Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro
β-Glucan is linear polysaccharides containing d-glucose monomers connected by β-glycosidic linkages. Their structural variances are the result of several sources. This research project was designed to assess the anticancer activity by using β-glucan. The in vitro experiment employed breast cancer c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mustansiriyah University
2024-03-01
|
| Series: | Al-Mustansiriyah Journal of Science |
| Subjects: | |
| Online Access: | https://mjs.uomustansiriyah.edu.iq/index.php/MJS/article/view/1387 |
| _version_ | 1826994334992433152 |
|---|---|
| author | Hiba Muhammed Al-Khuzaay Yasir Hussein Al-Juraisy Ali Hussein Alwan Ehab Tousson |
| author_facet | Hiba Muhammed Al-Khuzaay Yasir Hussein Al-Juraisy Ali Hussein Alwan Ehab Tousson |
| author_sort | Hiba Muhammed Al-Khuzaay |
| collection | DOAJ |
| description |
β-Glucan is linear polysaccharides containing d-glucose monomers connected by β-glycosidic linkages. Their structural variances are the result of several sources. This research project was designed to assess the anticancer activity by using β-glucan. The in vitro experiment employed breast cancer cell lines from Michigan Cancer Foundation-7 (MCF-7) and Ahmed, Murtudha, Jabriyah, 2013 (AMJ13). After 24, 48, and 72 hours in micro titration plate under completely sterile condition. Different concentrations of β-glucan (31.25, 62.5, 125, 250, 500, and 1000 μg/mL) were applied to the cancer cell lines. The MTT assay was used to check whether the cells had been inhibited. Cell viability in MCF7 and AMJ13 cells was significantly reduced by β-glucan. β-Glucan showed concentration and time-dependent growth inhibitory effects. The higher concentrations of β-glucan significantly (P<0.05) decrease the growth rate of cells, indicating that the higher concentrations were more effective at inhibiting growth.
|
| first_indexed | 2024-04-24T15:41:51Z |
| format | Article |
| id | doaj.art-861dff86798444099a67fedcf539faad |
| institution | Directory Open Access Journal |
| issn | 1814-635X 2521-3520 |
| language | English |
| last_indexed | 2025-02-18T09:17:09Z |
| publishDate | 2024-03-01 |
| publisher | Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Science |
| spelling | doaj.art-861dff86798444099a67fedcf539faad2024-11-02T22:38:17ZengMustansiriyah UniversityAl-Mustansiriyah Journal of Science1814-635X2521-35202024-03-0135110.23851/mjs.v35i1.1387Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitroHiba Muhammed Al-Khuzaay0https://orcid.org/0000-0001-8989-0254Yasir Hussein Al-Juraisy1Ali Hussein Alwan2Ehab Tousson3Department of Biology, College of Science, Mustansiriyah University, 10052 Baghdad, IRAQ. Department of Biology, College of Science, Mustansiriyah University, 10052 Baghdad, IRAQ.Iraqi Center for Cancer and Medical Genetics Research, Mustansiriyah University, 10001 Baghdad, IRAQ.Zoology department, Faculty of Science, Tanta University, EGYPT. β-Glucan is linear polysaccharides containing d-glucose monomers connected by β-glycosidic linkages. Their structural variances are the result of several sources. This research project was designed to assess the anticancer activity by using β-glucan. The in vitro experiment employed breast cancer cell lines from Michigan Cancer Foundation-7 (MCF-7) and Ahmed, Murtudha, Jabriyah, 2013 (AMJ13). After 24, 48, and 72 hours in micro titration plate under completely sterile condition. Different concentrations of β-glucan (31.25, 62.5, 125, 250, 500, and 1000 μg/mL) were applied to the cancer cell lines. The MTT assay was used to check whether the cells had been inhibited. Cell viability in MCF7 and AMJ13 cells was significantly reduced by β-glucan. β-Glucan showed concentration and time-dependent growth inhibitory effects. The higher concentrations of β-glucan significantly (P<0.05) decrease the growth rate of cells, indicating that the higher concentrations were more effective at inhibiting growth. https://mjs.uomustansiriyah.edu.iq/index.php/MJS/article/view/1387β-glucanMTTAnticancerAMJ13MCF-7 |
| spellingShingle | Hiba Muhammed Al-Khuzaay Yasir Hussein Al-Juraisy Ali Hussein Alwan Ehab Tousson Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro Al-Mustansiriyah Journal of Science β-glucan MTT Anticancer AMJ13 MCF-7 |
| title | Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro |
| title_full | Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro |
| title_fullStr | Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro |
| title_full_unstemmed | Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro |
| title_short | Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro |
| title_sort | evaluation of effect of β glucan on cancer cell lines in vitro |
| topic | β-glucan MTT Anticancer AMJ13 MCF-7 |
| url | https://mjs.uomustansiriyah.edu.iq/index.php/MJS/article/view/1387 |
| work_keys_str_mv | AT hibamuhammedalkhuzaay evaluationofeffectofbglucanoncancercelllinesinvitro AT yasirhusseinaljuraisy evaluationofeffectofbglucanoncancercelllinesinvitro AT alihusseinalwan evaluationofeffectofbglucanoncancercelllinesinvitro AT ehabtousson evaluationofeffectofbglucanoncancercelllinesinvitro |