Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties
An efficient route for the synthesis of the phosphoramidite derivative of 5-methylcytosine bearing a tert-butylsulfanyl group protected thiol is described. This building block is used for the preparation of oligonucleotides carrying a thiol group at the nucleobase at the internal position of a DNA s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/15/8/5692/ |
| _version_ | 1819058945610743808 |
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| author | Sónia Pérez-Rentero Ramón Eritja Alejandra V. Garibotti |
| author_facet | Sónia Pérez-Rentero Ramón Eritja Alejandra V. Garibotti |
| author_sort | Sónia Pérez-Rentero |
| collection | DOAJ |
| description | An efficient route for the synthesis of the phosphoramidite derivative of 5-methylcytosine bearing a tert-butylsulfanyl group protected thiol is described. This building block is used for the preparation of oligonucleotides carrying a thiol group at the nucleobase at the internal position of a DNA sequence. The resulting thiolated oligonucleotides are useful intermediates to generate oligonucleotide conjugates carrying molecules of interest at internal positions of a DNA sequence. |
| first_indexed | 2024-12-21T14:03:16Z |
| format | Article |
| id | doaj.art-861b0e8628e24f8e8b23b762f015c901 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T14:03:16Z |
| publishDate | 2010-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-861b0e8628e24f8e8b23b762f015c9012022-12-21T19:01:19ZengMDPI AGMolecules1420-30492010-08-011585692570710.3390/molecules15085692Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine MoietiesSónia Pérez-RenteroRamón EritjaAlejandra V. GaribottiAn efficient route for the synthesis of the phosphoramidite derivative of 5-methylcytosine bearing a tert-butylsulfanyl group protected thiol is described. This building block is used for the preparation of oligonucleotides carrying a thiol group at the nucleobase at the internal position of a DNA sequence. The resulting thiolated oligonucleotides are useful intermediates to generate oligonucleotide conjugates carrying molecules of interest at internal positions of a DNA sequence.http://www.mdpi.com/1420-3049/15/8/5692/solid-phase synthesisoligonucleotidesDNAthiololigonucleotide conjugates |
| spellingShingle | Sónia Pérez-Rentero Ramón Eritja Alejandra V. Garibotti Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties Molecules solid-phase synthesis oligonucleotides DNA thiol oligonucleotide conjugates |
| title | Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties |
| title_full | Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties |
| title_fullStr | Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties |
| title_full_unstemmed | Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties |
| title_short | Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties |
| title_sort | solid phase synthesis of oligodeoxynucleotides containing n4 2 t butyldisulfanyl ethyl 5 methylcytosine moieties |
| topic | solid-phase synthesis oligonucleotides DNA thiol oligonucleotide conjugates |
| url | http://www.mdpi.com/1420-3049/15/8/5692/ |
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