Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
Background and Aim: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cy...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-07-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/July-2022/9.pdf |
| _version_ | 1818534148231397376 |
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| author | Ricaurte Lopera-Vásquez Fabián Uribe-García Iang Rondón-Barragán |
| author_facet | Ricaurte Lopera-Vásquez Fabián Uribe-García Iang Rondón-Barragán |
| author_sort | Ricaurte Lopera-Vásquez |
| collection | DOAJ |
| description | Background and Aim: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cycle phase. This study aimed to elucidate the effect of estrous cycle phases (follicular and early and late luteal phases) on gene expression patterns in bovine oviduct epithelial cells (BOECs).
Materials and Methods: Oviducts were obtained from healthy slaughterhouse animals, corresponding to ipsilateral ovaries with dominant follicles or corpus luteum during early and late luteal phases. BOECs were recovered from the isthmus (IST) and ampulla (AMP), and the expression patterns of genes related to cytokinesis and mitosis mechanisms (rho-associated coiled-coil containing protein kinase and cellular communication network factor 2 [CCN2]), growth factors (insulin-like growth factor-binding protein 3, epidermal growth factor receptor [EGFR], vascular endothelial growth factor A, and EGFR), antioxidant mechanisms (glutathione peroxidase 4 [GPX4]), apoptosis (B-cell lymphoma 2), complement component (C3), energy metabolism (aldose reductase gene family 1-member b1 [AKRIB1] and solute carrier family 2), hormone receptors (estrogen receptor 1 and luteinizing hormone/choriogonadotropin receptor), and specific glycoproteins (oviductal glycoprotein 1) were analyzed.
Results: High P4 levels (late luteal phase) affected the expression of important genes related to antioxidant mechanisms (GPX4), energy metabolism (AKRIB1), growth factors (IGBP3 and EGFR), and cell growth regulation (CCN2) in the AMP. Low P4 levels (early luteal phase) affected the expression of AKR1B1, IGBP3, and CCN2. In addition, estrogen likely had an effect on OVPGP expression in the cattle oviduct.
Conclusion: Differential gene expression patterns of BOECs in the AMP during the luteal phase (antioxidant mechanisms, energy metabolism, growth factors, and immunological regulators) and in the IST during the follicular phase (glycoproteins) may influence their renewal and population proportions, modulating the oviduct environment as well as gamete and embryo physiology. |
| first_indexed | 2024-12-11T18:07:57Z |
| format | Article |
| id | doaj.art-86509ca33ca64811b5c177114f33255b |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-11T18:07:57Z |
| publishDate | 2022-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-86509ca33ca64811b5c177114f33255b2022-12-22T00:55:39ZengVeterinary WorldVeterinary World0972-89882231-09162022-07-011571665167510.14202/vetworld.2022.1665-1675Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cellsRicaurte Lopera-Vásquez0https://orcid.org/0000-0001-6792-1961Fabián Uribe-García1https://orcid.org/0000-0001-9115-757XIang Rondón-Barragán2https://orcid.org/0000-0001-6980-892XImpronta Research Group, Faculty of Veterinary Medicine and Zootechnics. Universidad Cooperativa de Colombia, Ibagué-Tolima, Colombia.Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.Background and Aim: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cycle phase. This study aimed to elucidate the effect of estrous cycle phases (follicular and early and late luteal phases) on gene expression patterns in bovine oviduct epithelial cells (BOECs). Materials and Methods: Oviducts were obtained from healthy slaughterhouse animals, corresponding to ipsilateral ovaries with dominant follicles or corpus luteum during early and late luteal phases. BOECs were recovered from the isthmus (IST) and ampulla (AMP), and the expression patterns of genes related to cytokinesis and mitosis mechanisms (rho-associated coiled-coil containing protein kinase and cellular communication network factor 2 [CCN2]), growth factors (insulin-like growth factor-binding protein 3, epidermal growth factor receptor [EGFR], vascular endothelial growth factor A, and EGFR), antioxidant mechanisms (glutathione peroxidase 4 [GPX4]), apoptosis (B-cell lymphoma 2), complement component (C3), energy metabolism (aldose reductase gene family 1-member b1 [AKRIB1] and solute carrier family 2), hormone receptors (estrogen receptor 1 and luteinizing hormone/choriogonadotropin receptor), and specific glycoproteins (oviductal glycoprotein 1) were analyzed. Results: High P4 levels (late luteal phase) affected the expression of important genes related to antioxidant mechanisms (GPX4), energy metabolism (AKRIB1), growth factors (IGBP3 and EGFR), and cell growth regulation (CCN2) in the AMP. Low P4 levels (early luteal phase) affected the expression of AKR1B1, IGBP3, and CCN2. In addition, estrogen likely had an effect on OVPGP expression in the cattle oviduct. Conclusion: Differential gene expression patterns of BOECs in the AMP during the luteal phase (antioxidant mechanisms, energy metabolism, growth factors, and immunological regulators) and in the IST during the follicular phase (glycoproteins) may influence their renewal and population proportions, modulating the oviduct environment as well as gamete and embryo physiology.http://www.veterinaryworld.org/Vol.15/July-2022/9.pdfbovine oviduct epithelial cellsestrus phasefolliculargene expressionlutealoviduct |
| spellingShingle | Ricaurte Lopera-Vásquez Fabián Uribe-García Iang Rondón-Barragán Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells Veterinary World bovine oviduct epithelial cells estrus phase follicular gene expression luteal oviduct |
| title | Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells |
| title_full | Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells |
| title_fullStr | Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells |
| title_full_unstemmed | Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells |
| title_short | Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells |
| title_sort | effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells |
| topic | bovine oviduct epithelial cells estrus phase follicular gene expression luteal oviduct |
| url | http://www.veterinaryworld.org/Vol.15/July-2022/9.pdf |
| work_keys_str_mv | AT ricaurteloperavasquez effectofestrouscyclephasesongeneexpressioninbovineoviductepithelialcells AT fabianuribegarcia effectofestrouscyclephasesongeneexpressioninbovineoviductepithelialcells AT iangrondonbarragan effectofestrouscyclephasesongeneexpressioninbovineoviductepithelialcells |