IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors
Human lung mast cells (HLMCs) express the high-affinity receptor FcεRI for IgE and are strategically located in different compartments of human lung, where they play a role in several inflammatory disorders and cancer. Immunoglobulin superantigens (e.g., protein A of <i>Staphylococcus aureus&l...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/1/145 |
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| author | Leonardo Cristinziano Remo Poto Gjada Criscuolo Anne Lise Ferrara Maria Rosaria Galdiero Luca Modestino Stefania Loffredo Amato de Paulis Gianni Marone Giuseppe Spadaro Gilda Varricchi |
| author_facet | Leonardo Cristinziano Remo Poto Gjada Criscuolo Anne Lise Ferrara Maria Rosaria Galdiero Luca Modestino Stefania Loffredo Amato de Paulis Gianni Marone Giuseppe Spadaro Gilda Varricchi |
| author_sort | Leonardo Cristinziano |
| collection | DOAJ |
| description | Human lung mast cells (HLMCs) express the high-affinity receptor FcεRI for IgE and are strategically located in different compartments of human lung, where they play a role in several inflammatory disorders and cancer. Immunoglobulin superantigens (e.g., protein A of <i>Staphylococcus aureus</i> and protein L of <i>Peptostreptococcus magnus</i>) bind to the variable regions of either the heavy (V<sub>H</sub>3) or light chain (κ) of IgE. IL-33 is a cytokine expressed by epithelial cells that exerts pleiotropic functions in the lung. The present study investigated whether immunoglobulin superantigens protein A and protein L and IL-33 caused the release of inflammatory (histamine), angiogenic (VEGF-A) and lymphangiogenic (VEGF-C) factors from HLMCs. The results show that protein A and protein L induced the rapid (30 min) release of preformed histamine from HLMCs. By contrast, IL-33 did not induce the release of histamine from lung mast cells. Prolonged incubation (12 h) of HLMCs with superantigens and IL-33 induced the release of VEGF-A and VEGF-C. Preincubation with IL-33 potentiated the superantigenic release of histamine, angiogenic and lymphangiogenic factors from HLMCs. Our results suggest that IL-33 might enhance the inflammatory, angiogenic and lymphangiogenic activities of lung mast cells in pulmonary disorders. |
| first_indexed | 2024-03-09T05:02:58Z |
| format | Article |
| id | doaj.art-864d9880e6c744b8a7410f6dfc59fdaa |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T05:02:58Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-864d9880e6c744b8a7410f6dfc59fdaa2023-12-03T12:58:21ZengMDPI AGCells2073-44092021-01-0110114510.3390/cells10010145IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic FactorsLeonardo Cristinziano0Remo Poto1Gjada Criscuolo2Anne Lise Ferrara3Maria Rosaria Galdiero4Luca Modestino5Stefania Loffredo6Amato de Paulis7Gianni Marone8Giuseppe Spadaro9Gilda Varricchi10Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, ItalyHuman lung mast cells (HLMCs) express the high-affinity receptor FcεRI for IgE and are strategically located in different compartments of human lung, where they play a role in several inflammatory disorders and cancer. Immunoglobulin superantigens (e.g., protein A of <i>Staphylococcus aureus</i> and protein L of <i>Peptostreptococcus magnus</i>) bind to the variable regions of either the heavy (V<sub>H</sub>3) or light chain (κ) of IgE. IL-33 is a cytokine expressed by epithelial cells that exerts pleiotropic functions in the lung. The present study investigated whether immunoglobulin superantigens protein A and protein L and IL-33 caused the release of inflammatory (histamine), angiogenic (VEGF-A) and lymphangiogenic (VEGF-C) factors from HLMCs. The results show that protein A and protein L induced the rapid (30 min) release of preformed histamine from HLMCs. By contrast, IL-33 did not induce the release of histamine from lung mast cells. Prolonged incubation (12 h) of HLMCs with superantigens and IL-33 induced the release of VEGF-A and VEGF-C. Preincubation with IL-33 potentiated the superantigenic release of histamine, angiogenic and lymphangiogenic factors from HLMCs. Our results suggest that IL-33 might enhance the inflammatory, angiogenic and lymphangiogenic activities of lung mast cells in pulmonary disorders.https://www.mdpi.com/2073-4409/10/1/145allergyasthmahistamineIL-33mast cellprotein A |
| spellingShingle | Leonardo Cristinziano Remo Poto Gjada Criscuolo Anne Lise Ferrara Maria Rosaria Galdiero Luca Modestino Stefania Loffredo Amato de Paulis Gianni Marone Giuseppe Spadaro Gilda Varricchi IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors Cells allergy asthma histamine IL-33 mast cell protein A |
| title | IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors |
| title_full | IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors |
| title_fullStr | IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors |
| title_full_unstemmed | IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors |
| title_short | IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors |
| title_sort | il 33 and superantigenic activation of human lung mast cells induce the release of angiogenic and lymphangiogenic factors |
| topic | allergy asthma histamine IL-33 mast cell protein A |
| url | https://www.mdpi.com/2073-4409/10/1/145 |
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