Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus
Diabetes mellitus (DM) affects the wound healing process, resulting in impaired healing or aberrant scarring. DM increases reactive oxygen species (ROS) production, fibroblast senescence and angiogenesis abnormalities, causing exacerbated inflammation accompanied by low levels of TGF—β and an increa...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/4/2201 |
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| author | Octavio Canales-Alvarez Maria Margarita Canales-Martinez Pilar Dominguez-Verano Daniela Balderas-Cordero Eduardo Madrigal-Bujaidar Isela Álvarez-González Marco Aurelio Rodriguez-Monroy |
| author_facet | Octavio Canales-Alvarez Maria Margarita Canales-Martinez Pilar Dominguez-Verano Daniela Balderas-Cordero Eduardo Madrigal-Bujaidar Isela Álvarez-González Marco Aurelio Rodriguez-Monroy |
| author_sort | Octavio Canales-Alvarez |
| collection | DOAJ |
| description | Diabetes mellitus (DM) affects the wound healing process, resulting in impaired healing or aberrant scarring. DM increases reactive oxygen species (ROS) production, fibroblast senescence and angiogenesis abnormalities, causing exacerbated inflammation accompanied by low levels of TGF—β and an increase in Matrix metalloproteinases (MMPs). Propolis has been proposed as a healing alternative for diabetic patients because it has antimicrobial, anti-inflammatory, antioxidant and proliferative effects and important properties in the healing process. An ethanolic extract of Chihuahua propolis (ChEEP) was obtained and fractionated, and the fractions were subjected to High–Performance Liquid Chromatography with diode–array (HPLC–DAD), High–Performance Liquid Chromatography–Mass Spectrometry (HPLC–MS) and Gas Chromatography–Mass Spectrometry (GC–MS) analyses and 46 compounds were detected. Deep wounds were made in a murine DM model induced by streptozotocin, and the speed of closure and the wound tensile strength were evaluated by the tensiometric method, which showed that ChEEP had similar activity to Recoveron, improving the speed of healing and increasing the wound tensile strength needed to open the wound again. A histological analysis of the wounds was performed using H&E staining, and when Matrix metalloproteinase 9 (MMP9) and α—actin were quantified by immunohistochemistry, ChEEP was shown to be associated with improved histological healing, as indicated by the reduced MMP9 and α—actin expression. In conclusion, topical ChEEP application enhances wound healing in diabetic mice. |
| first_indexed | 2024-03-07T22:28:44Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-07T22:28:44Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8636baa918d748bcbf9968ca1a2181912024-02-23T15:20:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254220110.3390/ijms25042201Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes MellitusOctavio Canales-Alvarez0Maria Margarita Canales-Martinez1Pilar Dominguez-Verano2Daniela Balderas-Cordero3Eduardo Madrigal-Bujaidar4Isela Álvarez-González5Marco Aurelio Rodriguez-Monroy6Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Zacatenco, Ciudad de México 07738, MexicoLaboratorio de Farmacognosia, UBIPRO, UNAM, FES Iztacala, Avenida de los Barrios Número 1, Tlalnepantla 54090, Estado de México, MexicoLaboratorio de Investigación Biomédica en Productos Naturales, Carrera de Medicina, UNAM, FES Iztacala, Avenida de los Barrios Número 1, Tlalnepantla 54090, Estado de México, MexicoLaboratorio de Investigación Biomédica en Productos Naturales, Carrera de Medicina, UNAM, FES Iztacala, Avenida de los Barrios Número 1, Tlalnepantla 54090, Estado de México, MexicoLaboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Zacatenco, Ciudad de México 07738, MexicoLaboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Zacatenco, Ciudad de México 07738, MexicoLaboratorio de Investigación Biomédica en Productos Naturales, Carrera de Medicina, UNAM, FES Iztacala, Avenida de los Barrios Número 1, Tlalnepantla 54090, Estado de México, MexicoDiabetes mellitus (DM) affects the wound healing process, resulting in impaired healing or aberrant scarring. DM increases reactive oxygen species (ROS) production, fibroblast senescence and angiogenesis abnormalities, causing exacerbated inflammation accompanied by low levels of TGF—β and an increase in Matrix metalloproteinases (MMPs). Propolis has been proposed as a healing alternative for diabetic patients because it has antimicrobial, anti-inflammatory, antioxidant and proliferative effects and important properties in the healing process. An ethanolic extract of Chihuahua propolis (ChEEP) was obtained and fractionated, and the fractions were subjected to High–Performance Liquid Chromatography with diode–array (HPLC–DAD), High–Performance Liquid Chromatography–Mass Spectrometry (HPLC–MS) and Gas Chromatography–Mass Spectrometry (GC–MS) analyses and 46 compounds were detected. Deep wounds were made in a murine DM model induced by streptozotocin, and the speed of closure and the wound tensile strength were evaluated by the tensiometric method, which showed that ChEEP had similar activity to Recoveron, improving the speed of healing and increasing the wound tensile strength needed to open the wound again. A histological analysis of the wounds was performed using H&E staining, and when Matrix metalloproteinase 9 (MMP9) and α—actin were quantified by immunohistochemistry, ChEEP was shown to be associated with improved histological healing, as indicated by the reduced MMP9 and α—actin expression. In conclusion, topical ChEEP application enhances wound healing in diabetic mice.https://www.mdpi.com/1422-0067/25/4/2201diabetes mellituswound healingpropolisinflammationantioxidant |
| spellingShingle | Octavio Canales-Alvarez Maria Margarita Canales-Martinez Pilar Dominguez-Verano Daniela Balderas-Cordero Eduardo Madrigal-Bujaidar Isela Álvarez-González Marco Aurelio Rodriguez-Monroy Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus International Journal of Molecular Sciences diabetes mellitus wound healing propolis inflammation antioxidant |
| title | Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus |
| title_full | Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus |
| title_fullStr | Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus |
| title_full_unstemmed | Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus |
| title_short | Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus |
| title_sort | effect of mexican propolis on wound healing in a murine model of diabetes mellitus |
| topic | diabetes mellitus wound healing propolis inflammation antioxidant |
| url | https://www.mdpi.com/1422-0067/25/4/2201 |
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