Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique
An experiment was conducted to evaluate the effects of Folligon and Corulon (hormons) supplemention during IVM on porcine oocyte maturation and subsequent fertilization with intracytoplasmic sperm injection , clevage and embryo development. Cumulus-oocyte complexes were cultured 44 hours with 10 IU...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/1276 |
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| author | Iulian Roman Vasile Miclea Marius Zăhan Paul Tăpăloagă Andrea Hettig Ileana Miclea Alexandru Rusu Florin Ghiuru |
| author_facet | Iulian Roman Vasile Miclea Marius Zăhan Paul Tăpăloagă Andrea Hettig Ileana Miclea Alexandru Rusu Florin Ghiuru |
| author_sort | Iulian Roman |
| collection | DOAJ |
| description | An experiment was conducted to evaluate the effects of Folligon and Corulon (hormons) supplemention during IVM on porcine oocyte maturation and subsequent fertilization with intracytoplasmic sperm injection , clevage and embryo development. Cumulus-oocyte complexes were cultured 44 hours with 10 IU Folligon and 10 IU Chorulon (0-44 h), or 22 hours withe the same amount of hormons (0-22 h) followed by a 22 hours cultue period without hormons). Nuclear maturation was assesed after IVM culture by examining the morphology and the presence of the first polar body using the micromanipulation system. The oocytes were injected with a single sperm cell and cultured in NCSU-23 medium. The percentage of cleaved embryos were determined, also the embryo development.
The presence of Folligon and Chorulon during the first 22 hours of IVM gave beter results in the terms of polar body extrusion (63%) compared to the group exposed for 44 hours to the hormons (37%). After 6 days of culture , the number of advanced embyo development stage (more than 16 cells per embryo) was also significantly different in the 0-22 h group. |
| first_indexed | 2024-04-24T11:33:12Z |
| format | Article |
| id | doaj.art-8635642ba0824b619f3e9cc722bc4df5 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:33:12Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-8635642ba0824b619f3e9cc722bc4df52024-04-10T08:10:46ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-014313213211276Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecniqueIulian Roman0Vasile Miclea1Marius Zăhan2Paul Tăpăloagă3Andrea Hettig4Ileana Miclea5Alexandru Rusu6Florin Ghiuru7University of Agricultural Science and Veterinary Medicine of Cluj, 3-5 Calea Mănăştur Street, 400372, Cluj-Napoca, RoumaniaUniversity of Agricultural Science and Veterinary Medicine of Cluj, 3-5 Calea Mănăştur Street, 400372, Cluj-Napoca, RoumaniaUniversity of Agricultural Science and Veterinary Medicine of Cluj, 3-5 Calea Mănăştur Street, 400372, Cluj-Napoca, RoumaniaUniversity of Agricultural Science and Veterinary Medicine Bucharest, 59 Mărăşti Blvd. 011464, Bucharest, RoumaniaUniversity of Agricultural Science and Veterinary Medicine of Cluj, 3-5 Calea Mănăştur Street, 400372, Cluj-Napoca, RoumaniaUniversity of Agricultural Science and Veterinary Medicine of Cluj, 3-5 Calea Mănăştur Street, 400372, Cluj-Napoca, RoumaniaUniversity of Agricultural Science and Veterinary Medicine of Cluj, 3-5 Calea Mănăştur Street, 400372, Cluj-Napoca, RoumaniaUniversity of Agricultural Science and Veterinary Medicine of Cluj, 3-5 Calea Mănăştur Street, 400372, Cluj-Napoca, RoumaniaAn experiment was conducted to evaluate the effects of Folligon and Corulon (hormons) supplemention during IVM on porcine oocyte maturation and subsequent fertilization with intracytoplasmic sperm injection , clevage and embryo development. Cumulus-oocyte complexes were cultured 44 hours with 10 IU Folligon and 10 IU Chorulon (0-44 h), or 22 hours withe the same amount of hormons (0-22 h) followed by a 22 hours cultue period without hormons). Nuclear maturation was assesed after IVM culture by examining the morphology and the presence of the first polar body using the micromanipulation system. The oocytes were injected with a single sperm cell and cultured in NCSU-23 medium. The percentage of cleaved embryos were determined, also the embryo development. The presence of Folligon and Chorulon during the first 22 hours of IVM gave beter results in the terms of polar body extrusion (63%) compared to the group exposed for 44 hours to the hormons (37%). After 6 days of culture , the number of advanced embyo development stage (more than 16 cells per embryo) was also significantly different in the 0-22 h group.https://spasb.ro/index.php/public_html/article/view/1276embryoicsimaturationoocyteporcine |
| spellingShingle | Iulian Roman Vasile Miclea Marius Zăhan Paul Tăpăloagă Andrea Hettig Ileana Miclea Alexandru Rusu Florin Ghiuru Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique Scientific Papers Animal Science and Biotechnologies embryo icsi maturation oocyte porcine |
| title | Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique |
| title_full | Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique |
| title_fullStr | Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique |
| title_full_unstemmed | Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique |
| title_short | Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique |
| title_sort | influence of in vitro maturation system of swine oocytes on embryo obtaining thru icsi tecnique |
| topic | embryo icsi maturation oocyte porcine |
| url | https://spasb.ro/index.php/public_html/article/view/1276 |
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