Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in Melon
Sexual differentiation is an important developmental phenomenon in cucurbits that directly affects fruit yield. The natural existence of multiple flower types in melon offers an inclusive structure for studying the molecular basis of sexual differentiation. The current study aimed to identify and ch...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/16905 |
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| author | Zhongyuan Wang Vivek Yadav Xiaoyao Chen Siyu Zhang Xinhao Yuan Hao Li Jianxiang Ma Yong Zhang Jianqiang Yang Xian Zhang Chunhua Wei |
| author_facet | Zhongyuan Wang Vivek Yadav Xiaoyao Chen Siyu Zhang Xinhao Yuan Hao Li Jianxiang Ma Yong Zhang Jianqiang Yang Xian Zhang Chunhua Wei |
| author_sort | Zhongyuan Wang |
| collection | DOAJ |
| description | Sexual differentiation is an important developmental phenomenon in cucurbits that directly affects fruit yield. The natural existence of multiple flower types in melon offers an inclusive structure for studying the molecular basis of sexual differentiation. The current study aimed to identify and characterize the molecular network involved in sex determination and female development in melon. Male and female pools separated by the F<sub>2</sub> segregated generation were used for sequencing. The comparative multi-omics data revealed 551 DAPs and 594 DEGs involved in multiple pathways of melon growth and development, and based on functional annotation and enrichment analysis, we summarized four biological process modules, including ethylene biosynthesis, flower organ development, plant hormone signaling, and ubiquitinated protein metabolism, that are related to female development. Furthermore, the detailed analysis of the female developmental regulatory pathway model of ethylene biosynthesis, signal transduction, and target gene regulation identified some important candidates that might have a crucial role in female development. Two CMTs ((cytosine-5)-methyltransferase), one AdoHS (adenosylhomocysteinase), four ACSs (1-aminocyclopropane-1-carboxylic acid synthase), three ACOs (ACC oxidase), two ARFs (auxin response factor), four ARPs (auxin-responsive protein), and six ERFs (Ethylene responsive factor) were identified based on various female developmental regulatory models. Our data offer new and valuable insights into female development and hold the potential to offer a deeper comprehension of sex differentiation mechanisms in melon. |
| first_indexed | 2024-03-09T01:50:00Z |
| format | Article |
| id | doaj.art-864cce194c2645ab814c5413a3eddb8e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:50:00Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-864cce194c2645ab814c5413a3eddb8e2023-12-08T15:17:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231690510.3390/ijms242316905Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in MelonZhongyuan Wang0Vivek Yadav1Xiaoyao Chen2Siyu Zhang3Xinhao Yuan4Hao Li5Jianxiang Ma6Yong Zhang7Jianqiang Yang8Xian Zhang9Chunhua Wei10State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaSexual differentiation is an important developmental phenomenon in cucurbits that directly affects fruit yield. The natural existence of multiple flower types in melon offers an inclusive structure for studying the molecular basis of sexual differentiation. The current study aimed to identify and characterize the molecular network involved in sex determination and female development in melon. Male and female pools separated by the F<sub>2</sub> segregated generation were used for sequencing. The comparative multi-omics data revealed 551 DAPs and 594 DEGs involved in multiple pathways of melon growth and development, and based on functional annotation and enrichment analysis, we summarized four biological process modules, including ethylene biosynthesis, flower organ development, plant hormone signaling, and ubiquitinated protein metabolism, that are related to female development. Furthermore, the detailed analysis of the female developmental regulatory pathway model of ethylene biosynthesis, signal transduction, and target gene regulation identified some important candidates that might have a crucial role in female development. Two CMTs ((cytosine-5)-methyltransferase), one AdoHS (adenosylhomocysteinase), four ACSs (1-aminocyclopropane-1-carboxylic acid synthase), three ACOs (ACC oxidase), two ARFs (auxin response factor), four ARPs (auxin-responsive protein), and six ERFs (Ethylene responsive factor) were identified based on various female developmental regulatory models. Our data offer new and valuable insights into female development and hold the potential to offer a deeper comprehension of sex differentiation mechanisms in melon.https://www.mdpi.com/1422-0067/24/23/16905cucurbitsethyleneomicssex differentiationfemale development |
| spellingShingle | Zhongyuan Wang Vivek Yadav Xiaoyao Chen Siyu Zhang Xinhao Yuan Hao Li Jianxiang Ma Yong Zhang Jianqiang Yang Xian Zhang Chunhua Wei Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in Melon International Journal of Molecular Sciences cucurbits ethylene omics sex differentiation female development |
| title | Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in Melon |
| title_full | Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in Melon |
| title_fullStr | Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in Melon |
| title_full_unstemmed | Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in Melon |
| title_short | Multi-Omics Analysis Reveals Intricate Gene Networks Involved in Female Development in Melon |
| title_sort | multi omics analysis reveals intricate gene networks involved in female development in melon |
| topic | cucurbits ethylene omics sex differentiation female development |
| url | https://www.mdpi.com/1422-0067/24/23/16905 |
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