Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
Classical swine fever virus (CSFV) remains a highly important pathogen, causing major losses in the swine industry. Persistent infection is highly relevant for CSFV maintenance in the field; however, this form of infection is not fully understood. An increase in the granulocyte population has been d...
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MDPI AG
2019-09-01
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Online Access: | https://www.mdpi.com/1999-4915/11/9/822 |
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author | Jose Alejandro Bohorquez Sara Muñoz-González Marta Pérez-Simó Concepción Revilla Javier Domínguez Llilianne Ganges |
author_facet | Jose Alejandro Bohorquez Sara Muñoz-González Marta Pérez-Simó Concepción Revilla Javier Domínguez Llilianne Ganges |
author_sort | Jose Alejandro Bohorquez |
collection | DOAJ |
description | Classical swine fever virus (CSFV) remains a highly important pathogen, causing major losses in the swine industry. Persistent infection is highly relevant for CSFV maintenance in the field; however, this form of infection is not fully understood. An increase in the granulocyte population has been detected in CSFV persistently infected animals. The aim of this work was to evaluate the possible immunosuppressive role of these cells in CSFV persistent infection. The phenotype of peripheral blood and bone marrow cells from persistently infected and naïve animals was evaluated by flow cytometry, and the capacity of specific cell subsets to reduce the interferon gamma (IFN-γ) response against unspecific and specific antigen was determined using co-culture assays. The frequency of granulocytic cells was increased in cells from CSFV persistently infected pigs and they showed a phenotype similar to immunosuppressive cell populations found in persistent infection in humans. These cells from persistently infected animals were able to reduce the IFN-γ response against unspecific and specific antigen. Our results suggest that immature immunosuppressive cell populations play a role in CSFV persistent infection in swine. The information obtained by studying the role of myeloid derived suppressor cells (MDSC) during CSFV persistent infection may extrapolate to other viral persistent infections in mammals. |
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id | doaj.art-861dc7b9fb014611b6acb4ba789a9918 |
institution | Directory Open Access Journal |
issn | 1999-4915 |
language | English |
last_indexed | 2024-04-12T05:38:54Z |
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publisher | MDPI AG |
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series | Viruses |
spelling | doaj.art-861dc7b9fb014611b6acb4ba789a99182022-12-22T03:45:44ZengMDPI AGViruses1999-49152019-09-0111982210.3390/v11090822v11090822Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the HostJose Alejandro Bohorquez0Sara Muñoz-González1Marta Pérez-Simó2Concepción Revilla3Javier Domínguez4Llilianne Ganges5OIE Reference Laboratory for classical swine fever, IRTA-CReSA, Campus Universitat de Barcelona, 08193 Bellaterra, SpainOIE Reference Laboratory for classical swine fever, IRTA-CReSA, Campus Universitat de Barcelona, 08193 Bellaterra, SpainOIE Reference Laboratory for classical swine fever, IRTA-CReSA, Campus Universitat de Barcelona, 08193 Bellaterra, SpainDpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, SpainDpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, SpainOIE Reference Laboratory for classical swine fever, IRTA-CReSA, Campus Universitat de Barcelona, 08193 Bellaterra, SpainClassical swine fever virus (CSFV) remains a highly important pathogen, causing major losses in the swine industry. Persistent infection is highly relevant for CSFV maintenance in the field; however, this form of infection is not fully understood. An increase in the granulocyte population has been detected in CSFV persistently infected animals. The aim of this work was to evaluate the possible immunosuppressive role of these cells in CSFV persistent infection. The phenotype of peripheral blood and bone marrow cells from persistently infected and naïve animals was evaluated by flow cytometry, and the capacity of specific cell subsets to reduce the interferon gamma (IFN-γ) response against unspecific and specific antigen was determined using co-culture assays. The frequency of granulocytic cells was increased in cells from CSFV persistently infected pigs and they showed a phenotype similar to immunosuppressive cell populations found in persistent infection in humans. These cells from persistently infected animals were able to reduce the IFN-γ response against unspecific and specific antigen. Our results suggest that immature immunosuppressive cell populations play a role in CSFV persistent infection in swine. The information obtained by studying the role of myeloid derived suppressor cells (MDSC) during CSFV persistent infection may extrapolate to other viral persistent infections in mammals.https://www.mdpi.com/1999-4915/11/9/822CSFVviral persistenceimmunosuppressionperipheral bloodbone marrow cells6D10 cellsMDSCinterferon gamma |
spellingShingle | Jose Alejandro Bohorquez Sara Muñoz-González Marta Pérez-Simó Concepción Revilla Javier Domínguez Llilianne Ganges Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host Viruses CSFV viral persistence immunosuppression peripheral blood bone marrow cells 6D10 cells MDSC interferon gamma |
title | Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host |
title_full | Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host |
title_fullStr | Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host |
title_full_unstemmed | Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host |
title_short | Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host |
title_sort | identification of an immunosuppressive cell population during classical swine fever virus infection and its role in viral persistence in the host |
topic | CSFV viral persistence immunosuppression peripheral blood bone marrow cells 6D10 cells MDSC interferon gamma |
url | https://www.mdpi.com/1999-4915/11/9/822 |
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