Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish
Summary: Chromatin remodeling plays an important role in regulating gene transcription, in which chromatin remodeling complex is a crucial aspect. Brg1/Brm-associated factor 60c (BAF60c) subunit forms a bridge between chromatin remodeling complexes and transcription factors in mammals; hence, it has...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223022848 |
| _version_ | 1827781476674961408 |
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| author | Jie Sun Qiuxin Yan Zhihao Zhang Ting Xu Ye Gong Weijia Li Kangsen Mai Qinghui Ai |
| author_facet | Jie Sun Qiuxin Yan Zhihao Zhang Ting Xu Ye Gong Weijia Li Kangsen Mai Qinghui Ai |
| author_sort | Jie Sun |
| collection | DOAJ |
| description | Summary: Chromatin remodeling plays an important role in regulating gene transcription, in which chromatin remodeling complex is a crucial aspect. Brg1/Brm-associated factor 60c (BAF60c) subunit forms a bridge between chromatin remodeling complexes and transcription factors in mammals; hence, it has received extensive attention. However, the roles of BAF60c in fish remain largely unexplored. In this study, we identified BAF60c-interacting proteins by using HIS-pull-down and LC-MS/MS analysis in fish. Subsequently, the RNA-seq analysis was performed to identify the overall effects of BAF60c. Then, the function of BAF60c was verified through BAF60c knockdown and overexpression experiments. We demonstrated for the first time that BAF60c interacts with glucose-regulated protein 78 (GRP78) and regulates lipid metabolism, endoplasmic reticulum (ER) stress, and inflammation. Knockdown of BAF60c reduces fatty acid biosynthesis, ER stress, and inflammation. In conclusion, the results enriched BAF60c-interacting protein network and explored the function of BAF60c in lipid metabolism and inflammation in fish. |
| first_indexed | 2024-03-11T15:19:15Z |
| format | Article |
| id | doaj.art-862df2012ca04977b3d733cad9a936bd |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T15:19:15Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-862df2012ca04977b3d733cad9a936bd2023-10-29T04:20:07ZengElsevieriScience2589-00422023-11-012611108207Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fishJie Sun0Qiuxin Yan1Zhihao Zhang2Ting Xu3Ye Gong4Weijia Li5Kangsen Mai6Qinghui Ai7Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, People’s Republic of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People’s Republic of China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, People’s Republic of China; Corresponding authorSummary: Chromatin remodeling plays an important role in regulating gene transcription, in which chromatin remodeling complex is a crucial aspect. Brg1/Brm-associated factor 60c (BAF60c) subunit forms a bridge between chromatin remodeling complexes and transcription factors in mammals; hence, it has received extensive attention. However, the roles of BAF60c in fish remain largely unexplored. In this study, we identified BAF60c-interacting proteins by using HIS-pull-down and LC-MS/MS analysis in fish. Subsequently, the RNA-seq analysis was performed to identify the overall effects of BAF60c. Then, the function of BAF60c was verified through BAF60c knockdown and overexpression experiments. We demonstrated for the first time that BAF60c interacts with glucose-regulated protein 78 (GRP78) and regulates lipid metabolism, endoplasmic reticulum (ER) stress, and inflammation. Knockdown of BAF60c reduces fatty acid biosynthesis, ER stress, and inflammation. In conclusion, the results enriched BAF60c-interacting protein network and explored the function of BAF60c in lipid metabolism and inflammation in fish.http://www.sciencedirect.com/science/article/pii/S2589004223022848Biological sciencesZoologyPhysiologyMolecular biology |
| spellingShingle | Jie Sun Qiuxin Yan Zhihao Zhang Ting Xu Ye Gong Weijia Li Kangsen Mai Qinghui Ai Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish iScience Biological sciences Zoology Physiology Molecular biology |
| title | Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish |
| title_full | Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish |
| title_fullStr | Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish |
| title_full_unstemmed | Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish |
| title_short | Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish |
| title_sort | exploring the role of swi snf complex subunit baf60c in lipid metabolism and inflammation in fish |
| topic | Biological sciences Zoology Physiology Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223022848 |
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