A Simple Method for High-Throughput Extract Prefractionation for Biological Screening
The screening of crude natural product extracts can be problematic, especially in enzyme/protein-based assays, due to promiscuous inhibitors and interference compounds, while high salt and lipid content can significantly dilute compounds of interest. Described herein is a simple method for...
| Main Authors: | , , |
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| Format: | Article |
| Language: | deu |
| Published: |
Swiss Chemical Society
2007-06-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://chimia.ch/chimia/article/view/4334 |
| _version_ | 1811320075609702400 |
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| author | David R. Appleton Antony D. Buss Mark S. Butler |
| author_facet | David R. Appleton Antony D. Buss Mark S. Butler |
| author_sort | David R. Appleton |
| collection | DOAJ |
| description |
The screening of crude natural product extracts can be problematic, especially in enzyme/protein-based assays, due to promiscuous inhibitors and interference compounds, while high salt and lipid content can significantly dilute compounds of interest. Described herein is a simple method
for high-throughput fractionation of crude extracts that can help address some of these issues. Results obtained indicate a significant improvement in assay hit rates without a reduction of hit quality and show how prefractionation aids the discovery of compounds that would be undetected by
screening crude extracts alone.
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| first_indexed | 2024-04-13T12:53:07Z |
| format | Article |
| id | doaj.art-862d02f6c6b74ebcbc361cead15578a9 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-04-13T12:53:07Z |
| publishDate | 2007-06-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-862d02f6c6b74ebcbc361cead15578a92022-12-22T02:46:08ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242007-06-0161610.2533/chimia.2007.327A Simple Method for High-Throughput Extract Prefractionation for Biological ScreeningDavid R. AppletonAntony D. BussMark S. Butler0MerLion Pharmaceuticals Pte Ltd, 1 Science Park Road, The Capricorn #05-01, Singapore Science Park II, Singapore 117528, Tel: +65 6829 5600, Fax: +65 6829 5601, Email: mark@merlionpharma.com The screening of crude natural product extracts can be problematic, especially in enzyme/protein-based assays, due to promiscuous inhibitors and interference compounds, while high salt and lipid content can significantly dilute compounds of interest. Described herein is a simple method for high-throughput fractionation of crude extracts that can help address some of these issues. Results obtained indicate a significant improvement in assay hit rates without a reduction of hit quality and show how prefractionation aids the discovery of compounds that would be undetected by screening crude extracts alone. https://chimia.ch/chimia/article/view/4334AutomationHigh-throughput screeningNatural productPrefractionation |
| spellingShingle | David R. Appleton Antony D. Buss Mark S. Butler A Simple Method for High-Throughput Extract Prefractionation for Biological Screening CHIMIA Automation High-throughput screening Natural product Prefractionation |
| title | A Simple Method for High-Throughput Extract Prefractionation for Biological Screening |
| title_full | A Simple Method for High-Throughput Extract Prefractionation for Biological Screening |
| title_fullStr | A Simple Method for High-Throughput Extract Prefractionation for Biological Screening |
| title_full_unstemmed | A Simple Method for High-Throughput Extract Prefractionation for Biological Screening |
| title_short | A Simple Method for High-Throughput Extract Prefractionation for Biological Screening |
| title_sort | simple method for high throughput extract prefractionation for biological screening |
| topic | Automation High-throughput screening Natural product Prefractionation |
| url | https://chimia.ch/chimia/article/view/4334 |
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