Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging.
BACKGROUND: Cancer stem cells (CSCs) are highly tumorigenic and are responsible for tumor progression and chemoresistance. Noninvasive imaging methods for the visualization of CSC populations within tumors in vivo will have a considerable impact on the development of new CSC-targeting therapeutics....
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3534651?pdf=render |
| _version_ | 1811313674006036480 |
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| author | YoonSeok Choi Hoe Suk Kim Kyoung-Won Cho Kyung-Min Lee Yoon Jung Yi Sung-Jong Eun Hyun Jin Kim Jisu Woo Seung Hong Choi Taeg-Keun Whangbo ChulSoo Choi Dong-Young Noh Woo Kyung Moon |
| author_facet | YoonSeok Choi Hoe Suk Kim Kyoung-Won Cho Kyung-Min Lee Yoon Jung Yi Sung-Jong Eun Hyun Jin Kim Jisu Woo Seung Hong Choi Taeg-Keun Whangbo ChulSoo Choi Dong-Young Noh Woo Kyung Moon |
| author_sort | YoonSeok Choi |
| collection | DOAJ |
| description | BACKGROUND: Cancer stem cells (CSCs) are highly tumorigenic and are responsible for tumor progression and chemoresistance. Noninvasive imaging methods for the visualization of CSC populations within tumors in vivo will have a considerable impact on the development of new CSC-targeting therapeutics. METHODOLOGY/PRINCIPAL FINDINGS: In this study, human breast cancer stem cells (BCSCs) transduced with dual reporter genes (human ferritin heavy chain [FTH] and enhanced green fluorescence protein [EGFP]) were transplanted into NOD/SCID mice to allow noninvasive tracking of BCSC-derived populations. No changes in the properties of the BCSCs were observed due to ferritin overexpression. Magnetic resonance imaging (MRI) revealed significantly different signal intensities (R(2)* values) between BCSCs and FTH-BCSCs in vitro and in vivo. In addition, distinct populations of pixels with high R(2)* values were detected in docetaxel-treated FTH-BCSC tumors compared with control tumors, even before the tumor sizes changed. Histological analysis revealed that areas showing high R(2)* values in docetaxel-treated FTH-BCSC tumors by MRI contained EGFP+/FTH+ viable cell populations with high percentages of CD44+/CD24- cells. CONCLUSIONS/SIGNIFICANCE: These findings suggest that ferritin-based MRI, which provides high spatial resolution and tissue contrast, can be used as a reliable method to identify viable cell populations derived from BCSCs after chemotherapy and may serve as a new tool to monitor the efficacy of CSC-targeting therapies in vivo. |
| first_indexed | 2024-04-13T10:57:55Z |
| format | Article |
| id | doaj.art-865514b371b54024bd0bfdf23ce3cf4b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T10:57:55Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-865514b371b54024bd0bfdf23ce3cf4b2022-12-22T02:49:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5293110.1371/journal.pone.0052931Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging.YoonSeok ChoiHoe Suk KimKyoung-Won ChoKyung-Min LeeYoon Jung YiSung-Jong EunHyun Jin KimJisu WooSeung Hong ChoiTaeg-Keun WhangboChulSoo ChoiDong-Young NohWoo Kyung MoonBACKGROUND: Cancer stem cells (CSCs) are highly tumorigenic and are responsible for tumor progression and chemoresistance. Noninvasive imaging methods for the visualization of CSC populations within tumors in vivo will have a considerable impact on the development of new CSC-targeting therapeutics. METHODOLOGY/PRINCIPAL FINDINGS: In this study, human breast cancer stem cells (BCSCs) transduced with dual reporter genes (human ferritin heavy chain [FTH] and enhanced green fluorescence protein [EGFP]) were transplanted into NOD/SCID mice to allow noninvasive tracking of BCSC-derived populations. No changes in the properties of the BCSCs were observed due to ferritin overexpression. Magnetic resonance imaging (MRI) revealed significantly different signal intensities (R(2)* values) between BCSCs and FTH-BCSCs in vitro and in vivo. In addition, distinct populations of pixels with high R(2)* values were detected in docetaxel-treated FTH-BCSC tumors compared with control tumors, even before the tumor sizes changed. Histological analysis revealed that areas showing high R(2)* values in docetaxel-treated FTH-BCSC tumors by MRI contained EGFP+/FTH+ viable cell populations with high percentages of CD44+/CD24- cells. CONCLUSIONS/SIGNIFICANCE: These findings suggest that ferritin-based MRI, which provides high spatial resolution and tissue contrast, can be used as a reliable method to identify viable cell populations derived from BCSCs after chemotherapy and may serve as a new tool to monitor the efficacy of CSC-targeting therapies in vivo.http://europepmc.org/articles/PMC3534651?pdf=render |
| spellingShingle | YoonSeok Choi Hoe Suk Kim Kyoung-Won Cho Kyung-Min Lee Yoon Jung Yi Sung-Jong Eun Hyun Jin Kim Jisu Woo Seung Hong Choi Taeg-Keun Whangbo ChulSoo Choi Dong-Young Noh Woo Kyung Moon Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging. PLoS ONE |
| title | Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging. |
| title_full | Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging. |
| title_fullStr | Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging. |
| title_full_unstemmed | Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging. |
| title_short | Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging. |
| title_sort | noninvasive identification of viable cell populations in docetaxel treated breast tumors using ferritin based magnetic resonance imaging |
| url | http://europepmc.org/articles/PMC3534651?pdf=render |
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