Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography
Aim: The aim of this study was to comparatively assess the gelatinolytic activity of matrix metalloproteinases(MMPs) and Cysteine Cathepsins (CCs) in the adhesive interface using etch and rinse adhesive at different time intervals using zymographic technique. Methodology: Twenty freshly extracted no...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Indian Journal of Dental Research |
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| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2016;volume=27;issue=6;spage=652;epage=656;aulast=Mahalaxmi |
| _version_ | 1818291374714257408 |
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| author | Sekar Mahalaxmi Manavalan Madhana Madhubala Mahendran Jayaraman Shanmugasundaram Sathyakumar |
| author_facet | Sekar Mahalaxmi Manavalan Madhana Madhubala Mahendran Jayaraman Shanmugasundaram Sathyakumar |
| author_sort | Sekar Mahalaxmi |
| collection | DOAJ |
| description | Aim: The aim of this study was to comparatively assess the gelatinolytic activity of matrix metalloproteinases(MMPs) and Cysteine Cathepsins (CCs) in the adhesive interface using etch and rinse adhesive at different time intervals using zymographic technique.
Methodology: Twenty freshly extracted non-carious human third molars were used in this study. Occlusal surfaces were ground flat and 1mm thick horizontal dentin slabs were obtained from each tooth using a diamond disc. The dentin surface was polished with 600-grit silicon-carbide paper. Five out of 20 samples were directly pulverized. In the remaining fifteen samples, the dentin was etched and adhesive was applied and light cured according to the manufacturer's instructions. A 1mm thick flowable composite was build up and light cured. Bonded specimens were cut vertically into 3 to 4 dentin slabs by means of diamond disc to expose the adhesive/dentin interfaces. These were then ground down to 500 µm thick resin-dentin interface using a hard tissue microtome. These sections were then pulverised into powder. Following this, every five samples were subjected to zymographic analysis after 1 day, 7 days and 21 days.
Results: Zymograms showed clear, thicker bands on all three isoforms in the etched samples compared to control samples at 1st and 7th day intervals and became inactive at 21st day for all three isoforms. MMP 9 activity was relatively higher when compared to CCs and MMP 2.
Conclusion: Etch and rinse adhesive activated MMPs and CCs within the hybrid layer that remained active till 7th day and no gelatinolytic activity was found on 21st day and MMPs are more active compared to CCs and MMP-2. |
| first_indexed | 2024-12-13T02:43:03Z |
| format | Article |
| id | doaj.art-86209729d1b349ab9097cd9d271c4b09 |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-12-13T02:43:03Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-86209729d1b349ab9097cd9d271c4b092022-12-22T00:02:15ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032016-01-0127665265610.4103/0970-9290.199585Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymographySekar MahalaxmiManavalan Madhana MadhubalaMahendran JayaramanShanmugasundaram SathyakumarAim: The aim of this study was to comparatively assess the gelatinolytic activity of matrix metalloproteinases(MMPs) and Cysteine Cathepsins (CCs) in the adhesive interface using etch and rinse adhesive at different time intervals using zymographic technique. Methodology: Twenty freshly extracted non-carious human third molars were used in this study. Occlusal surfaces were ground flat and 1mm thick horizontal dentin slabs were obtained from each tooth using a diamond disc. The dentin surface was polished with 600-grit silicon-carbide paper. Five out of 20 samples were directly pulverized. In the remaining fifteen samples, the dentin was etched and adhesive was applied and light cured according to the manufacturer's instructions. A 1mm thick flowable composite was build up and light cured. Bonded specimens were cut vertically into 3 to 4 dentin slabs by means of diamond disc to expose the adhesive/dentin interfaces. These were then ground down to 500 µm thick resin-dentin interface using a hard tissue microtome. These sections were then pulverised into powder. Following this, every five samples were subjected to zymographic analysis after 1 day, 7 days and 21 days. Results: Zymograms showed clear, thicker bands on all three isoforms in the etched samples compared to control samples at 1st and 7th day intervals and became inactive at 21st day for all three isoforms. MMP 9 activity was relatively higher when compared to CCs and MMP 2. Conclusion: Etch and rinse adhesive activated MMPs and CCs within the hybrid layer that remained active till 7th day and no gelatinolytic activity was found on 21st day and MMPs are more active compared to CCs and MMP-2.http://www.ijdr.in/article.asp?issn=0970-9290;year=2016;volume=27;issue=6;spage=652;epage=656;aulast=MahalaxmiCysteine cathepsinetch-and-rinse adhesiveshybrid layermatrix metalloproteinasezymography |
| spellingShingle | Sekar Mahalaxmi Manavalan Madhana Madhubala Mahendran Jayaraman Shanmugasundaram Sathyakumar Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography Indian Journal of Dental Research Cysteine cathepsin etch-and-rinse adhesives hybrid layer matrix metalloproteinase zymography |
| title | Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography |
| title_full | Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography |
| title_fullStr | Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography |
| title_full_unstemmed | Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography |
| title_short | Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography |
| title_sort | evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography |
| topic | Cysteine cathepsin etch-and-rinse adhesives hybrid layer matrix metalloproteinase zymography |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2016;volume=27;issue=6;spage=652;epage=656;aulast=Mahalaxmi |
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