Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity
ABSTRACT: Herbal polysaccharides and their modifiers used as vaccine adjuvants have been widely investigated due to their safety and good immunoenhancing activity. In this study, the 50% ethanol concentration precipitated Lagenaria siceraria(Molina) standl polysaccharide (LSP50) and sulfated modifie...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122004011 |
| _version_ | 1811256277154660352 |
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| author | Adelijiang Wusiman Sabire Rexiati Mamat Aziz Xike Cheng Zhanhai Mai Alimire Abulaiti Abudureheimu Wutikuer Parhat Rozi Aytursun Abuduwaili Saifuding Abula |
| author_facet | Adelijiang Wusiman Sabire Rexiati Mamat Aziz Xike Cheng Zhanhai Mai Alimire Abulaiti Abudureheimu Wutikuer Parhat Rozi Aytursun Abuduwaili Saifuding Abula |
| author_sort | Adelijiang Wusiman |
| collection | DOAJ |
| description | ABSTRACT: Herbal polysaccharides and their modifiers used as vaccine adjuvants have been widely investigated due to their safety and good immunoenhancing activity. In this study, the 50% ethanol concentration precipitated Lagenaria siceraria(Molina) standl polysaccharide (LSP50) and sulfated modified LSP50 (sLSP50) was prepared, and their characterization was investigated. LSP50 and sLSP50-1.5 were used as vaccine adjuvants to immunize chickens, and the strength and type of immune responses induced by different adjuvants were detected. Our results showed that LSP50 was homogeneous polysaccharides, and the carbohydrate content was 98.6%. The sLSP50-1.5 with the DS value of 1.5 was optimized by response surface methodology. The sLSP50-1.5 has both characteristics of polysaccharide functional groups and sulfate functional groups. Adjuvant activity of LSP50 and sLSP50-1.5 showed that LSP50 and sLSP50-1.5 could induce long-lasting and high hemagglutination (HI) titers, antigen-specific lgG-NDV antibody, splenic lymphocyte proliferation, high immune organ index. Moreover, chicken immunized with sLSP50-1.5 showed a strong mixed Th1-type (IFN-γ and TNF-α) and Th2-type (IL-4 and IL-6) cytokines expression. Thus, these findings demonstrated that sLSP50-1.5 as a vaccine adjuvant can induce a mixed cellular and humoral immune response and can potentially serve as an effective vaccine adjuvant for NDV antigen. |
| first_indexed | 2024-04-12T17:37:39Z |
| format | Article |
| id | doaj.art-864bcce048fa4c83ad8e71bce2804cb1 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-12T17:37:39Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-864bcce048fa4c83ad8e71bce2804cb12022-12-22T03:22:54ZengElsevierPoultry Science0032-57912022-11-0110111102112Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activityAdelijiang Wusiman0Sabire Rexiati1Mamat Aziz2Xike Cheng3Zhanhai Mai4Alimire Abulaiti5Abudureheimu Wutikuer6Parhat Rozi7Aytursun Abuduwaili8Saifuding Abula9Xinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaXinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaXinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaXinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaXinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaXinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaXinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaChemical Engineering College, Xinjiang Agricultural University, Urumqi, 830000, PR ChinaXinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR China; Corresponding author:ABSTRACT: Herbal polysaccharides and their modifiers used as vaccine adjuvants have been widely investigated due to their safety and good immunoenhancing activity. In this study, the 50% ethanol concentration precipitated Lagenaria siceraria(Molina) standl polysaccharide (LSP50) and sulfated modified LSP50 (sLSP50) was prepared, and their characterization was investigated. LSP50 and sLSP50-1.5 were used as vaccine adjuvants to immunize chickens, and the strength and type of immune responses induced by different adjuvants were detected. Our results showed that LSP50 was homogeneous polysaccharides, and the carbohydrate content was 98.6%. The sLSP50-1.5 with the DS value of 1.5 was optimized by response surface methodology. The sLSP50-1.5 has both characteristics of polysaccharide functional groups and sulfate functional groups. Adjuvant activity of LSP50 and sLSP50-1.5 showed that LSP50 and sLSP50-1.5 could induce long-lasting and high hemagglutination (HI) titers, antigen-specific lgG-NDV antibody, splenic lymphocyte proliferation, high immune organ index. Moreover, chicken immunized with sLSP50-1.5 showed a strong mixed Th1-type (IFN-γ and TNF-α) and Th2-type (IL-4 and IL-6) cytokines expression. Thus, these findings demonstrated that sLSP50-1.5 as a vaccine adjuvant can induce a mixed cellular and humoral immune response and can potentially serve as an effective vaccine adjuvant for NDV antigen.http://www.sciencedirect.com/science/article/pii/S0032579122004011Lagenaria siceraria (Molina) Standl polysaccharidesulfated modifiedresponse surface methodvaccine adjuvants |
| spellingShingle | Adelijiang Wusiman Sabire Rexiati Mamat Aziz Xike Cheng Zhanhai Mai Alimire Abulaiti Abudureheimu Wutikuer Parhat Rozi Aytursun Abuduwaili Saifuding Abula Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity Poultry Science Lagenaria siceraria (Molina) Standl polysaccharide sulfated modified response surface method vaccine adjuvants |
| title | Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity |
| title_full | Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity |
| title_fullStr | Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity |
| title_full_unstemmed | Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity |
| title_short | Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity |
| title_sort | preparation and sulfate modified of lagenaria siceraria molina standl polysaccharide and its immune enhancing adjuvant activity |
| topic | Lagenaria siceraria (Molina) Standl polysaccharide sulfated modified response surface method vaccine adjuvants |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122004011 |
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