Immunohistochemical Analysis and Role of Secreted Frizzled-Related Protein-4 in Polycystic Ovary-Induced Rat
Objective: The role of Wnt signaling and its antagonist; secreted Frizzled Related Proteintype 4 (sFPR4) was reported in rodent ovarian follicular development. This study examinesimmunolocalization of sFRP4 in ovaries of polycystic ovary (PCO) rat model and evaluatesits role in follicular growth arr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2009-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/Article%202.pdf |
| Summary: | Objective: The role of Wnt signaling and its antagonist; secreted Frizzled Related Proteintype 4 (sFPR4) was reported in rodent ovarian follicular development. This study examinesimmunolocalization of sFRP4 in ovaries of polycystic ovary (PCO) rat model and evaluatesits role in follicular growth arrest and its premature differentiation.Materials and Mathods: PCO was induced with daily administration of testosterone propionate(TP) for 1 to 4 weeks while normal control rats were injected only with vehicle. Theovaries underwent histological examination, immunohistochemical analysis of sFRP4 andsteroidogenic acute regulatory protein (StAR) and apoptosis analysis.Results: Four-week TP treatment significantly increased the primordial follicles, and significantlydecreased the preantral and antral follicles compared to one week TP treatment.TP-treated animals had concomittantly, significant increase of sFPR4 immunoexpressionin primordial, primary and preantral follicles as compared to one week TP-treated animalsand control groups. Furthermore, sFRP4 immunostaining strongly co-localized in apoptoticgranulosa cells. Interestingly, increased sFRP4 immunostaining was associated withincreased StAR immunoexpression in follicular theca layer and stroma in four weeks TPtreatedrats compared to one week TP-treated rats and control groups.Conclusion: Our data showed a highly significant association between sFRP4 expressionand apoptosis in ovaries of four week TP-treated animals. Moreover, co-localizationof StAR and sFRP4 could suggest that sFRP4 may play a role in premature differentiationof follicles. |
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| ISSN: | 2228-5806 2228-5814 |