Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation
(1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones w...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/11/596 |
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| author | Lin-Lin Zhang Xiao-Hua Jiang Xin-Feng Xiao Wen-Xiu Zhang Yi-Qian Shi Zhi-Peng Wang Hai-Xiang Zhou |
| author_facet | Lin-Lin Zhang Xiao-Hua Jiang Xin-Feng Xiao Wen-Xiu Zhang Yi-Qian Shi Zhi-Peng Wang Hai-Xiang Zhou |
| author_sort | Lin-Lin Zhang |
| collection | DOAJ |
| description | (1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones with high stability are still rather rare but required. (2) Methods: A novel chitosanase named CsnY from marine bacterium <i>Renibacterium</i> sp. Y82 was expressed in <i>Escherichia coli</i>, following sequence analysis. Then, the characterizations of recombinant CsnY purified through Ni–NTA affinity chromatography were conducted, including effects of pH and temperature, effects of metal ions and chemicals, and final product analysis. (3) Results: The GH46 family chitosanase CsnY possessed promising thermostability at broad temperature range (0–50 °C), and with optimal activity at 40 °C and pH 6.0, especially showing relatively high activity (over 80% of its maximum activity) at low temperatures (20–30 °C), which demonstrated the cold-adapted property. Common metal ions or chemicals had no obvious effect on CsnY except Mn<sup>2+</sup> and Co<sup>2+</sup>. Finally, CsnY was determined to be an endo-type chitosanase generating chitodisaccharides and -trisaccharides as main products, whose total concentration reached 56.74 mM within 2 h against 2% (<i>w</i>/<i>v</i>) initial chitosan substrate. (4) Conclusions: The results suggest the cold-adapted CsnY with favorable stability has desirable potential for the industrial production of COS. |
| first_indexed | 2024-03-10T05:19:49Z |
| format | Article |
| id | doaj.art-8629524aad324803a6596d67ba7cb4b3 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T05:19:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-8629524aad324803a6596d67ba7cb4b32023-11-23T00:07:01ZengMDPI AGMarine Drugs1660-33972021-10-01191159610.3390/md19110596Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides PreparationLin-Lin Zhang0Xiao-Hua Jiang1Xin-Feng Xiao2Wen-Xiu Zhang3Yi-Qian Shi4Zhi-Peng Wang5Hai-Xiang Zhou6College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaCollege of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, ChinaCollege of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, ChinaCollege of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, ChinaMarine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China(1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones with high stability are still rather rare but required. (2) Methods: A novel chitosanase named CsnY from marine bacterium <i>Renibacterium</i> sp. Y82 was expressed in <i>Escherichia coli</i>, following sequence analysis. Then, the characterizations of recombinant CsnY purified through Ni–NTA affinity chromatography were conducted, including effects of pH and temperature, effects of metal ions and chemicals, and final product analysis. (3) Results: The GH46 family chitosanase CsnY possessed promising thermostability at broad temperature range (0–50 °C), and with optimal activity at 40 °C and pH 6.0, especially showing relatively high activity (over 80% of its maximum activity) at low temperatures (20–30 °C), which demonstrated the cold-adapted property. Common metal ions or chemicals had no obvious effect on CsnY except Mn<sup>2+</sup> and Co<sup>2+</sup>. Finally, CsnY was determined to be an endo-type chitosanase generating chitodisaccharides and -trisaccharides as main products, whose total concentration reached 56.74 mM within 2 h against 2% (<i>w</i>/<i>v</i>) initial chitosan substrate. (4) Conclusions: The results suggest the cold-adapted CsnY with favorable stability has desirable potential for the industrial production of COS.https://www.mdpi.com/1660-3397/19/11/596chitosanasecold adaptationchitooligosaccharide<i>Renibacterium</i> sp. |
| spellingShingle | Lin-Lin Zhang Xiao-Hua Jiang Xin-Feng Xiao Wen-Xiu Zhang Yi-Qian Shi Zhi-Peng Wang Hai-Xiang Zhou Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation Marine Drugs chitosanase cold adaptation chitooligosaccharide <i>Renibacterium</i> sp. |
| title | Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation |
| title_full | Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation |
| title_fullStr | Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation |
| title_full_unstemmed | Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation |
| title_short | Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation |
| title_sort | expression and characterization of a novel cold adapted chitosanase from marine i renibacterium i sp suitable for chitooligosaccharides preparation |
| topic | chitosanase cold adaptation chitooligosaccharide <i>Renibacterium</i> sp. |
| url | https://www.mdpi.com/1660-3397/19/11/596 |
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