Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins
Cryptosporidium parvum and Cryptosporidium hominis share highly similar proteomes, with merely ~3% divergence in overall nucleotide sequences. Cryptosporidium-specific MEDLE family is one of the major differences in gene content between the two species. Comparative genomic analysis indicated that ME...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01177/full |
| _version_ | 1819044441305907200 |
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| author | Jiayuan Su Chanchan Jin Haizhen Wu Jilan Fei Na Li Yaqiong Guo Yaoyu Feng Yaoyu Feng Lihua Xiao |
| author_facet | Jiayuan Su Chanchan Jin Haizhen Wu Jilan Fei Na Li Yaqiong Guo Yaoyu Feng Yaoyu Feng Lihua Xiao |
| author_sort | Jiayuan Su |
| collection | DOAJ |
| description | Cryptosporidium parvum and Cryptosporidium hominis share highly similar proteomes, with merely ~3% divergence in overall nucleotide sequences. Cryptosporidium-specific MEDLE family is one of the major differences in gene content between the two species. Comparative genomic analysis indicated that MEDLE family may contribute to differences in host range among Cryptosporidium spp. Previous studies have suggested that CpMEDLE-1 encoded by cgd5_4580 and CpMEDLE-2 encoded by cgd5_4590 are potentially involved in the invasion of C. parvum. In this study, we expressed in Escherichia coli, the C. hominis-specific member of the MEDLE protein family, ChMEDLE-1 encoded by chro.50507, and two C. parvum-specific members, CpMEDLE-3 encoded by cgd5_4600 and CpMEDLE-5 encoded by cgd6_5480. Quantitative PCR, immunofluorescence staining and in vitro neutralization assay were conducted to assess their biologic characteristics. The expression of the cgd5_4600 gene was high during 12–48 h of the in vitro culture, while the expression of cgd6_5480 was the highest at 2 h. ChMEDLE-1 and CpMEDLE-3 proteins were mostly located in the anterior and mid-anterior region of sporozoites and merozoites, whereas CpMEDLE-5 was expressed over the entire surface of these invasive stages. Polyclonal antibodies against MEDLE proteins had different neutralization efficiency, reaching approximately 50% for ChMEDLE-1 and 60% for CpMEDLE-3, but only 20% for CpMEDLE-5. The differences in protein and gene expression and neutralizing capacity indicated the MEDLE proteins may have different roles during Cryptosporidium invasion and growth. |
| first_indexed | 2024-12-21T10:12:44Z |
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| id | doaj.art-86563748c4684a22b09e6a893e04355b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T10:12:44Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-86563748c4684a22b09e6a893e04355b2022-12-21T19:07:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-05-011010.3389/fmicb.2019.01177460682Differential Expression of Three Cryptosporidium Species-Specific MEDLE ProteinsJiayuan Su0Chanchan Jin1Haizhen Wu2Jilan Fei3Na Li4Yaqiong Guo5Yaoyu Feng6Yaoyu Feng7Lihua Xiao8State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, ChinaState Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, ChinaSchool of Biotechnology, East China University of Science and Technology, Shanghai, ChinaState Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, ChinaKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaState Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, ChinaKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaCryptosporidium parvum and Cryptosporidium hominis share highly similar proteomes, with merely ~3% divergence in overall nucleotide sequences. Cryptosporidium-specific MEDLE family is one of the major differences in gene content between the two species. Comparative genomic analysis indicated that MEDLE family may contribute to differences in host range among Cryptosporidium spp. Previous studies have suggested that CpMEDLE-1 encoded by cgd5_4580 and CpMEDLE-2 encoded by cgd5_4590 are potentially involved in the invasion of C. parvum. In this study, we expressed in Escherichia coli, the C. hominis-specific member of the MEDLE protein family, ChMEDLE-1 encoded by chro.50507, and two C. parvum-specific members, CpMEDLE-3 encoded by cgd5_4600 and CpMEDLE-5 encoded by cgd6_5480. Quantitative PCR, immunofluorescence staining and in vitro neutralization assay were conducted to assess their biologic characteristics. The expression of the cgd5_4600 gene was high during 12–48 h of the in vitro culture, while the expression of cgd6_5480 was the highest at 2 h. ChMEDLE-1 and CpMEDLE-3 proteins were mostly located in the anterior and mid-anterior region of sporozoites and merozoites, whereas CpMEDLE-5 was expressed over the entire surface of these invasive stages. Polyclonal antibodies against MEDLE proteins had different neutralization efficiency, reaching approximately 50% for ChMEDLE-1 and 60% for CpMEDLE-3, but only 20% for CpMEDLE-5. The differences in protein and gene expression and neutralizing capacity indicated the MEDLE proteins may have different roles during Cryptosporidium invasion and growth.https://www.frontiersin.org/article/10.3389/fmicb.2019.01177/fullCryptosporidium parvumCryptosporidium hominisMEDLE familyinvasiongrowth |
| spellingShingle | Jiayuan Su Chanchan Jin Haizhen Wu Jilan Fei Na Li Yaqiong Guo Yaoyu Feng Yaoyu Feng Lihua Xiao Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins Frontiers in Microbiology Cryptosporidium parvum Cryptosporidium hominis MEDLE family invasion growth |
| title | Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins |
| title_full | Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins |
| title_fullStr | Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins |
| title_full_unstemmed | Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins |
| title_short | Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins |
| title_sort | differential expression of three cryptosporidium species specific medle proteins |
| topic | Cryptosporidium parvum Cryptosporidium hominis MEDLE family invasion growth |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01177/full |
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