Identification of Fibrinolytic Activity in Iranian Vipera Lebetina Venom
Background and purpose: Vipera lebetina lives in different areas in Iran, and its venom contains a variety of proteins with coagulant and anticoagulant activities. Fibrinolytic enzymes could have a therapeutic role in dissolution of blood clots, so, this study aimed at separating the venom component...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2020-09-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-13934-en.html |
| _version_ | 1797944907696963584 |
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| author | Zohreh Amoozgari Maryam Cheraghzadeh Mozhgan Noorbehbahani Nasrin Lamuchi Deli |
| author_facet | Zohreh Amoozgari Maryam Cheraghzadeh Mozhgan Noorbehbahani Nasrin Lamuchi Deli |
| author_sort | Zohreh Amoozgari |
| collection | DOAJ |
| description | Background and purpose: Vipera lebetina lives in different areas in Iran, and its venom contains a variety of proteins with coagulant and anticoagulant activities. Fibrinolytic enzymes could have a therapeutic role in dissolution of blood clots, so, this study aimed at separating the venom components of Iranian V. lebetina and detecting its anticoagulant activity.
Materials and methods: In this experimental study, crude venom components were isolated by gel filtration chromatography on sephadex G-100. We investigated the endopeptidase, arginine ester hydrolase, coagulant, anticoagulant, and fibrinolytic activities in crude venom and separated fractions.
Results: The crude venom was separated into five fractions (PI-PV). 200 mg of crude venom contained 187 mg protein and 11.75 mg protein was recovered from 187 mg protein used on the column. The venom showed coagulant activity at low concentrations and anticoagulant activity at high concentrations. Endopeptidase activity was detected in crude venom and all fractions except PV. Also, arginine ester hydrolase activity was seen in crude venom, PI, and PII. Fibrinolytic activity was found in crude venom and only in PIII.
Conclusion: According to this study, the venom of Iranian V. lebetina has strong proteolytic activities including fibrinolytic that dissolve blood clots by lysis fibrin directly in laboratory conditions. |
| first_indexed | 2024-04-10T20:47:03Z |
| format | Article |
| id | doaj.art-862b443c0eb34963ae293f853827fffd |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:47:03Z |
| publishDate | 2020-09-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-862b443c0eb34963ae293f853827fffd2023-01-24T06:31:35ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792020-09-01301881725Identification of Fibrinolytic Activity in Iranian Vipera Lebetina VenomZohreh Amoozgari0Maryam Cheraghzadeh1Mozhgan Noorbehbahani2Nasrin Lamuchi Deli3 Lecturer, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Assistant Professor, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Laboratory staff, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran MSc Student in Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Background and purpose: Vipera lebetina lives in different areas in Iran, and its venom contains a variety of proteins with coagulant and anticoagulant activities. Fibrinolytic enzymes could have a therapeutic role in dissolution of blood clots, so, this study aimed at separating the venom components of Iranian V. lebetina and detecting its anticoagulant activity. Materials and methods: In this experimental study, crude venom components were isolated by gel filtration chromatography on sephadex G-100. We investigated the endopeptidase, arginine ester hydrolase, coagulant, anticoagulant, and fibrinolytic activities in crude venom and separated fractions. Results: The crude venom was separated into five fractions (PI-PV). 200 mg of crude venom contained 187 mg protein and 11.75 mg protein was recovered from 187 mg protein used on the column. The venom showed coagulant activity at low concentrations and anticoagulant activity at high concentrations. Endopeptidase activity was detected in crude venom and all fractions except PV. Also, arginine ester hydrolase activity was seen in crude venom, PI, and PII. Fibrinolytic activity was found in crude venom and only in PIII. Conclusion: According to this study, the venom of Iranian V. lebetina has strong proteolytic activities including fibrinolytic that dissolve blood clots by lysis fibrin directly in laboratory conditions.http://jmums.mazums.ac.ir/article-1-13934-en.htmlvipera lebetina venomcoagulant activityanticoagulant activityendopeptidasearginine esterasefibrinolysis |
| spellingShingle | Zohreh Amoozgari Maryam Cheraghzadeh Mozhgan Noorbehbahani Nasrin Lamuchi Deli Identification of Fibrinolytic Activity in Iranian Vipera Lebetina Venom Journal of Mazandaran University of Medical Sciences vipera lebetina venom coagulant activity anticoagulant activity endopeptidase arginine esterase fibrinolysis |
| title | Identification of Fibrinolytic Activity in Iranian Vipera
Lebetina Venom |
| title_full | Identification of Fibrinolytic Activity in Iranian Vipera
Lebetina Venom |
| title_fullStr | Identification of Fibrinolytic Activity in Iranian Vipera
Lebetina Venom |
| title_full_unstemmed | Identification of Fibrinolytic Activity in Iranian Vipera
Lebetina Venom |
| title_short | Identification of Fibrinolytic Activity in Iranian Vipera
Lebetina Venom |
| title_sort | identification of fibrinolytic activity in iranian vipera lebetina venom |
| topic | vipera lebetina venom coagulant activity anticoagulant activity endopeptidase arginine esterase fibrinolysis |
| url | http://jmums.mazums.ac.ir/article-1-13934-en.html |
| work_keys_str_mv | AT zohrehamoozgari identificationoffibrinolyticactivityiniranianviperalebetinavenom AT maryamcheraghzadeh identificationoffibrinolyticactivityiniranianviperalebetinavenom AT mozhgannoorbehbahani identificationoffibrinolyticactivityiniranianviperalebetinavenom AT nasrinlamuchideli identificationoffibrinolyticactivityiniranianviperalebetinavenom |