Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on Metabolomics
Environmental factors affect plant metabolites, different climates, cultivation conditions, and biotic stresses and genotypes strongly affect their chemical composition and contents. Our aim is to examine the environmental and genetic interaction effects on tobacco metabolite composition. UPLC-QTOF...
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MDPI AG
2022-04-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/4/590 |
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| author | Bo Fu Junyang Liang Mengmeng Zhang Mingqin Zhao Xiaoming Ji Jing Wang |
| author_facet | Bo Fu Junyang Liang Mengmeng Zhang Mingqin Zhao Xiaoming Ji Jing Wang |
| author_sort | Bo Fu |
| collection | DOAJ |
| description | Environmental factors affect plant metabolites, different climates, cultivation conditions, and biotic stresses and genotypes strongly affect their chemical composition and contents. Our aim is to examine the environmental and genetic interaction effects on tobacco metabolite composition. UPLC-QTOF MS/MS coupled with multivariate data analyses were applied for the metabolomics analysis of three tobacco cultivars from different planting regions in China. Principal component analysis (PCA) revealed that environmental factors have a greater effect on tobacco metabolism compared to genotypes. Twelve biomarkers were screened by orthogonal partial least squares discrimination analysis (OPLS-DA). Univariate analysis indicated that Malate, conjugated chlorogenic acid, chlorogenic acid, quercetin 3-rutinoside-7-glucoside, and unknown compound <b>5</b> were only influenced by environmental factors (independent of genotype). Quinate, neochlorogenic acid, and ouabagenin, taxezopidine K1, taxezopidine K2, and taxezopidine K3 in tobacco were influenced by the interaction of environmental factors and the genotype. Our results suggest that metabolomics based on UPLC-QTOF MS/MS could be used to analyze the ecological functions of biomarker metabolites and understand the mechanisms of plant adaption to the environment. |
| first_indexed | 2024-03-09T10:32:09Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T10:32:09Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-86452f27d6cb4b9999782ef00f22ee372023-12-01T21:10:18ZengMDPI AGLife2075-17292022-04-0112459010.3390/life12040590Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on MetabolomicsBo Fu0Junyang Liang1Mengmeng Zhang2Mingqin Zhao3Xiaoming Ji4Jing Wang5College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, ChinaCollege of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, ChinaCollege of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, ChinaCollege of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, ChinaCollege of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, ChinaCollege of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, ChinaEnvironmental factors affect plant metabolites, different climates, cultivation conditions, and biotic stresses and genotypes strongly affect their chemical composition and contents. Our aim is to examine the environmental and genetic interaction effects on tobacco metabolite composition. UPLC-QTOF MS/MS coupled with multivariate data analyses were applied for the metabolomics analysis of three tobacco cultivars from different planting regions in China. Principal component analysis (PCA) revealed that environmental factors have a greater effect on tobacco metabolism compared to genotypes. Twelve biomarkers were screened by orthogonal partial least squares discrimination analysis (OPLS-DA). Univariate analysis indicated that Malate, conjugated chlorogenic acid, chlorogenic acid, quercetin 3-rutinoside-7-glucoside, and unknown compound <b>5</b> were only influenced by environmental factors (independent of genotype). Quinate, neochlorogenic acid, and ouabagenin, taxezopidine K1, taxezopidine K2, and taxezopidine K3 in tobacco were influenced by the interaction of environmental factors and the genotype. Our results suggest that metabolomics based on UPLC-QTOF MS/MS could be used to analyze the ecological functions of biomarker metabolites and understand the mechanisms of plant adaption to the environment.https://www.mdpi.com/2075-1729/12/4/590ecological functionsmetabolitesplanting regionsbiomarker metabolitesenvironment |
| spellingShingle | Bo Fu Junyang Liang Mengmeng Zhang Mingqin Zhao Xiaoming Ji Jing Wang Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on Metabolomics Life ecological functions metabolites planting regions biomarker metabolites environment |
| title | Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on Metabolomics |
| title_full | Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on Metabolomics |
| title_fullStr | Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on Metabolomics |
| title_full_unstemmed | Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on Metabolomics |
| title_short | Influence of Genotypic and Environmental Factors on Tobacco Leaves Based on Metabolomics |
| title_sort | influence of genotypic and environmental factors on tobacco leaves based on metabolomics |
| topic | ecological functions metabolites planting regions biomarker metabolites environment |
| url | https://www.mdpi.com/2075-1729/12/4/590 |
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