Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings
Brown-marbled grouper Epinephelus fuscoguttatus (Forsskål) fingerlings are more vulnerable to diseases than the adult grouper because the fingerlings’ adaptive immune system is still in the development stage. The mortality rates are approximately 20 to 70% during outbreaks of vibriosis in aquacultur...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Inter-Research
2016-02-01
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| Series: | Aquaculture Environment Interactions |
| Online Access: | https://www.int-res.com/abstracts/aei/v8/p147-155/ |
| _version_ | 1818568753115299840 |
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| author | N Mohamed Alipiah NHS Ramli CF Low MN Shamsudin FM Yusoff |
| author_facet | N Mohamed Alipiah NHS Ramli CF Low MN Shamsudin FM Yusoff |
| author_sort | N Mohamed Alipiah |
| collection | DOAJ |
| description | Brown-marbled grouper Epinephelus fuscoguttatus (Forsskål) fingerlings are more vulnerable to diseases than the adult grouper because the fingerlings’ adaptive immune system is still in the development stage. The mortality rates are approximately 20 to 70% during outbreaks of vibriosis in aquacultures of grouper fingerlings. Studies were conducted to identify alternative treatments with low impacts on humans, animals and the environment as well as treatments that minimize the use of antibiotics in aquaculture. In this study, we report the first use of surface-associated bacteria isolated from marine invertebrates to increase fingerlings’ resistance against infections. Twenty-two surface-associated bacteria were isolated from the sea cucumber Stichopus badionotus and were identified with 16S rRNA gene sequencing. Three of the surface-associated bacteria had inhibitory activities against pathogenic Vibrio harveyi and V. parahaemolyticus. The fingerlings treated with the surface-associated bacteria Exiguobacterium acetylicum for 12 d prior to the challenge experiment with pathogenic V. harveyi showed significantly higher survival rates and increases in antibody titres compared to the control group. This study illustrats a symbiotic interaction between E. acetylicum and grouper fingerlings. E. acetylicum colonized the scales of grouper fingerlings and enhanced the fish’s immune response against the pathogen V. harveyi. |
| first_indexed | 2024-12-14T06:39:15Z |
| format | Article |
| id | doaj.art-8659903b71e44aefaa6c3fdfc05611d8 |
| institution | Directory Open Access Journal |
| issn | 1869-215X 1869-7534 |
| language | English |
| last_indexed | 2024-12-14T06:39:15Z |
| publishDate | 2016-02-01 |
| publisher | Inter-Research |
| record_format | Article |
| series | Aquaculture Environment Interactions |
| spelling | doaj.art-8659903b71e44aefaa6c3fdfc05611d82022-12-21T23:13:17ZengInter-ResearchAquaculture Environment Interactions1869-215X1869-75342016-02-01814715510.3354/aei00169Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlingsN Mohamed Alipiah0NHS Ramli1CF Low2MN Shamsudin3FM Yusoff4Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaLaboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaLaboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaLaboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaLaboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaBrown-marbled grouper Epinephelus fuscoguttatus (Forsskål) fingerlings are more vulnerable to diseases than the adult grouper because the fingerlings’ adaptive immune system is still in the development stage. The mortality rates are approximately 20 to 70% during outbreaks of vibriosis in aquacultures of grouper fingerlings. Studies were conducted to identify alternative treatments with low impacts on humans, animals and the environment as well as treatments that minimize the use of antibiotics in aquaculture. In this study, we report the first use of surface-associated bacteria isolated from marine invertebrates to increase fingerlings’ resistance against infections. Twenty-two surface-associated bacteria were isolated from the sea cucumber Stichopus badionotus and were identified with 16S rRNA gene sequencing. Three of the surface-associated bacteria had inhibitory activities against pathogenic Vibrio harveyi and V. parahaemolyticus. The fingerlings treated with the surface-associated bacteria Exiguobacterium acetylicum for 12 d prior to the challenge experiment with pathogenic V. harveyi showed significantly higher survival rates and increases in antibody titres compared to the control group. This study illustrats a symbiotic interaction between E. acetylicum and grouper fingerlings. E. acetylicum colonized the scales of grouper fingerlings and enhanced the fish’s immune response against the pathogen V. harveyi.https://www.int-res.com/abstracts/aei/v8/p147-155/ |
| spellingShingle | N Mohamed Alipiah NHS Ramli CF Low MN Shamsudin FM Yusoff Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings Aquaculture Environment Interactions |
| title | Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings |
| title_full | Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings |
| title_fullStr | Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings |
| title_full_unstemmed | Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings |
| title_short | Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings |
| title_sort | protective effects of sea cucumber surface associated bacteria against vibrio harveyi in brown marbled grouper fingerlings |
| url | https://www.int-res.com/abstracts/aei/v8/p147-155/ |
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