Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula
Background: Cronobacter species is the most significant foodborne pathogens in infant milk formula (IMF). These pathogens have been incriminated in sever form of neonatal meningitis, sepsis and necrotizing enterocolitis with high mortality rate. Aim: The current study was performed to demonstrate...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tripoli University
2023-02-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=138198 |
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| author | Aboubaker M. Garbaj Samira A. Farag Jihan A. Sherif Aml F. Lawila Hanan L. Eshamah Salah M. Azwai Fatim T. Gammoudi Hesham T. Naas Allaaeddin A. El-Salabi Ibrahim M. Eldaghayes |
| author_facet | Aboubaker M. Garbaj Samira A. Farag Jihan A. Sherif Aml F. Lawila Hanan L. Eshamah Salah M. Azwai Fatim T. Gammoudi Hesham T. Naas Allaaeddin A. El-Salabi Ibrahim M. Eldaghayes |
| author_sort | Aboubaker M. Garbaj |
| collection | DOAJ |
| description | Background: Cronobacter species is the most significant foodborne pathogens in infant milk formula (IMF). These pathogens have been incriminated in sever form of neonatal meningitis, sepsis and necrotizing enterocolitis with high mortality rate.
Aim: The current study was performed to demonstrate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF).
Methods: The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48, 52, 56, 60, 64 and 66°C. The D- and z-values were determined by using linear regression analysis.
Results: The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5 and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C ranged from 7.29 ˗ 23.47, 2.77˗15.50, 0.62˗1.04, 0.62˗1.02, 0.62˗1.00, 0.62˗1.00 min, respectively, while the z˗ values extended from 2.50˗4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) ranged from 7.60 ˗ 22.32, 1.42 ˗ 8.45, 0.62 ˗ 1.08, 0.62 ˗ 0.78, 0.62 ˗ 0.78, 0.62 ˗ 0.79 min. at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33 ˗ 4.89°C.
Conclusion: This study may contribute to improve the understanding of behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperature and may participate in effective control of this pathogens in infant's foods production. [Open Vet J 2023; 13(1.000): 108-113] |
| first_indexed | 2024-04-10T19:38:52Z |
| format | Article |
| id | doaj.art-861c95530b8e428aa97747f96ed2c510 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 |
| language | English |
| last_indexed | 2024-04-10T19:38:52Z |
| publishDate | 2023-02-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-861c95530b8e428aa97747f96ed2c5102023-01-29T17:41:57ZengTripoli UniversityOpen Veterinary Journal2226-44852023-02-0113110811310.5455/OVJ.2023.v13.i1.11138198Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formulaAboubaker M. Garbaj0Samira A. FaragJihan A. SherifAml F. LawilaHanan L. EshamahSalah M. AzwaiFatim T. GammoudiHesham T. NaasAllaaeddin A. El-SalabiIbrahim M. EldaghayesFaculty of Veterinary MedicineBackground: Cronobacter species is the most significant foodborne pathogens in infant milk formula (IMF). These pathogens have been incriminated in sever form of neonatal meningitis, sepsis and necrotizing enterocolitis with high mortality rate. Aim: The current study was performed to demonstrate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF). Methods: The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48, 52, 56, 60, 64 and 66°C. The D- and z-values were determined by using linear regression analysis. Results: The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5 and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C ranged from 7.29 ˗ 23.47, 2.77˗15.50, 0.62˗1.04, 0.62˗1.02, 0.62˗1.00, 0.62˗1.00 min, respectively, while the z˗ values extended from 2.50˗4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) ranged from 7.60 ˗ 22.32, 1.42 ˗ 8.45, 0.62 ˗ 1.08, 0.62 ˗ 0.78, 0.62 ˗ 0.78, 0.62 ˗ 0.79 min. at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33 ˗ 4.89°C. Conclusion: This study may contribute to improve the understanding of behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperature and may participate in effective control of this pathogens in infant's foods production. [Open Vet J 2023; 13(1.000): 108-113]http://www.ejmanager.com/fulltextpdf.php?mno=138198cronobacter sakazakiicronobacter pulveristhermo-toleranced˗ valuez˗ valueinfant milk formula |
| spellingShingle | Aboubaker M. Garbaj Samira A. Farag Jihan A. Sherif Aml F. Lawila Hanan L. Eshamah Salah M. Azwai Fatim T. Gammoudi Hesham T. Naas Allaaeddin A. El-Salabi Ibrahim M. Eldaghayes Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula Open Veterinary Journal cronobacter sakazakii cronobacter pulveris thermo-tolerance d˗ value z˗ value infant milk formula |
| title | Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula |
| title_full | Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula |
| title_fullStr | Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula |
| title_full_unstemmed | Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula |
| title_short | Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula |
| title_sort | thermal tolerance of cronobacter sakazakii and cronobacter pulveris in reconstituted infant milk formula |
| topic | cronobacter sakazakii cronobacter pulveris thermo-tolerance d˗ value z˗ value infant milk formula |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=138198 |
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