Influence of probiotic microorganisms on microbial biofilms in feeds
At different stages of feed production and storage, it is possible to contaminate both feed and their components with various pathogenic and opportunistic microorganisms that can cause infectious diseases not only among animals but also have epidemiological significance. The aim of the study was to...
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| Format: | Article |
| Language: | English |
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Zhytomyr National Agroecological University
2022-05-01
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| Series: | Наукові горизонти |
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| Online Access: | https://sciencehorizon.com.ua/en/journals/tom-25-1-2022/vpliv-probiotichnikh-mikroorganizmiv-na-mikrobni-bioplivki-u-kormakh |
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| author | Olena Kolchyk Тetiana Illarionova Andriy Buzun Anatoliy Paliy Andrii Palii |
| author_facet | Olena Kolchyk Тetiana Illarionova Andriy Buzun Anatoliy Paliy Andrii Palii |
| author_sort | Olena Kolchyk |
| collection | DOAJ |
| description | At different stages of feed production and storage, it is possible to contaminate both feed and their components with various pathogenic and opportunistic microorganisms that can cause infectious diseases not only among animals but also have epidemiological significance. The aim of the study was to isolate biofilm-forming strains of microorganisms from feed, as well as to study the inhibitory activity of the probiotic complex of bacteria of the genus Bacillus against microbial biofilms. Identification and species affiliation of isolated bacterial field isolates were performed by cultural-morphological and biochemical properties. The formation of biofilms was studied by determining the ability of isolates of microbial associations and individual species of microorganisms to adhere to the surface of a 96-well polystyrene tablet according to the method of O'Toole & Kolter, 1998. Determination of microbial contamination of 50 industrial batches of feed from 4 pig farms in two regions of Ukraine (barley, compound feed SK-31 for rearing, SK-51 for fattening pigs, EXCELL starter for pigs 15%, shop prestarter, compound feed for lactating sows). In 11 experimental batches of barley (68.8%) and 13 batches of 3 types of feed (SK-31, SK-51, feed for lactating sows) identified associations with different microorganisms Pasteurella multocida, Corynebacterium striatum, Bacillus subtilis, Leptothrix ochracea, Haemophilus parasuis and yeast Candida albicans. The association of Actinobacillus pleuropneumonia bacteria with B. subtilis was identified in 2 batches (50%) of the shop prestarter. Moderate, by optical density, biofilm formation for associations of microorganisms P. multocida + C. striatum + C. albicans (D620=3.59) and P. multocida + L. ochracea + C. albicans (D620=3.62). Planktonic forms of C. striatum and P. multocida showed low film-forming activity at the level (D620<1.51). Inhibitory activity of the probiotic complex of bacteria of the genus Bacillus (B. subtilis, B. licheniformis, B. amyloliquefaciens) was determined by isolated variations of microbial biofilms in 5 feed species, which displaced all biofilm-forming microorganisms except H. parasuis |
| first_indexed | 2024-03-13T01:14:42Z |
| format | Article |
| id | doaj.art-865b8f0b4e604de8b4c1ca848661ed8d |
| institution | Directory Open Access Journal |
| issn | 2663-2144 |
| language | English |
| last_indexed | 2024-03-13T01:14:42Z |
| publishDate | 2022-05-01 |
| publisher | Zhytomyr National Agroecological University |
| record_format | Article |
| series | Наукові горизонти |
| spelling | doaj.art-865b8f0b4e604de8b4c1ca848661ed8d2023-07-05T14:11:58ZengZhytomyr National Agroecological UniversityНаукові горизонти2663-21442022-05-01251415010.48077/scihor.25(1).2022.41-50Influence of probiotic microorganisms on microbial biofilms in feedsOlena Kolchyk0Тetiana Illarionova1Andriy Buzun2Anatoliy Paliy3Andrii Palii4National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine” 61023, 83 Pushkinska Str., Kharkiv, UkraineLimited Liability Company “Sіrіоn” 49027, 18 Akinfeeva Str., Dnipro, UkraineNational Scientific Center “Institute of Experimental and Clinical Veterinary Medicine” 61023, 83 Pushkinska Str., Kharkiv, UkraineScientific Center “Institute of Experimental and Clinical Veterinary Medicine” 61023, 83 Pushkinska Str., Kharkiv, UkraineState Biotechnological University 61002, 44 Alchevskyh Str., Kharkiv, UkraineAt different stages of feed production and storage, it is possible to contaminate both feed and their components with various pathogenic and opportunistic microorganisms that can cause infectious diseases not only among animals but also have epidemiological significance. The aim of the study was to isolate biofilm-forming strains of microorganisms from feed, as well as to study the inhibitory activity of the probiotic complex of bacteria of the genus Bacillus against microbial biofilms. Identification and species affiliation of isolated bacterial field isolates were performed by cultural-morphological and biochemical properties. The formation of biofilms was studied by determining the ability of isolates of microbial associations and individual species of microorganisms to adhere to the surface of a 96-well polystyrene tablet according to the method of O'Toole & Kolter, 1998. Determination of microbial contamination of 50 industrial batches of feed from 4 pig farms in two regions of Ukraine (barley, compound feed SK-31 for rearing, SK-51 for fattening pigs, EXCELL starter for pigs 15%, shop prestarter, compound feed for lactating sows). In 11 experimental batches of barley (68.8%) and 13 batches of 3 types of feed (SK-31, SK-51, feed for lactating sows) identified associations with different microorganisms Pasteurella multocida, Corynebacterium striatum, Bacillus subtilis, Leptothrix ochracea, Haemophilus parasuis and yeast Candida albicans. The association of Actinobacillus pleuropneumonia bacteria with B. subtilis was identified in 2 batches (50%) of the shop prestarter. Moderate, by optical density, biofilm formation for associations of microorganisms P. multocida + C. striatum + C. albicans (D620=3.59) and P. multocida + L. ochracea + C. albicans (D620=3.62). Planktonic forms of C. striatum and P. multocida showed low film-forming activity at the level (D620<1.51). Inhibitory activity of the probiotic complex of bacteria of the genus Bacillus (B. subtilis, B. licheniformis, B. amyloliquefaciens) was determined by isolated variations of microbial biofilms in 5 feed species, which displaced all biofilm-forming microorganisms except H. parasuishttps://sciencehorizon.com.ua/en/journals/tom-25-1-2022/vpliv-probiotichnikh-mikroorganizmiv-na-mikrobni-bioplivki-u-kormakhbiofilm-forming microorganismsplanktonic bacteriamicrobial contamination of foodprobiotic complex of bacteria of the genus bacillusinhibitory activit |
| spellingShingle | Olena Kolchyk Тetiana Illarionova Andriy Buzun Anatoliy Paliy Andrii Palii Influence of probiotic microorganisms on microbial biofilms in feeds Наукові горизонти biofilm-forming microorganisms planktonic bacteria microbial contamination of food probiotic complex of bacteria of the genus bacillus inhibitory activit |
| title | Influence of probiotic microorganisms on microbial biofilms in feeds |
| title_full | Influence of probiotic microorganisms on microbial biofilms in feeds |
| title_fullStr | Influence of probiotic microorganisms on microbial biofilms in feeds |
| title_full_unstemmed | Influence of probiotic microorganisms on microbial biofilms in feeds |
| title_short | Influence of probiotic microorganisms on microbial biofilms in feeds |
| title_sort | influence of probiotic microorganisms on microbial biofilms in feeds |
| topic | biofilm-forming microorganisms planktonic bacteria microbial contamination of food probiotic complex of bacteria of the genus bacillus inhibitory activit |
| url | https://sciencehorizon.com.ua/en/journals/tom-25-1-2022/vpliv-probiotichnikh-mikroorganizmiv-na-mikrobni-bioplivki-u-kormakh |
| work_keys_str_mv | AT olenakolchyk influenceofprobioticmicroorganismsonmicrobialbiofilmsinfeeds AT tetianaillarionova influenceofprobioticmicroorganismsonmicrobialbiofilmsinfeeds AT andriybuzun influenceofprobioticmicroorganismsonmicrobialbiofilmsinfeeds AT anatoliypaliy influenceofprobioticmicroorganismsonmicrobialbiofilmsinfeeds AT andriipalii influenceofprobioticmicroorganismsonmicrobialbiofilmsinfeeds |