Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family
Antimicrobial susceptibility testing is necessary to carry out antimicrobial stewardship but a limited number of drugs belonging to each antimicrobial family has to be tested for technical limitations and economic resources. In this study, we have determined the minimal inhibitory concentration, usi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/5/638 |
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| author | Anna Vilaró Elena Novell Vicens Enrique-Tarancon Jordi Balielles Lourdes Migura-García Lorenzo Fraile |
| author_facet | Anna Vilaró Elena Novell Vicens Enrique-Tarancon Jordi Balielles Lourdes Migura-García Lorenzo Fraile |
| author_sort | Anna Vilaró |
| collection | DOAJ |
| description | Antimicrobial susceptibility testing is necessary to carry out antimicrobial stewardship but a limited number of drugs belonging to each antimicrobial family has to be tested for technical limitations and economic resources. In this study, we have determined the minimal inhibitory concentration, using microdilution following international standards (CLSI), for 490 <i>Actinobacillus pleuropneumoniae</i>, 285 <i>Pasteurella multocida</i>, 73 <i>Bordetella bronchiseptica</i>, 398 <i>Streptococcus suis</i> and 1571 <i>Escherichia coli</i> strains from clinical cases collected in Spain between 2018 and 2020. The antimicrobial susceptibility pattern was deciphered using a principal component analysis for each bacterium and a matrix correlation (high > 0.8, medium 0.5–0.8 and low < 0.5) was obtained for each pair of antimicrobials. No significant associations were observed between MIC patterns for different antimicrobial families, suggesting that co-selection mechanisms are not generally present in these porcine pathogens. However, a high correlation was observed between the fluroquinolones (marbofloxacin and enrofloxacin) for all mentioned pathogens and for ceftiofur and cefquinome for <i>E. coli</i> and <i>S. suis</i>. Moreover, a significant association was also observed for tetracyclines (doxycycline and oxytetracycline) and <i>B. bronchiseptica</i> and tildipirosin/tulathromycin for <i>P. multocida</i>. These results suggest that generally, a representative drug per antimicrobial class cannot be selected, however, for some drug–bug combinations, MIC values from one representative drug could be extrapolated to the whole antimicrobial family. |
| first_indexed | 2024-03-10T03:27:24Z |
| format | Article |
| id | doaj.art-8622352cdad440db94f0954d8eb5271f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:27:24Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-8622352cdad440db94f0954d8eb5271f2023-11-23T09:48:59ZengMDPI AGAntibiotics2079-63822022-05-0111563810.3390/antibiotics11050638Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial FamilyAnna Vilaró0Elena Novell1Vicens Enrique-Tarancon2Jordi Balielles3Lourdes Migura-García4Lorenzo Fraile5Grup de Sanejament Porcí, 25192 Lleida, SpainGrup de Sanejament Porcí, 25192 Lleida, SpainGrup de Sanejament Porcí, 25192 Lleida, SpainGrup de Sanejament Porcí, 25192 Lleida, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), 08193 Bellaterra, SpainDepartament de Ciència Animal, ETSEA, University of Lleida-Agrotecnio, 25198 Lleida, SpainAntimicrobial susceptibility testing is necessary to carry out antimicrobial stewardship but a limited number of drugs belonging to each antimicrobial family has to be tested for technical limitations and economic resources. In this study, we have determined the minimal inhibitory concentration, using microdilution following international standards (CLSI), for 490 <i>Actinobacillus pleuropneumoniae</i>, 285 <i>Pasteurella multocida</i>, 73 <i>Bordetella bronchiseptica</i>, 398 <i>Streptococcus suis</i> and 1571 <i>Escherichia coli</i> strains from clinical cases collected in Spain between 2018 and 2020. The antimicrobial susceptibility pattern was deciphered using a principal component analysis for each bacterium and a matrix correlation (high > 0.8, medium 0.5–0.8 and low < 0.5) was obtained for each pair of antimicrobials. No significant associations were observed between MIC patterns for different antimicrobial families, suggesting that co-selection mechanisms are not generally present in these porcine pathogens. However, a high correlation was observed between the fluroquinolones (marbofloxacin and enrofloxacin) for all mentioned pathogens and for ceftiofur and cefquinome for <i>E. coli</i> and <i>S. suis</i>. Moreover, a significant association was also observed for tetracyclines (doxycycline and oxytetracycline) and <i>B. bronchiseptica</i> and tildipirosin/tulathromycin for <i>P. multocida</i>. These results suggest that generally, a representative drug per antimicrobial class cannot be selected, however, for some drug–bug combinations, MIC values from one representative drug could be extrapolated to the whole antimicrobial family.https://www.mdpi.com/2079-6382/11/5/638antimicrobialsusceptibility testingMICporcine bacterial pathogen |
| spellingShingle | Anna Vilaró Elena Novell Vicens Enrique-Tarancon Jordi Balielles Lourdes Migura-García Lorenzo Fraile Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family Antibiotics antimicrobial susceptibility testing MIC porcine bacterial pathogen |
| title | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
| title_full | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
| title_fullStr | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
| title_full_unstemmed | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
| title_short | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
| title_sort | antimicrobial susceptibility testing of porcine bacterial pathogens investigating the prospect of testing a representative drug for each antimicrobial family |
| topic | antimicrobial susceptibility testing MIC porcine bacterial pathogen |
| url | https://www.mdpi.com/2079-6382/11/5/638 |
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