An assay for beta-adrenergic receptors in isolated human fat cells.
The beta-adrenergic receptors have been characterized in isolated human adipocytes using a potent beta-adrenergic antagonist (-)-[3H]dihydroalprenolol. Binding of (-)-[3H]dihydroalprenolol to isolated fat cells was stereospecific and saturable, the maximum number of binding sites calculated being 7....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1982-07-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520381037 |
| _version_ | 1819055457780629504 |
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| author | P Engfeldt P Arner H Wahrenberg J Ostman |
| author_facet | P Engfeldt P Arner H Wahrenberg J Ostman |
| author_sort | P Engfeldt |
| collection | DOAJ |
| description | The beta-adrenergic receptors have been characterized in isolated human adipocytes using a potent beta-adrenergic antagonist (-)-[3H]dihydroalprenolol. Binding of (-)-[3H]dihydroalprenolol to isolated fat cells was stereospecific and saturable, the maximum number of binding sites calculated being 7.8 +/- 2.2 pmol of bound ligand/10(7) cells, corresponding to 450,000 binding sites/cell. The dissociation constant was estimated to be 2.7 +/- 1.1 nM. The results with competition-inhibition experiments using beta-adrenergic agonists and antagonists indicated that the binding sites in isolated adipocytes were predominantly of the beta1-subtype; about 80% of the receptors were of this type. With the present method, specific beta-adrenergic receptor number and affinity in isolated human adipocytes could be determined in about 1 g of human adipose tissue. |
| first_indexed | 2024-12-21T13:07:50Z |
| format | Article |
| id | doaj.art-8643633ab48945eea9fac9cb858f3fe7 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:07:50Z |
| publishDate | 1982-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-8643633ab48945eea9fac9cb858f3fe72022-12-21T19:02:57ZengElsevierJournal of Lipid Research0022-22751982-07-01235715719An assay for beta-adrenergic receptors in isolated human fat cells.P EngfeldtP ArnerH WahrenbergJ OstmanThe beta-adrenergic receptors have been characterized in isolated human adipocytes using a potent beta-adrenergic antagonist (-)-[3H]dihydroalprenolol. Binding of (-)-[3H]dihydroalprenolol to isolated fat cells was stereospecific and saturable, the maximum number of binding sites calculated being 7.8 +/- 2.2 pmol of bound ligand/10(7) cells, corresponding to 450,000 binding sites/cell. The dissociation constant was estimated to be 2.7 +/- 1.1 nM. The results with competition-inhibition experiments using beta-adrenergic agonists and antagonists indicated that the binding sites in isolated adipocytes were predominantly of the beta1-subtype; about 80% of the receptors were of this type. With the present method, specific beta-adrenergic receptor number and affinity in isolated human adipocytes could be determined in about 1 g of human adipose tissue.http://www.sciencedirect.com/science/article/pii/S0022227520381037 |
| spellingShingle | P Engfeldt P Arner H Wahrenberg J Ostman An assay for beta-adrenergic receptors in isolated human fat cells. Journal of Lipid Research |
| title | An assay for beta-adrenergic receptors in isolated human fat cells. |
| title_full | An assay for beta-adrenergic receptors in isolated human fat cells. |
| title_fullStr | An assay for beta-adrenergic receptors in isolated human fat cells. |
| title_full_unstemmed | An assay for beta-adrenergic receptors in isolated human fat cells. |
| title_short | An assay for beta-adrenergic receptors in isolated human fat cells. |
| title_sort | assay for beta adrenergic receptors in isolated human fat cells |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520381037 |
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