Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts
The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDF...
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MDPI AG
2013-02-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/2/2200 |
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| author | Suzana Makpol Tengku Ahbrizal Farizal Tengku Ahmad Yasmin Anum Mohd Yusof Nor Fadilah Rajab Khairuddin Abdul Rahim Zakiah Jubri |
| author_facet | Suzana Makpol Tengku Ahbrizal Farizal Tengku Ahmad Yasmin Anum Mohd Yusof Nor Fadilah Rajab Khairuddin Abdul Rahim Zakiah Jubri |
| author_sort | Suzana Makpol |
| collection | DOAJ |
| description | The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs treated with Gelam honey alone showed up-regulation of all genes studied. Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with gamma-irradiation and increased when cells were treated with Gelam honey (p < 0.05). Furthermore, of the three different stages of study treatment, pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes expression and increased the activity of SOD and CAT. As a conclusion, Gelam honey modulates the expression of antioxidant enzymes at gene and protein levels in irradiated HDFs indicating its potential as a radioprotectant agent. |
| first_indexed | 2024-12-10T09:44:05Z |
| format | Article |
| id | doaj.art-861ccca6c19e42c79760fad421e27bfc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T09:44:05Z |
| publishDate | 2013-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-861ccca6c19e42c79760fad421e27bfc2022-12-22T01:53:54ZengMDPI AGMolecules1420-30492013-02-011822200221110.3390/molecules18022200Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid FibroblastsSuzana MakpolTengku Ahbrizal Farizal Tengku AhmadYasmin Anum Mohd YusofNor Fadilah RajabKhairuddin Abdul RahimZakiah JubriThe present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs treated with Gelam honey alone showed up-regulation of all genes studied. Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with gamma-irradiation and increased when cells were treated with Gelam honey (p < 0.05). Furthermore, of the three different stages of study treatment, pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes expression and increased the activity of SOD and CAT. As a conclusion, Gelam honey modulates the expression of antioxidant enzymes at gene and protein levels in irradiated HDFs indicating its potential as a radioprotectant agent.http://www.mdpi.com/1420-3049/18/2/2200Gelam honeygamma-irradiation damageantioxidant enzymeshuman diploid fibroblasts |
| spellingShingle | Suzana Makpol Tengku Ahbrizal Farizal Tengku Ahmad Yasmin Anum Mohd Yusof Nor Fadilah Rajab Khairuddin Abdul Rahim Zakiah Jubri Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts Molecules Gelam honey gamma-irradiation damage antioxidant enzymes human diploid fibroblasts |
| title | Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts |
| title_full | Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts |
| title_fullStr | Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts |
| title_full_unstemmed | Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts |
| title_short | Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts |
| title_sort | gelam honey protects against gamma irradiation damage to antioxidant enzymes in human diploid fibroblasts |
| topic | Gelam honey gamma-irradiation damage antioxidant enzymes human diploid fibroblasts |
| url | http://www.mdpi.com/1420-3049/18/2/2200 |
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