Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process
Anaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g., sulfamethox...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.537783/full |
| _version_ | 1811192923418525696 |
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| author | Valentina Mazzurco Miritana Giulia Massini Giulia Massini Andrea Visca Paola Grenni Luisa Patrolecco Francesca Spataro Jasmin Rauseo Gian Luigi Garbini Antonella Signorini Silvia Rosa Anna Barra Caracciolo |
| author_facet | Valentina Mazzurco Miritana Giulia Massini Giulia Massini Andrea Visca Paola Grenni Luisa Patrolecco Francesca Spataro Jasmin Rauseo Gian Luigi Garbini Antonella Signorini Silvia Rosa Anna Barra Caracciolo |
| author_sort | Valentina Mazzurco Miritana |
| collection | DOAJ |
| description | Anaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g., sulfamethoxazole) in animal husbandry practices is showing increasing negative impacts resulting from the release of still metabolically active molecules into agroecosystems. In the present study, cattle manure collected from an AD plant-feeding tank was used as feedstock for AD experiments in which some batches were spiked with 5 mg L–1 of sulfamethoxazole (SMX). Adding the antibiotic affected the microbial community dynamic; in particular, the efficiency of the acidogenic and acetogenic phases of the process corresponded to higher CH4 and H2 production than in the control. SMX was also degraded, and at the end of the experiment (69 days), just 20% of its initial concentration was found. The relative abundance (ARG/16S) of resistance genes sul1, sul2, and the proxy intI1 initially found in the ingestate decreased during the AD in both the spiked and control batches, suggesting that this process lowers the likelihood of antibiotic resistance genes spreading. |
| first_indexed | 2024-04-11T23:58:38Z |
| format | Article |
| id | doaj.art-861f175cc4444550805d6a398a18cbed |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T23:58:38Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-861f175cc4444550805d6a398a18cbed2022-12-22T03:56:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-09-011110.3389/fmicb.2020.537783537783Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion ProcessValentina Mazzurco Miritana0Giulia Massini1Giulia Massini2Andrea Visca3Paola Grenni4Luisa Patrolecco5Francesca Spataro6Jasmin Rauseo7Gian Luigi Garbini8Antonella Signorini9Silvia Rosa10Anna Barra Caracciolo11Water Research Institute, National Research Council, Montelibretti, ItalyWater Research Institute, National Research Council, Montelibretti, ItalyDepartment of Energy Technologies, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, ItalyWater Research Institute, National Research Council, Montelibretti, ItalyWater Research Institute, National Research Council, Montelibretti, ItalyInstitute of Polar Sciences, National Research Council, Montelibretti, ItalyInstitute of Polar Sciences, National Research Council, Montelibretti, ItalyInstitute of Polar Sciences, National Research Council, Montelibretti, ItalyWater Research Institute, National Research Council, Montelibretti, ItalyDepartment of Energy Technologies, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, ItalyDepartment of Energy Technologies, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, ItalyWater Research Institute, National Research Council, Montelibretti, ItalyAnaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g., sulfamethoxazole) in animal husbandry practices is showing increasing negative impacts resulting from the release of still metabolically active molecules into agroecosystems. In the present study, cattle manure collected from an AD plant-feeding tank was used as feedstock for AD experiments in which some batches were spiked with 5 mg L–1 of sulfamethoxazole (SMX). Adding the antibiotic affected the microbial community dynamic; in particular, the efficiency of the acidogenic and acetogenic phases of the process corresponded to higher CH4 and H2 production than in the control. SMX was also degraded, and at the end of the experiment (69 days), just 20% of its initial concentration was found. The relative abundance (ARG/16S) of resistance genes sul1, sul2, and the proxy intI1 initially found in the ingestate decreased during the AD in both the spiked and control batches, suggesting that this process lowers the likelihood of antibiotic resistance genes spreading.https://www.frontiersin.org/article/10.3389/fmicb.2020.537783/fullcattle manuresulfamethoxazolebiogasanaerobic digestionmicrobial communityantibiotic degradation |
| spellingShingle | Valentina Mazzurco Miritana Giulia Massini Giulia Massini Andrea Visca Paola Grenni Luisa Patrolecco Francesca Spataro Jasmin Rauseo Gian Luigi Garbini Antonella Signorini Silvia Rosa Anna Barra Caracciolo Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process Frontiers in Microbiology cattle manure sulfamethoxazole biogas anaerobic digestion microbial community antibiotic degradation |
| title | Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process |
| title_full | Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process |
| title_fullStr | Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process |
| title_full_unstemmed | Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process |
| title_short | Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process |
| title_sort | effects of sulfamethoxazole on the microbial community dynamics during the anaerobic digestion process |
| topic | cattle manure sulfamethoxazole biogas anaerobic digestion microbial community antibiotic degradation |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.537783/full |
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