
| spellingShingle | Taylor C. Clarkson Nao Iguchi Alison Xiaoqiao Xie Anna P. Malykhina Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus PLoS ONE |
| title | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
| title_full | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
| title_fullStr | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
| title_full_unstemmed | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
| title_short | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
| title_sort | differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733897/?tool=EBI |
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