Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’
Rose is the most economically important ornamental plant. However, cold stress seriously affects the survival and regrowth of garden roses in northern regions. Cold acclimation was studied using two genotypes (<i>Rosa wichurana</i> and <i>R. hybrida</i> ‘Yesterday’) selected...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/10/379 |
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| author | Lin Ouyang Leen Leus Ellen De Keyser Marie-Christine Van Labeke |
| author_facet | Lin Ouyang Leen Leus Ellen De Keyser Marie-Christine Van Labeke |
| author_sort | Lin Ouyang |
| collection | DOAJ |
| description | Rose is the most economically important ornamental plant. However, cold stress seriously affects the survival and regrowth of garden roses in northern regions. Cold acclimation was studied using two genotypes (<i>Rosa wichurana</i> and <i>R. hybrida</i> ‘Yesterday’) selected from a rose breeding program. During the winter season (November to April), the cold hardiness of stems, soluble sugar content, and expression of dehydrins and the related key genes in the soluble sugar metabolism were analyzed. ‘Yesterday’ is more cold-hardy and acclimated faster, reaching its maximum cold hardiness in December. <i>R. wichurana</i> is relatively less cold-hardy, only reaching its maximum cold hardiness in January after prolonged exposure to freezing temperatures. Dehydrin transcripts accumulated significantly during November–January in both genotypes. Soluble sugars are highly involved in cold acclimation, with sucrose and oligosaccharides significantly correlated with cold hardiness. Sucrose occupied the highest proportion of total soluble sugars in both genotypes. During November–January, downregulation of <i>RhSUS</i> was found in both genotypes, while upregulation of <i>RhSPS</i> was observed in ‘Yesterday’ and upregulation of <i>RhINV2</i> was found in <i>R. wichurana</i>. Oligosaccharides accumulated from November to February and decreased to a significantly low level in April. <i>RhRS6</i> had a significant upregulation in December in <i>R. wichurana</i>. This study provides insight into the cold acclimation mechanism of roses by combining transcription patterns with metabolite quantification. |
| first_indexed | 2024-03-10T06:31:51Z |
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| id | doaj.art-861cef6372f64dd6ad57dc70af1e9cfd |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T06:31:51Z |
| publishDate | 2021-10-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-861cef6372f64dd6ad57dc70af1e9cfd2023-11-22T18:27:24ZengMDPI AGHorticulturae2311-75242021-10-0171037910.3390/horticulturae7100379Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’Lin Ouyang0Leen Leus1Ellen De Keyser2Marie-Christine Van Labeke3Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 99 Hupan West Road, Tianfu New Area, Chengdu 610000, ChinaPlant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Caritasstraat 39, 9090 Melle, BelgiumPlant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Caritasstraat 39, 9090 Melle, BelgiumDepartment of Plants and Crops, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumRose is the most economically important ornamental plant. However, cold stress seriously affects the survival and regrowth of garden roses in northern regions. Cold acclimation was studied using two genotypes (<i>Rosa wichurana</i> and <i>R. hybrida</i> ‘Yesterday’) selected from a rose breeding program. During the winter season (November to April), the cold hardiness of stems, soluble sugar content, and expression of dehydrins and the related key genes in the soluble sugar metabolism were analyzed. ‘Yesterday’ is more cold-hardy and acclimated faster, reaching its maximum cold hardiness in December. <i>R. wichurana</i> is relatively less cold-hardy, only reaching its maximum cold hardiness in January after prolonged exposure to freezing temperatures. Dehydrin transcripts accumulated significantly during November–January in both genotypes. Soluble sugars are highly involved in cold acclimation, with sucrose and oligosaccharides significantly correlated with cold hardiness. Sucrose occupied the highest proportion of total soluble sugars in both genotypes. During November–January, downregulation of <i>RhSUS</i> was found in both genotypes, while upregulation of <i>RhSPS</i> was observed in ‘Yesterday’ and upregulation of <i>RhINV2</i> was found in <i>R. wichurana</i>. Oligosaccharides accumulated from November to February and decreased to a significantly low level in April. <i>RhRS6</i> had a significant upregulation in December in <i>R. wichurana</i>. This study provides insight into the cold acclimation mechanism of roses by combining transcription patterns with metabolite quantification.https://www.mdpi.com/2311-7524/7/10/379cold hardinessLT<sub>50</sub>sucroseoligosaccharidessoluble sugar metabolismgene expression |
| spellingShingle | Lin Ouyang Leen Leus Ellen De Keyser Marie-Christine Van Labeke Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’ Horticulturae cold hardiness LT<sub>50</sub> sucrose oligosaccharides soluble sugar metabolism gene expression |
| title | Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’ |
| title_full | Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’ |
| title_fullStr | Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’ |
| title_full_unstemmed | Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’ |
| title_short | Dehydrins and Soluble Sugars Involved in Cold Acclimation of <i>Rosa wichurana</i> and Rose Cultivar ‘Yesterday’ |
| title_sort | dehydrins and soluble sugars involved in cold acclimation of i rosa wichurana i and rose cultivar yesterday |
| topic | cold hardiness LT<sub>50</sub> sucrose oligosaccharides soluble sugar metabolism gene expression |
| url | https://www.mdpi.com/2311-7524/7/10/379 |
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