Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew
Polyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment results revealed similarity to po...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences
2019-10-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2019.129745 |
| Summary: | Polyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum
glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat
at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment
results revealed similarity to polyphenol oxidase (PPO) sequences from wheat, trifolium,
lettuce, apricot, tobacco, tomato, pokeweed, apple, grape and poplar especially at the
Copper B region of PPO. The 395 bp pearl millet PPO sequence was AT rich (53.3%) and
contained the highly conserved amino acids of histidine-rich copper binding sites similar
to PPO sequences from other crops. Results also indicated that PPO in pearl millet exists
in multi copy. The role of the isolated PPO gene during pearl millet-downy mildew interaction
was analyzed and the results showed significantly higher and rapid accumulation of
PPO mRNAs in resistant pearl millet seedlings inoculated with Sclerospora graminicola in
comparison to the susceptible control, demonstrating that the PPO plays a prominent role
in pearl millet defense against pathogens, particularly downy mildew pathogen. |
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| ISSN: | 1899-007X 1899-007X |