Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins
<p>Abstract</p> <p>Background</p> <p>The food-borne pathogen <it>Listeria monocytogenes</it> is the causative agent of listeriosis. The β-lactam antibiotics penicillin G and ampicillin are the current drugs of choice for the treatment of listerial infections...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/12/278 |
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| author | Krawczyk-Balska Agata Marchlewicz Julia Dudek Dorota Wasiak Katarzyna Samluk Anna |
| author_facet | Krawczyk-Balska Agata Marchlewicz Julia Dudek Dorota Wasiak Katarzyna Samluk Anna |
| author_sort | Krawczyk-Balska Agata |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The food-borne pathogen <it>Listeria monocytogenes</it> is the causative agent of listeriosis. The β-lactam antibiotics penicillin G and ampicillin are the current drugs of choice for the treatment of listerial infections. While isolates of <it>L</it>. <it>monocytogenes</it> are susceptible to these antibiotics, their action is only bacteriostatic and consequently, this bacterium is regarded as tolerant to β-lactams. In addition, <it>L</it>. <it>monocytogenes</it> has a high level of innate resistance to the cephalosporin family of β-lactams frequently used to treat sepsis of unknown etiology. Given the high mortality rate of listeriosis despite rational antibiotic therapy, it is important to identify genes that play a role in the susceptibility and tolerance of <it>L</it>. <it>monocytogenes</it> to β-lactams.</p> <p>Results</p> <p>The <it>hly</it>-based promoter trap system was applied to identify penicillin G-inducible genes of <it>L</it>. <it>monocytogenes</it>. The results of reporter system studies, verified by transcriptional analysis, identified ten penicillin G-inducible genes. The contribution of three of these genes, encoding a ferritin-like protein (<it>fri</it>), a two-component phosphate-response regulator (<it>phoP</it>) and an AraC/XylS family transcriptional regulator (<it>axyR</it>), to the susceptibility and tolerance of <it>L</it>. <it>monocytogenes</it> to β-lactams was examined by analysis of nonpolar deletion mutants. The absence of PhoP or AxyR resulted in more rapid growth of the strains in the presence of sublethal concentration of β-lactams, but had no effect on the MIC values or the ability to survive a lethal dose of these antibiotics. However, the Δ<it>fri</it> strain showed impaired growth in the presence of sublethal concentrations of penicillin G and ampicillin and a significantly reduced ability to survive lethal concentrations of these β-lactams. A lack of Fri also caused a 2-fold increase in the sensitivity of <it>L</it>. <it>monocytogenes</it> to cefalotin and cephradine.</p> <p>Conclusions</p> <p>The present study has identified Fri as an important mediator of β-lactam tolerance and innate resistance to cephalosporins in <it>L</it>. <it>monocytogenes</it>. PhoP and AxyR are probably involved in transmitting signals to adjust the rate of growth of <it>L</it>. <it>monocytogenes</it> under β-lactam pressure, but these regulators do not play a significant role in susceptibility and tolerance to this class of antibiotics.</p> |
| first_indexed | 2024-12-22T10:59:38Z |
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| language | English |
| last_indexed | 2024-12-22T10:59:38Z |
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| spelling | doaj.art-863d95ff37db43539812f427c069375a2022-12-21T18:28:33ZengBMCBMC Microbiology1471-21802012-11-0112127810.1186/1471-2180-12-278Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporinsKrawczyk-Balska AgataMarchlewicz JuliaDudek DorotaWasiak KatarzynaSamluk Anna<p>Abstract</p> <p>Background</p> <p>The food-borne pathogen <it>Listeria monocytogenes</it> is the causative agent of listeriosis. The β-lactam antibiotics penicillin G and ampicillin are the current drugs of choice for the treatment of listerial infections. While isolates of <it>L</it>. <it>monocytogenes</it> are susceptible to these antibiotics, their action is only bacteriostatic and consequently, this bacterium is regarded as tolerant to β-lactams. In addition, <it>L</it>. <it>monocytogenes</it> has a high level of innate resistance to the cephalosporin family of β-lactams frequently used to treat sepsis of unknown etiology. Given the high mortality rate of listeriosis despite rational antibiotic therapy, it is important to identify genes that play a role in the susceptibility and tolerance of <it>L</it>. <it>monocytogenes</it> to β-lactams.</p> <p>Results</p> <p>The <it>hly</it>-based promoter trap system was applied to identify penicillin G-inducible genes of <it>L</it>. <it>monocytogenes</it>. The results of reporter system studies, verified by transcriptional analysis, identified ten penicillin G-inducible genes. The contribution of three of these genes, encoding a ferritin-like protein (<it>fri</it>), a two-component phosphate-response regulator (<it>phoP</it>) and an AraC/XylS family transcriptional regulator (<it>axyR</it>), to the susceptibility and tolerance of <it>L</it>. <it>monocytogenes</it> to β-lactams was examined by analysis of nonpolar deletion mutants. The absence of PhoP or AxyR resulted in more rapid growth of the strains in the presence of sublethal concentration of β-lactams, but had no effect on the MIC values or the ability to survive a lethal dose of these antibiotics. However, the Δ<it>fri</it> strain showed impaired growth in the presence of sublethal concentrations of penicillin G and ampicillin and a significantly reduced ability to survive lethal concentrations of these β-lactams. A lack of Fri also caused a 2-fold increase in the sensitivity of <it>L</it>. <it>monocytogenes</it> to cefalotin and cephradine.</p> <p>Conclusions</p> <p>The present study has identified Fri as an important mediator of β-lactam tolerance and innate resistance to cephalosporins in <it>L</it>. <it>monocytogenes</it>. PhoP and AxyR are probably involved in transmitting signals to adjust the rate of growth of <it>L</it>. <it>monocytogenes</it> under β-lactam pressure, but these regulators do not play a significant role in susceptibility and tolerance to this class of antibiotics.</p>http://www.biomedcentral.com/1471-2180/12/278<it>Listeria monocytogenes</it>Tolerance to β-lactamsResistance to cephalosporins |
| spellingShingle | Krawczyk-Balska Agata Marchlewicz Julia Dudek Dorota Wasiak Katarzyna Samluk Anna Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins BMC Microbiology <it>Listeria monocytogenes</it> Tolerance to β-lactams Resistance to cephalosporins |
| title | Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins |
| title_full | Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins |
| title_fullStr | Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins |
| title_full_unstemmed | Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins |
| title_short | Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins |
| title_sort | identification of a ferritin like protein of it listeria monocytogenes it as a mediator of β lactam tolerance and innate resistance to cephalosporins |
| topic | <it>Listeria monocytogenes</it> Tolerance to β-lactams Resistance to cephalosporins |
| url | http://www.biomedcentral.com/1471-2180/12/278 |
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