| Summary: | <p>Abstract</p> <p>Background</p> <p>In this report the phytochemical profile of <it>Nitraria. Retusa (N. Retusa</it>) leaf extracts were identified and their ability to induce apoptosis in human chronic myelogenous erythroleukaemia (K562) was evaluated.</p> <p>Methods</p> <p>Apoptosis of the human chronic myelogenous erythroleukaemia (K562) was evidenced by investigating DNA fragmentation, PARP cleavage and caspases 3 and 8 inducing activities, in the presence of <it>N. retusa </it>extracts.</p> <p>Results</p> <p>Our study revealed that the tested extracts from <it>N. Retusa </it>contain many useful bioactive compounds. They induced in a time-dependent manner the apoptosis the tested cancerous our cell line. This result was confirmed by ladder DNA fragmentation profile and PARP cleavage, as well as a release in caspase-3 and caspase-8 level.</p> <p>Conclusion</p> <p>Our results indicate that the tested compounds have a significant antiproliferative effect which may be due to their involvement in the induction of the extrinsic apoptosic pathway.</p>
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