Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wal...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1020506/full |
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| author | A. Paterlini J. Sechet F. Immel M. S. Grison S. Pilard J. Pelloux G. Mouille E. M. Bayer A. Voxeur |
| author_facet | A. Paterlini J. Sechet F. Immel M. S. Grison S. Pilard J. Pelloux G. Mouille E. M. Bayer A. Voxeur |
| author_sort | A. Paterlini |
| collection | DOAJ |
| description | Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thaliana cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and enzymatic fingerprinting approaches. Relative to the rest of the cell wall, specific patterns were observed in the PD fraction. Most xyloglucans, although possibly not abundant as a group, were fucosylated. Homogalacturonans displayed short methylated stretches while rhamnogalacturonan I species were remarkably abundant. Full rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD. |
| first_indexed | 2024-04-11T07:25:32Z |
| format | Article |
| id | doaj.art-8646b8e3383542d9807764641bda4ca4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T07:25:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8646b8e3383542d9807764641bda4ca42022-12-22T04:37:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10205061020506Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmataA. Paterlini0J. Sechet1F. Immel2M. S. Grison3S. Pilard4J. Pelloux5G. Mouille6E. M. Bayer7A. Voxeur8Laboratoire de Biogenèse Membranaire, Unité mixte de recherche (UMR5200), Université Bordeaux, Centre national de la recherche scientifique (CNRS), Villenave d’Ornon, FranceInstitut Jean-Pierre Bourgin (IJPB), Université Paris-Saclay, Institut National de Recherche pour l'Agriculture, l'alimentation et l'Environnement (INRAE), AgroParisTech, Versailles, FranceLaboratoire de Biogenèse Membranaire, Unité mixte de recherche (UMR5200), Université Bordeaux, Centre national de la recherche scientifique (CNRS), Villenave d’Ornon, FranceLaboratoire de Biogenèse Membranaire, Unité mixte de recherche (UMR5200), Université Bordeaux, Centre national de la recherche scientifique (CNRS), Villenave d’Ornon, FrancePlateforme Analytique, Université de Picardie, Amiens, FranceUMRT (Unité Mixte de Recherche Transfrontaliére) INRAE (Institut National de recherche pour l'Agriculture, l'alimentation et l'Environnement) 1158 BioEcoAgro – BIOPI Biologie des Plantes et Innovation, Université de Picardie, Amiens, FranceInstitut Jean-Pierre Bourgin (IJPB), Université Paris-Saclay, Institut National de Recherche pour l'Agriculture, l'alimentation et l'Environnement (INRAE), AgroParisTech, Versailles, FranceLaboratoire de Biogenèse Membranaire, Unité mixte de recherche (UMR5200), Université Bordeaux, Centre national de la recherche scientifique (CNRS), Villenave d’Ornon, FranceInstitut Jean-Pierre Bourgin (IJPB), Université Paris-Saclay, Institut National de Recherche pour l'Agriculture, l'alimentation et l'Environnement (INRAE), AgroParisTech, Versailles, FrancePlasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thaliana cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and enzymatic fingerprinting approaches. Relative to the rest of the cell wall, specific patterns were observed in the PD fraction. Most xyloglucans, although possibly not abundant as a group, were fucosylated. Homogalacturonans displayed short methylated stretches while rhamnogalacturonan I species were remarkably abundant. Full rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD.https://www.frontiersin.org/articles/10.3389/fpls.2022.1020506/fullplasmodesmatacell wallArabidopsis thalianaenzymatic fingerprintingxyloglucanshomogalacturonans |
| spellingShingle | A. Paterlini J. Sechet F. Immel M. S. Grison S. Pilard J. Pelloux G. Mouille E. M. Bayer A. Voxeur Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata Frontiers in Plant Science plasmodesmata cell wall Arabidopsis thaliana enzymatic fingerprinting xyloglucans homogalacturonans |
| title | Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata |
| title_full | Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata |
| title_fullStr | Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata |
| title_full_unstemmed | Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata |
| title_short | Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata |
| title_sort | enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata |
| topic | plasmodesmata cell wall Arabidopsis thaliana enzymatic fingerprinting xyloglucans homogalacturonans |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1020506/full |
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