Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew
Polyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment results revealed similarity to po...
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2019-10-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2019.129745 |
| _version_ | 1818960825363202048 |
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| author | Sathyanarayana Niranjan-Raj Senapathyhally Nagaraju Lavanya Siddaiah Chandra Nayaka |
| author_facet | Sathyanarayana Niranjan-Raj Senapathyhally Nagaraju Lavanya Siddaiah Chandra Nayaka |
| author_sort | Sathyanarayana Niranjan-Raj |
| collection | DOAJ |
| description | Polyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum
glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat
at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment
results revealed similarity to polyphenol oxidase (PPO) sequences from wheat, trifolium,
lettuce, apricot, tobacco, tomato, pokeweed, apple, grape and poplar especially at the
Copper B region of PPO. The 395 bp pearl millet PPO sequence was AT rich (53.3%) and
contained the highly conserved amino acids of histidine-rich copper binding sites similar
to PPO sequences from other crops. Results also indicated that PPO in pearl millet exists
in multi copy. The role of the isolated PPO gene during pearl millet-downy mildew interaction
was analyzed and the results showed significantly higher and rapid accumulation of
PPO mRNAs in resistant pearl millet seedlings inoculated with Sclerospora graminicola in
comparison to the susceptible control, demonstrating that the PPO plays a prominent role
in pearl millet defense against pathogens, particularly downy mildew pathogen. |
| first_indexed | 2024-12-20T12:03:41Z |
| format | Article |
| id | doaj.art-8643b2e121934e7db459301d21ce1b5c |
| institution | Directory Open Access Journal |
| issn | 1899-007X 1899-007X |
| language | English |
| last_indexed | 2024-12-20T12:03:41Z |
| publishDate | 2019-10-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-8643b2e121934e7db459301d21ce1b5c2022-12-21T19:41:28ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2019-10-0159342342710.24425/jppr.2019.129745Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildewSathyanarayana Niranjan-Raj0Senapathyhally Nagaraju Lavanya1Siddaiah Chandra Nayaka2Sathyanarayana Niranjan-RajDepartment of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, IndiaDepartment of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, IndiaPolyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment results revealed similarity to polyphenol oxidase (PPO) sequences from wheat, trifolium, lettuce, apricot, tobacco, tomato, pokeweed, apple, grape and poplar especially at the Copper B region of PPO. The 395 bp pearl millet PPO sequence was AT rich (53.3%) and contained the highly conserved amino acids of histidine-rich copper binding sites similar to PPO sequences from other crops. Results also indicated that PPO in pearl millet exists in multi copy. The role of the isolated PPO gene during pearl millet-downy mildew interaction was analyzed and the results showed significantly higher and rapid accumulation of PPO mRNAs in resistant pearl millet seedlings inoculated with Sclerospora graminicola in comparison to the susceptible control, demonstrating that the PPO plays a prominent role in pearl millet defense against pathogens, particularly downy mildew pathogen.https://doi.org/10.24425/jppr.2019.129745downy mildewplant defensepennisetum glaucumpolyphenol oxidase |
| spellingShingle | Sathyanarayana Niranjan-Raj Senapathyhally Nagaraju Lavanya Siddaiah Chandra Nayaka Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew Journal of Plant Protection Research downy mildew plant defense pennisetum glaucum polyphenol oxidase |
| title | Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew |
| title_full | Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew |
| title_fullStr | Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew |
| title_full_unstemmed | Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew |
| title_short | Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew |
| title_sort | molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew |
| topic | downy mildew plant defense pennisetum glaucum polyphenol oxidase |
| url | https://doi.org/10.24425/jppr.2019.129745 |
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